Sinirhodobacter populi
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4081 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A443J0A4|A0A443J0A4_9RHOB DUF1441 family protein OS=Sinirhodobacter populi OX=2306993 GN=D2T33_05440 PE=4 SV=1
MM1 pKa = 7.85 TYY3 pKa = 9.81 TPEE6 pKa = 4.25 PFHH9 pKa = 7.88 AEE11 pKa = 4.17 TYY13 pKa = 10.7 DD14 pKa = 3.4 SYY16 pKa = 11.83 QAMLSGLAYY25 pKa = 10.26 AISDD29 pKa = 4.22 SIYY32 pKa = 10.7 KK33 pKa = 10.01 EE34 pKa = 4.09 PTVEE38 pKa = 4.92 APGVWVILTWIEE50 pKa = 3.93 EE51 pKa = 4.2 TDD53 pKa = 3.49 DD54 pKa = 3.75 GGFSVRR60 pKa = 11.84 ASKK63 pKa = 11.02 VGGVPDD69 pKa = 4.02 EE70 pKa = 4.39 EE71 pKa = 4.81 FEE73 pKa = 4.67 DD74 pKa = 3.84 VAEE77 pKa = 4.74 ALDD80 pKa = 3.92 PDD82 pKa = 4.1 FWMTSYY88 pKa = 11.51 DD89 pKa = 3.8 DD90 pKa = 3.93 EE91 pKa = 6.56 RR92 pKa = 11.84 PDD94 pKa = 3.82 TVHH97 pKa = 7.14 HH98 pKa = 7.14 DD99 pKa = 3.94 LVQLLDD105 pKa = 3.83 EE106 pKa = 5.17 RR107 pKa = 11.84 YY108 pKa = 10.35 GDD110 pKa = 3.58 QDD112 pKa = 3.09
Molecular weight: 12.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.732
IPC_protein 3.706
Toseland 3.503
ProMoST 3.821
Dawson 3.706
Bjellqvist 3.897
Wikipedia 3.63
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.037
Thurlkill 3.554
EMBOSS 3.643
Sillero 3.834
Patrickios 0.947
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.772
Protein with the highest isoelectric point:
>tr|A0A443JMD7|A0A443JMD7_9RHOB Protoheme IX farnesyltransferase OS=Sinirhodobacter populi OX=2306993 GN=ctaB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.43 GGRR28 pKa = 11.84 KK29 pKa = 8.99 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.53 GRR39 pKa = 11.84 KK40 pKa = 8.73 VLSAA44 pKa = 4.05
Molecular weight: 5.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4081
0
4081
1300073
38
2877
318.6
34.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.917 ± 0.053
0.844 ± 0.011
5.709 ± 0.028
5.611 ± 0.034
3.635 ± 0.023
8.94 ± 0.035
1.995 ± 0.019
5.215 ± 0.026
2.822 ± 0.033
10.262 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.662 ± 0.016
2.374 ± 0.02
5.458 ± 0.031
3.006 ± 0.022
7.37 ± 0.039
4.904 ± 0.03
5.391 ± 0.026
7.337 ± 0.03
1.411 ± 0.016
2.135 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here