Oryza meyeriana var. granulata
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44256 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G1CRB6|A0A6G1CRB6_9ORYZ Uncharacterized protein OS=Oryza meyeriana var. granulata OX=110450 GN=E2562_018278 PE=3 SV=1
MM1 pKa = 8.22 DD2 pKa = 6.58 DD3 pKa = 5.39 DD4 pKa = 5.56 LVHH7 pKa = 6.82 DD8 pKa = 4.98 VNWALAPDD16 pKa = 3.85 SCDD19 pKa = 3.95 DD20 pKa = 4.2 QDD22 pKa = 4.0 GQADD26 pKa = 3.49 AVAEE30 pKa = 4.3 SEE32 pKa = 4.16 RR33 pKa = 11.84 QEE35 pKa = 4.45 ADD37 pKa = 3.17 VVMGDD42 pKa = 3.07 SDD44 pKa = 4.11 VVHH47 pKa = 6.34 FVVDD51 pKa = 3.84 SMAGDD56 pKa = 3.47 IEE58 pKa = 4.9 VPDD61 pKa = 3.89 LQVAVDD67 pKa = 3.58 IKK69 pKa = 11.07 VDD71 pKa = 3.85 NVSYY75 pKa = 9.67 WCDD78 pKa = 2.81 RR79 pKa = 11.84 CGRR82 pKa = 11.84 VHH84 pKa = 7.51 GDD86 pKa = 3.18 GWWRR90 pKa = 11.84 SCKK93 pKa = 10.31 GPCSRR98 pKa = 11.84 CGLVRR103 pKa = 11.84 RR104 pKa = 11.84 DD105 pKa = 3.96 YY106 pKa = 11.25 MPMSWIYY113 pKa = 11.05 GLDD116 pKa = 3.4 EE117 pKa = 5.13 FDD119 pKa = 4.33 YY120 pKa = 11.13 EE121 pKa = 5.39 SNNNYY126 pKa = 8.75 QAQFQNN132 pKa = 3.63
Molecular weight: 14.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.834
IPC_protein 3.846
Toseland 3.617
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.834
Rodwell 3.681
Grimsley 3.528
Solomon 3.859
Lehninger 3.808
Nozaki 3.973
DTASelect 4.279
Thurlkill 3.681
EMBOSS 3.834
Sillero 3.973
Patrickios 1.1
IPC_peptide 3.846
IPC2_peptide 3.948
IPC2.peptide.svr19 3.87
Protein with the highest isoelectric point:
>tr|A0A6G1D920|A0A6G1D920_9ORYZ Uncharacterized protein OS=Oryza meyeriana var. granulata OX=110450 GN=E2562_032957 PE=4 SV=1
MM1 pKa = 7.98 AMGQRR6 pKa = 11.84 QRR8 pKa = 11.84 GRR10 pKa = 11.84 GGGVGVGIGAVRR22 pKa = 11.84 ARR24 pKa = 11.84 ARR26 pKa = 11.84 WRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 VPGVGVGNGAPLARR44 pKa = 11.84 MAAAATTLGRR54 pKa = 11.84 RR55 pKa = 11.84 GPARR59 pKa = 11.84 TRR61 pKa = 11.84 LRR63 pKa = 11.84 QSRR66 pKa = 11.84 GRR68 pKa = 11.84 AVV70 pKa = 2.82
Molecular weight: 7.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.433
IPC_protein 13.042
Toseland 13.203
ProMoST 13.7
Dawson 13.203
Bjellqvist 13.203
Wikipedia 13.685
Rodwell 12.705
Grimsley 13.247
Solomon 13.7
Lehninger 13.598
Nozaki 13.203
DTASelect 13.203
Thurlkill 13.203
EMBOSS 13.7
Sillero 13.203
Patrickios 12.427
IPC_peptide 13.7
IPC2_peptide 12.691
IPC2.peptide.svr19 9.338
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
39309
4947
44256
13918302
49
5155
314.5
34.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.439 ± 0.019
1.897 ± 0.007
5.387 ± 0.011
6.077 ± 0.016
3.558 ± 0.008
7.5 ± 0.013
2.473 ± 0.006
4.263 ± 0.009
4.862 ± 0.011
9.355 ± 0.015
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.473 ± 0.005
3.394 ± 0.009
5.61 ± 0.014
3.445 ± 0.009
6.467 ± 0.011
8.259 ± 0.014
4.832 ± 0.008
6.846 ± 0.009
1.336 ± 0.005
2.522 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here