Amino acid dipepetide frequency for Rhodobacter phage RcTitan

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.119AlaAla: 19.119 ± 3.921
1.191AlaCys: 1.191 ± 0.257
5.463AlaAsp: 5.463 ± 0.626
7.704AlaGlu: 7.704 ± 0.88
5.393AlaPhe: 5.393 ± 0.562
10.505AlaGly: 10.505 ± 1.179
1.191AlaHis: 1.191 ± 0.286
5.603AlaIle: 5.603 ± 0.576
6.373AlaLys: 6.373 ± 1.03
10.295AlaLeu: 10.295 ± 0.961
2.031AlaMet: 2.031 ± 0.413
5.112AlaAsn: 5.112 ± 0.735
4.972AlaPro: 4.972 ± 0.71
5.323AlaGln: 5.323 ± 1.134
5.533AlaArg: 5.533 ± 0.655
8.124AlaSer: 8.124 ± 0.949
9.244AlaThr: 9.244 ± 1.325
8.614AlaVal: 8.614 ± 1.006
1.121AlaTrp: 1.121 ± 0.277
2.591AlaTyr: 2.591 ± 0.385
0.0AlaXaa: 0.0 ± 0.0
Cys
1.05CysAla: 1.05 ± 0.287
0.07CysCys: 0.07 ± 0.06
0.77CysAsp: 0.77 ± 0.213
0.7CysGlu: 0.7 ± 0.227
0.49CysPhe: 0.49 ± 0.212
1.261CysGly: 1.261 ± 0.36
0.56CysHis: 0.56 ± 0.195
0.42CysIle: 0.42 ± 0.192
0.42CysLys: 0.42 ± 0.232
0.56CysLeu: 0.56 ± 0.164
0.07CysMet: 0.07 ± 0.076
0.63CysAsn: 0.63 ± 0.217
0.35CysPro: 0.35 ± 0.157
0.14CysGln: 0.14 ± 0.104
0.7CysArg: 0.7 ± 0.228
0.49CysSer: 0.49 ± 0.154
0.28CysThr: 0.28 ± 0.13
0.21CysVal: 0.21 ± 0.132
0.14CysTrp: 0.14 ± 0.102
0.28CysTyr: 0.28 ± 0.134
0.0CysXaa: 0.0 ± 0.0
Asp
6.233AspAla: 6.233 ± 0.681
0.84AspCys: 0.84 ± 0.271
3.922AspAsp: 3.922 ± 0.616
3.502AspGlu: 3.502 ± 0.482
2.801AspPhe: 2.801 ± 0.485
5.112AspGly: 5.112 ± 0.723
0.49AspHis: 0.49 ± 0.188
2.801AspIle: 2.801 ± 0.477
3.081AspLys: 3.081 ± 0.436
5.042AspLeu: 5.042 ± 0.551
1.331AspMet: 1.331 ± 0.334
2.241AspAsn: 2.241 ± 0.369
3.222AspPro: 3.222 ± 0.503
1.05AspGln: 1.05 ± 0.311
3.362AspArg: 3.362 ± 0.576
1.191AspSer: 1.191 ± 0.226
2.171AspThr: 2.171 ± 0.325
3.362AspVal: 3.362 ± 0.436
0.77AspTrp: 0.77 ± 0.241
1.681AspTyr: 1.681 ± 0.382
0.0AspXaa: 0.0 ± 0.0
Glu
9.104GluAla: 9.104 ± 1.031
0.56GluCys: 0.56 ± 0.182
3.151GluAsp: 3.151 ± 0.473
3.992GluGlu: 3.992 ± 0.685
1.751GluPhe: 1.751 ± 0.317
3.992GluGly: 3.992 ± 0.458
0.7GluHis: 0.7 ± 0.205
4.202GluIle: 4.202 ± 0.486
3.502GluLys: 3.502 ± 0.566
5.743GluLeu: 5.743 ± 0.757
2.451GluMet: 2.451 ± 0.42
2.381GluAsn: 2.381 ± 0.377
1.891GluPro: 1.891 ± 0.354
2.801GluGln: 2.801 ± 0.683
3.151GluArg: 3.151 ± 0.502
2.031GluSer: 2.031 ± 0.314
3.992GluThr: 3.992 ± 0.591
3.292GluVal: 3.292 ± 0.432
