Rhodococcus sp. S2-17

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; unclassified Rhodococcus

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6874 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S2C5L2|A0A2S2C5L2_9NOCA Uncharacterized protein OS=Rhodococcus sp. S2-17 OX=1990687 GN=CBI38_32340 PE=4 SV=1
MM1 pKa = 7.4AVVKK5 pKa = 10.63ANLGACQGYY14 pKa = 10.24ANCVVAADD22 pKa = 4.88DD23 pKa = 4.68YY24 pKa = 11.91FDD26 pKa = 5.01IDD28 pKa = 3.94DD29 pKa = 4.94DD30 pKa = 4.74GVVEE34 pKa = 4.16LLSVEE39 pKa = 4.28VPEE42 pKa = 4.39SEE44 pKa = 4.3RR45 pKa = 11.84GRR47 pKa = 11.84VDD49 pKa = 3.01SAARR53 pKa = 11.84SCPVSALWIEE63 pKa = 4.39DD64 pKa = 3.3

Molecular weight: 6.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S2BSA5|A0A2S2BSA5_9NOCA Glycosyl transferase OS=Rhodococcus sp. S2-17 OX=1990687 GN=CBI38_07565 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6874

0

6874

2188525

24

11272

318.4

34.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.492 ± 0.04

0.816 ± 0.008

6.277 ± 0.023

5.654 ± 0.027

3.033 ± 0.018

8.892 ± 0.03

2.266 ± 0.015

4.277 ± 0.02

2.18 ± 0.024

9.937 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.973 ± 0.012

2.113 ± 0.015

5.559 ± 0.023

2.836 ± 0.016

7.279 ± 0.027

5.832 ± 0.022

6.296 ± 0.019

8.823 ± 0.027

1.429 ± 0.013

2.037 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski