Synechococcus sp. (strain CC9902)
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2301 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q3AZM1|ATPA_SYNS9 ATP synthase subunit alpha OS=Synechococcus sp. (strain CC9902) OX=316279 GN=atpA PE=3 SV=1
MM1 pKa = 6.98 TFEE4 pKa = 4.94 VLFDD8 pKa = 5.26 DD9 pKa = 5.44 GNQSIPVEE17 pKa = 4.05 RR18 pKa = 11.84 FEE20 pKa = 4.44 TLGDD24 pKa = 4.61 AIACYY29 pKa = 10.16 VSCIINANEE38 pKa = 3.62 GMNAVEE44 pKa = 4.79 IVDD47 pKa = 5.35 DD48 pKa = 4.13 YY49 pKa = 11.87 FEE51 pKa = 5.16 TIASHH56 pKa = 6.67 SFADD60 pKa = 6.0 FINEE64 pKa = 3.99 KK65 pKa = 10.66 QNN67 pKa = 3.23
Molecular weight: 7.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.745
IPC_protein 3.617
Toseland 3.452
ProMoST 3.808
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.567
Rodwell 3.478
Grimsley 3.376
Solomon 3.579
Lehninger 3.528
Nozaki 3.77
DTASelect 3.897
Thurlkill 3.516
EMBOSS 3.567
Sillero 3.745
Patrickios 0.604
IPC_peptide 3.579
IPC2_peptide 3.719
IPC2.peptide.svr19 3.731
Protein with the highest isoelectric point:
>sp|Q3AV44|PNP_SYNS9 Polyribonucleotide nucleotidyltransferase OS=Synechococcus sp. (strain CC9902) OX=316279 GN=pnp PE=3 SV=1
MM1 pKa = 7.55 TKK3 pKa = 9.01 RR4 pKa = 11.84 TLGGTSRR11 pKa = 11.84 KK12 pKa = 9.07 RR13 pKa = 11.84 KK14 pKa = 8.17 RR15 pKa = 11.84 VSGFRR20 pKa = 11.84 VRR22 pKa = 11.84 MRR24 pKa = 11.84 SHH26 pKa = 6.21 TGRR29 pKa = 11.84 RR30 pKa = 11.84 VIRR33 pKa = 11.84 TRR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.21 RR38 pKa = 11.84 GRR40 pKa = 11.84 TRR42 pKa = 11.84 LAVV45 pKa = 3.28
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.389
IPC_protein 12.983
Toseland 13.144
ProMoST 13.642
Dawson 13.144
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.749
Grimsley 13.188
Solomon 13.642
Lehninger 13.554
Nozaki 13.144
DTASelect 13.144
Thurlkill 13.144
EMBOSS 13.642
Sillero 13.144
Patrickios 12.486
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.284
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2301
0
2301
664488
25
3504
288.8
31.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.753 ± 0.058
1.268 ± 0.018
5.537 ± 0.044
5.711 ± 0.051
3.236 ± 0.032
8.066 ± 0.058
2.18 ± 0.026
4.687 ± 0.05
3.116 ± 0.046
12.179 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.134 ± 0.021
3.02 ± 0.041
5.325 ± 0.046
4.74 ± 0.04
6.921 ± 0.052
6.608 ± 0.048
4.929 ± 0.038
6.974 ± 0.044
1.735 ± 0.029
1.88 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here