1.121GluTrp: 1.121 ± 0.297
2.661GluTyr: 2.661 ± 0.36
0.0GluXaa: 0.0 ± 0.0
Phe
5.883PheAla: 5.883 ± 0.567
0.42PheCys: 0.42 ± 0.145
3.011PheAsp: 3.011 ± 0.47
3.992PheGlu: 3.992 ± 0.496
1.961PhePhe: 1.961 ± 0.34
3.502PheGly: 3.502 ± 0.402
0.35PheHis: 0.35 ± 0.149
2.521PheIle: 2.521 ± 0.497
2.031PheLys: 2.031 ± 0.348
1.891PheLeu: 1.891 ± 0.32
0.91PheMet: 0.91 ± 0.233
1.541PheAsn: 1.541 ± 0.245
1.891PhePro: 1.891 ± 0.342
1.05PheGln: 1.05 ± 0.256
2.381PheArg: 2.381 ± 0.407
1.891PheSer: 1.891 ± 0.38
2.521PheThr: 2.521 ± 0.431
3.992PheVal: 3.992 ± 0.539
0.49PheTrp: 0.49 ± 0.184
1.121PheTyr: 1.121 ± 0.266
0.0PheXaa: 0.0 ± 0.0
Gly
9.735GlyAla: 9.735 ± 1.363
0.98GlyCys: 0.98 ± 0.322
4.622GlyAsp: 4.622 ± 0.633
4.622GlyGlu: 4.622 ± 0.529
4.412GlyPhe: 4.412 ± 0.422
7.704GlyGly: 7.704 ± 1.608
1.05GlyHis: 1.05 ± 0.315
3.151GlyIle: 3.151 ± 0.574
4.902GlyLys: 4.902 ± 0.578
5.393GlyLeu: 5.393 ± 0.702
1.191GlyMet: 1.191 ± 0.338
2.661GlyAsn: 2.661 ± 0.401
3.081GlyPro: 3.081 ± 0.458
3.362GlyGln: 3.362 ± 0.799
3.642GlyArg: 3.642 ± 0.546
5.603GlySer: 5.603 ± 0.97
4.762GlyThr: 4.762 ± 0.847
7.003GlyVal: 7.003 ± 0.8
1.05GlyTrp: 1.05 ± 0.393
2.661GlyTyr: 2.661 ± 0.546
0.0GlyXaa: 0.0 ± 0.0
His
0.91HisAla: 0.91 ± 0.252
0.14HisCys: 0.14 ± 0.108
0.56HisAsp: 0.56 ± 0.23
0.56HisGlu: 0.56 ± 0.179
0.42HisPhe: 0.42 ± 0.169
1.05HisGly: 1.05 ± 0.275
0.07HisHis: 0.07 ± 0.075
1.121HisIle: 1.121 ± 0.294
0.42HisLys: 0.42 ± 0.188
0.84HisLeu: 0.84 ± 0.216
0.28HisMet: 0.28 ± 0.119
0.84HisAsn: 0.84 ± 0.303
0.49HisPro: 0.49 ± 0.216
0.0HisGln: 0.0 ± 0.0
0.42HisArg: 0.42 ± 0.158
1.121HisSer: 1.121 ± 0.303
0.28HisThr: 0.28 ± 0.152
1.121HisVal: 1.121 ± 0.234
0.14HisTrp: 0.14 ± 0.135
0.42HisTyr: 0.42 ± 0.175
0.0HisXaa: 0.0 ± 0.0
Ile
7.143IleAla: 7.143 ± 0.57
0.7IleCys: 0.7 ± 0.213
3.151IleAsp: 3.151 ± 0.668
4.202IleGlu: 4.202 ± 0.489
1.331IlePhe: 1.331 ± 0.294
3.852IleGly: 3.852 ± 0.517
0.49IleHis: 0.49 ± 0.206
2.171IleIle: 2.171 ± 0.427
2.451IleLys: 2.451 ± 0.508
2.451IleLeu: 2.451 ± 0.379
1.05IleMet: 1.05 ± 0.193
2.241IleAsn: 2.241 ± 0.361
2.661IlePro: 2.661 ± 0.4
0.98IleGln: 0.98 ± 0.314
3.081IleArg: 3.081 ± 0.475
3.362IleSer: 3.362 ± 0.478
3.362IleThr: 3.362 ± 0.598
3.572IleVal: 3.572 ± 0.77
0.28IleTrp: 0.28 ± 0.125
0.98IleTyr: 0.98 ± 0.263
0.0IleXaa: 0.0 ± 0.0
Lys
