Corynebacterium sp. LMM-1652
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2031 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5J6Z715|A0A5J6Z715_9CORY Mrr_cat domain-containing protein OS=Corynebacterium sp. LMM-1652 OX=2487892 GN=CUROG_00295 PE=4 SV=1
MM1 pKa = 7.04 NRR3 pKa = 11.84 AAIAAAALALALAGCSATDD22 pKa = 3.95 PEE24 pKa = 4.45 PTADD28 pKa = 3.43 GTVSQNTFLTTHH40 pKa = 6.82 GLAAMDD46 pKa = 3.5 AVEE49 pKa = 5.81 IIDD52 pKa = 3.65 HH53 pKa = 7.28 LDD55 pKa = 3.38 RR56 pKa = 11.84 QKK58 pKa = 9.9 VTEE61 pKa = 4.56 RR62 pKa = 11.84 PTDD65 pKa = 4.58 LIASVRR71 pKa = 11.84 ADD73 pKa = 3.78 EE74 pKa = 5.15 LLLSSDD80 pKa = 3.72 DD81 pKa = 3.78 QEE83 pKa = 4.76 VVVDD87 pKa = 4.99 LPDD90 pKa = 3.5 NQTYY94 pKa = 10.58 VSIAPYY100 pKa = 8.84 LTSTHH105 pKa = 6.73 DD106 pKa = 3.63 CFYY109 pKa = 11.19 HH110 pKa = 7.34 SLTTCLGEE118 pKa = 5.12 LDD120 pKa = 4.42 NEE122 pKa = 4.93 DD123 pKa = 3.46 IQVTITDD130 pKa = 3.75 EE131 pKa = 4.08 ATGEE135 pKa = 4.27 VLVDD139 pKa = 3.43 EE140 pKa = 4.91 ATTTFDD146 pKa = 3.45 NGFIGFWLPDD156 pKa = 3.74 DD157 pKa = 3.7 VTGLIEE163 pKa = 3.76 VSYY166 pKa = 10.43 QGRR169 pKa = 11.84 TGTTEE174 pKa = 4.16 FSTADD179 pKa = 3.59 DD180 pKa = 4.31 GATCVTDD187 pKa = 3.36 LHH189 pKa = 6.16 LTT191 pKa = 3.66
Molecular weight: 20.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.706
IPC_protein 3.719
Toseland 3.49
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 4.088
Thurlkill 3.554
EMBOSS 3.668
Sillero 3.846
Patrickios 1.062
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.761
Protein with the highest isoelectric point:
>tr|A0A5J6Z395|A0A5J6Z395_9CORY Nitric oxide dioxygenase OS=Corynebacterium sp. LMM-1652 OX=2487892 GN=hmp PE=3 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.97 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MSTRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVSARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.22 GRR42 pKa = 11.84 KK43 pKa = 9.07 SLTAA47 pKa = 4.07
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2031
0
2031
687179
29
1665
338.3
36.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.426 ± 0.067
0.703 ± 0.012
5.905 ± 0.046
6.674 ± 0.055
3.207 ± 0.031
8.595 ± 0.045
2.168 ± 0.027
4.842 ± 0.035
3.431 ± 0.046
9.253 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.353 ± 0.023
2.946 ± 0.031
5.023 ± 0.044
3.5 ± 0.031
6.254 ± 0.05
6.067 ± 0.041
5.972 ± 0.04
8.149 ± 0.045
1.403 ± 0.021
2.13 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here