Streptococcus satellite phage Javan726
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZTX0|A0A4D5ZTX0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan726 OX=2558820 GN=JavanS726_0018 PE=4 SV=1
MM1 pKa = 7.22 TLDD4 pKa = 4.01 LGKK7 pKa = 8.39 MTQAEE12 pKa = 4.45 FDD14 pKa = 3.72 EE15 pKa = 4.66 VMADD19 pKa = 3.21 IKK21 pKa = 11.13 ARR23 pKa = 11.84 NPNLLQLITDD33 pKa = 4.52 FLDD36 pKa = 3.62 RR37 pKa = 11.84 KK38 pKa = 8.24 VTPEE42 pKa = 4.05 EE43 pKa = 3.86 VDD45 pKa = 3.54 DD46 pKa = 4.24
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.494
IPC2_protein 4.113
IPC_protein 3.986
Toseland 3.783
ProMoST 4.05
Dawson 3.986
Bjellqvist 4.228
Wikipedia 3.935
Rodwell 3.821
Grimsley 3.706
Solomon 3.961
Lehninger 3.923
Nozaki 4.113
DTASelect 4.329
Thurlkill 3.859
EMBOSS 3.948
Sillero 4.113
Patrickios 4.202
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 4.054
Protein with the highest isoelectric point:
>tr|A0A4D5ZUC1|A0A4D5ZUC1_9VIRU Integrase OS=Streptococcus satellite phage Javan726 OX=2558820 GN=JavanS726_0001 PE=3 SV=1
MM1 pKa = 7.12 MPKK4 pKa = 10.19 SNYY7 pKa = 9.53 RR8 pKa = 11.84 EE9 pKa = 3.74 MRR11 pKa = 11.84 FISSLGEE18 pKa = 3.68 KK19 pKa = 10.38 LLATRR24 pKa = 11.84 ILFTVTIANNQQ35 pKa = 3.18
Molecular weight: 4.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.255
IPC2_protein 9.619
IPC_protein 10.145
Toseland 10.482
ProMoST 10.101
Dawson 10.613
Bjellqvist 10.262
Wikipedia 10.774
Rodwell 10.965
Grimsley 10.657
Solomon 10.701
Lehninger 10.687
Nozaki 10.438
DTASelect 10.262
Thurlkill 10.482
EMBOSS 10.877
Sillero 10.526
Patrickios 10.906
IPC_peptide 10.701
IPC2_peptide 8.931
IPC2.peptide.svr19 8.647
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20
0
20
3213
35
484
160.7
18.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.918 ± 0.362
0.778 ± 0.148
6.256 ± 0.564
8.901 ± 0.626
4.606 ± 0.463
4.7 ± 0.4
1.556 ± 0.26
6.723 ± 0.492
9.773 ± 0.584
10.24 ± 0.592
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.334 ± 0.266
5.571 ± 0.315
2.552 ± 0.239
3.268 ± 0.422
5.602 ± 0.424
5.54 ± 0.48
5.727 ± 0.425
5.727 ± 0.434
0.654 ± 0.124
4.575 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here