7.073LysAla: 7.073 ± 0.899
0.56LysCys: 0.56 ± 0.233
2.451LysAsp: 2.451 ± 0.504
3.642LysGlu: 3.642 ± 0.614
1.891LysPhe: 1.891 ± 0.434
3.432LysGly: 3.432 ± 0.437
0.84LysHis: 0.84 ± 0.263
2.941LysIle: 2.941 ± 0.577
3.432LysLys: 3.432 ± 0.611
4.832LysLeu: 4.832 ± 0.539
1.261LysMet: 1.261 ± 0.31
2.171LysAsn: 2.171 ± 0.416
3.852LysPro: 3.852 ± 0.723
1.821LysGln: 1.821 ± 0.236
3.151LysArg: 3.151 ± 0.593
3.081LysSer: 3.081 ± 0.473
3.642LysThr: 3.642 ± 0.444
2.871LysVal: 2.871 ± 0.412
0.49LysTrp: 0.49 ± 0.238
1.261LysTyr: 1.261 ± 0.283
0.0LysXaa: 0.0 ± 0.0
Leu
7.984LeuAla: 7.984 ± 0.808
0.35LeuCys: 0.35 ± 0.181
4.692LeuAsp: 4.692 ± 0.703
3.081LeuGlu: 3.081 ± 0.436
3.222LeuPhe: 3.222 ± 0.455
4.832LeuGly: 4.832 ± 0.486
1.191LeuHis: 1.191 ± 0.348
3.642LeuIle: 3.642 ± 0.451
4.482LeuLys: 4.482 ± 0.492
4.832LeuLeu: 4.832 ± 0.549
1.821LeuMet: 1.821 ± 0.387
3.712LeuAsn: 3.712 ± 0.481
3.222LeuPro: 3.222 ± 0.51
3.362LeuGln: 3.362 ± 0.361
4.902LeuArg: 4.902 ± 0.682
5.533LeuSer: 5.533 ± 0.563
5.393LeuThr: 5.393 ± 0.61
4.132LeuVal: 4.132 ± 0.708
1.261LeuTrp: 1.261 ± 0.346
2.731LeuTyr: 2.731 ± 0.462
0.0LeuXaa: 0.0 ± 0.0
Met
2.731MetAla: 2.731 ± 0.536
0.14MetCys: 0.14 ± 0.111
0.35MetAsp: 0.35 ± 0.153
0.56MetGlu: 0.56 ± 0.194
1.611MetPhe: 1.611 ± 0.272
1.751MetGly: 1.751 ± 0.446
0.28MetHis: 0.28 ± 0.172
0.91MetIle: 0.91 ± 0.272
1.471MetLys: 1.471 ± 0.379
2.101MetLeu: 2.101 ± 0.435
0.14MetMet: 0.14 ± 0.112
1.471MetAsn: 1.471 ± 0.294
1.681MetPro: 1.681 ± 0.324
1.121MetGln: 1.121 ± 0.294
1.05MetArg: 1.05 ± 0.225
2.031MetSer: 2.031 ± 0.398
2.311MetThr: 2.311 ± 0.344
0.98MetVal: 0.98 ± 0.235
0.07MetTrp: 0.07 ± 0.073
0.28MetTyr: 0.28 ± 0.117
0.0MetXaa: 0.0 ± 0.0
Asn
5.883AsnAla: 5.883 ± 0.71
0.63AsnCys: 0.63 ± 0.23
2.521AsnAsp: 2.521 ± 0.479
3.572AsnGlu: 3.572 ± 0.43
1.471AsnPhe: 1.471 ± 0.381
4.272AsnGly: 4.272 ± 0.451
0.56AsnHis: 0.56 ± 0.211
1.541AsnIle: 1.541 ± 0.268
1.961AsnLys: 1.961 ± 0.365
2.591AsnLeu: 2.591 ± 0.356
1.05AsnMet: 1.05 ± 0.241
2.311AsnAsn: 2.311 ± 0.312
3.292AsnPro: 3.292 ± 0.407
1.05AsnGln: 1.05 ± 0.275
2.941AsnArg: 2.941 ± 0.492
1.541AsnSer: 1.541 ± 0.322
1.681AsnThr: 1.681 ± 0.245
3.642AsnVal: 3.642 ± 0.451
0.56AsnTrp: 0.56 ± 0.205
0.91AsnTyr: 0.91 ± 0.236
0.0AsnXaa: 0.0 ± 0.0
Pro
6.093ProAla: 6.093 ± 0.924
0.21ProCys: 0.21 ± 0.161
3.502ProAsp: 3.502 ± 0.654
3.292ProGlu: 3.292 ± 0.501
2.241ProPhe: 2.241 ± 0.446
3.852ProGly: 3.852 ± 0.676
0.56ProHis: 0.56 ± 0.211
2.031ProIle: 2.031 ± 0.387
3.081ProLys: 3.081 ± 0.617
3.081ProLeu: 3.081 ± 0.451
1.191ProMet: 1.191 ± 0.28
2.591ProAsn: 2.591 ± 0.486
3.151ProPro: 3.151 ± 0.629
1.821ProGln: 1.821 ± 0.807
2.101ProArg: 2.101 ± 0.416
2.171ProSer: 2.171 ± 0.378
4.062ProThr: 4.062 ± 0.565
3.432ProVal: 3.432 ± 0.456
0.63ProTrp: 0.63 ± 0.181
1.331ProTyr: 1.331 ± 0.369
0.0ProXaa: 0.0 ± 0.0
Gln
4.342GlnAla: 4.342 ± 1.017
0.21GlnCys: 0.21 ± 0.106
1.961GlnAsp: 1.961 ± 0.28
1.191GlnGlu: 1.191 ± 0.347
1.471GlnPhe: 1.471 ± 0.254
3.081GlnGly: 3.081 ± 0.767
0.49GlnHis: 0.49 ± 0.148
2.101GlnIle: 2.101 ± 0.351
1.681GlnLys: 1.681 ± 0.382
3.222GlnLeu: 3.222 ± 0.573
1.261GlnMet: 1.261 ± 0.253
1.611GlnAsn: 1.611 ± 0.336
2.031GlnPro: 2.031 ± 0.948
2.451GlnGln: 2.451 ± 0.828
2.451GlnArg: 2.451 ± 0.602
2.171GlnSer: 2.171 ± 0.407
1.961GlnThr: 1.961 ± 0.4
2.521GlnVal: 2.521 ± 0.514
0.7GlnTrp: 0.7 ± 0.208
0.7GlnTyr: 0.7 ± 0.232
0.0GlnXaa: 0.0 ± 0.0
Arg
5.112ArgAla: 5.112 ± 0.729
0.56ArgCys: 0.56 ± 0.207
3.081ArgAsp: 3.081 ± 0.505
2.871ArgGlu: 2.871 ± 0.598
2.661ArgPhe: 2.661 ± 0.489
3.642ArgGly: 3.642 ± 0.431
0.49ArgHis: 0.49 ± 0.167
3.362ArgIle: 3.362 ± 0.374
3.782ArgLys: 3.782 ± 0.777
4.342ArgLeu: 4.342 ± 0.572
1.331ArgMet: 1.331 ± 0.303
2.871ArgAsn: 2.871 ± 0.377
2.311ArgPro: 2.311 ± 0.419
2.241ArgGln: 2.241 ± 0.343
3.712ArgArg: 3.712 ± 0.649
3.081ArgSer: 3.081 ± 0.468
2.311ArgThr: 2.311 ± 0.484
3.432ArgVal: 3.432 ± 0.488
0.56ArgTrp: 0.56 ± 0.211
1.331ArgTyr: 1.331 ± 0.295
0.0ArgXaa: 0.0 ± 0.0
Ser
6.303SerAla: 6.303 ± 0.627
0.42SerCys: 0.42 ± 0.182
2.031SerAsp: 2.031 ± 0.337
3.642SerGlu: 3.642 ± 0.516
2.521SerPhe: 2.521 ± 0.513
5.463SerGly: 5.463 ± 0.705
0.56SerHis: 0.56 ± 0.199
2.591SerIle: 2.591 ± 0.467
3.502SerLys: 3.502 ± 0.575
4.202SerLeu: 4.202 ± 0.64
1.471SerMet: 1.471 ± 0.277
2.801SerAsn: 2.801 ± 0.38
3.081SerPro: 3.081 ± 0.486
2.731SerGln: 2.731 ± 0.509
3.151SerArg: 3.151 ± 0.304
2.311SerSer: 2.311 ± 0.449
2.521SerThr: 2.521 ± 0.546
3.922SerVal: 3.922 ± 0.484
0.91SerTrp: 0.91 ± 0.251
0.91SerTyr: 0.91 ± 0.206
0.0SerXaa: 0.0 ± 0.0
Thr
7.423ThrAla: 7.423 ± 1.17
0.42ThrCys: 0.42 ± 0.144
3.011ThrAsp: 3.011 ± 0.452
3.782ThrGlu: 3.782 ± 0.638
2.731ThrPhe: 2.731 ± 0.64
5.953ThrGly: 5.953 ± 0.586
0.42ThrHis: 0.42 ± 0.169
2.871ThrIle: 2.871 ± 0.639
2.871ThrLys: 2.871 ± 0.488
4.902ThrLeu: 4.902 ± 0.675
1.191ThrMet: 1.191 ± 0.258
2.661ThrAsn: 2.661 ± 0.482
3.782ThrPro: 3.782 ± 0.618
1.821ThrGln: 1.821 ± 0.44
2.031ThrArg: 2.031 ± 0.326
3.222ThrSer: 3.222 ± 0.674
4.972ThrThr: 4.972 ± 0.705
4.972ThrVal: 4.972 ± 0.842
0.42ThrTrp: 0.42 ± 0.178
1.681ThrTyr: 1.681 ± 0.334
0.0ThrXaa: 0.0 ± 0.0
Val
8.754ValAla: 8.754 ± 1.137
0.77ValCys: 0.77 ± 0.229
3.362ValAsp: 3.362 ± 0.643
5.182ValGlu: 5.182 ± 0.618
3.081ValPhe: 3.081 ± 0.469
5.463ValGly: 5.463 ± 0.555
0.28ValHis: 0.28 ± 0.119
3.502ValIle: 3.502 ± 0.591
3.852ValLys: 3.852 ± 0.626
5.042ValLeu: 5.042 ± 0.721
1.821ValMet: 1.821 ± 0.327
2.521ValAsn: 2.521 ± 0.424
3.151ValPro: 3.151 ± 0.527
1.961ValGln: 1.961 ± 0.446
3.292ValArg: 3.292 ± 0.414
3.922ValSer: 3.922 ± 0.642
4.412ValThr: 4.412 ± 0.936
6.513ValVal: 6.513 ± 0.799
1.121ValTrp: 1.121 ± 0.31
2.241ValTyr: 2.241 ± 0.309
0.0ValXaa: 0.0 ± 0.0
Trp
1.611TrpAla: 1.611 ± 0.295
0.0TrpCys: 0.0 ± 0.0
0.49TrpAsp: 0.49 ± 0.239
0.7TrpGlu: 0.7 ± 0.301
0.63TrpPhe: 0.63 ± 0.243
0.77TrpGly: 0.77 ± 0.286
0.28TrpHis: 0.28 ± 0.144
0.56TrpIle: 0.56 ± 0.221
0.63TrpLys: 0.63 ± 0.293
1.05TrpLeu: 1.05 ± 0.272
0.14TrpMet: 0.14 ± 0.101
0.35TrpAsn: 0.35 ± 0.139
0.7TrpPro: 0.7 ± 0.199
1.541TrpGln: 1.541 ± 0.345
0.84TrpArg: 0.84 ± 0.273
0.28TrpSer: 0.28 ± 0.131
0.14TrpThr: 0.14 ± 0.09
0.84TrpVal: 0.84 ± 0.264
0.07TrpTrp: 0.07 ± 0.06
0.56TrpTyr: 0.56 ± 0.207
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.011TyrAla: 3.011 ± 0.481
0.42TyrCys: 0.42 ± 0.202
2.101TyrAsp: 2.101 ± 0.373
1.681TyrGlu: 1.681 ± 0.331
1.261TyrPhe: 1.261 ± 0.264
2.101TyrGly: 2.101 ± 0.563
0.35TyrHis: 0.35 ± 0.17
1.471TyrIle: 1.471 ± 0.363
0.77TyrLys: 0.77 ± 0.23
1.961TyrLeu: 1.961 ± 0.32
0.98TyrMet: 0.98 ± 0.317
1.261TyrAsn: 1.261 ± 0.33
1.611TyrPro: 1.611 ± 0.464
0.98TyrGln: 0.98 ± 0.301
1.261TyrArg: 1.261 ± 0.232
1.891TyrSer: 1.891 ± 0.441
1.121TyrThr: 1.121 ± 0.28
1.821TyrVal: 1.821 ± 0.356
0.28TyrTrp: 0.28 ± 0.145
0.42TyrTyr: 0.42 ± 0.176
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 61 proteins (14280 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski