Amino acid dipepetide frequency for Synechococcus sp. (strain CC9902)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.105AlaAla: 11.105 ± 0.168	1.395AlaCys: 1.395 ± 0.051	5.544AlaAsp: 5.544 ± 0.098	5.872AlaGlu: 5.872 ± 0.113	3.082AlaPhe: 3.082 ± 0.08	7.868AlaGly: 7.868 ± 0.114	1.673AlaHis: 1.673 ± 0.057	5.15AlaIle: 5.15 ± 0.108	2.968AlaLys: 2.968 ± 0.067	12.518AlaLeu: 12.518 ± 0.18	2.787AlaMet: 2.787 ± 0.067	2.564AlaAsn: 2.564 ± 0.071	4.328AlaPro: 4.328 ± 0.099	3.765AlaGln: 3.765 ± 0.081	5.383AlaArg: 5.383 ± 0.098	6.197AlaSer: 6.197 ± 0.098	4.601AlaThr: 4.601 ± 0.083	7.377AlaVal: 7.377 ± 0.122	1.609AlaTrp: 1.609 ± 0.047	1.744AlaTyr: 1.744 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.959CysAla: 0.959 ± 0.045	0.277CysCys: 0.277 ± 0.023	0.746CysAsp: 0.746 ± 0.039	0.644CysGlu: 0.644 ± 0.038	0.483CysPhe: 0.483 ± 0.031	1.23CysGly: 1.23 ± 0.048	0.355CysHis: 0.355 ± 0.025	0.567CysIle: 0.567 ± 0.03	0.289CysLys: 0.289 ± 0.022	1.439CysLeu: 1.439 ± 0.046	0.19CysMet: 0.19 ± 0.017	0.34CysAsn: 0.34 ± 0.023	0.73CysPro: 0.73 ± 0.037	0.476CysGln: 0.476 ± 0.023	1.065CysArg: 1.065 ± 0.044	1.035CysSer: 1.035 ± 0.043	0.609CysThr: 0.609 ± 0.029	0.727CysVal: 0.727 ± 0.035	0.293CysTrp: 0.293 ± 0.022	0.226CysTyr: 0.226 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	5.382AspAla: 5.382 ± 0.106	0.649AspCys: 0.649 ± 0.032	3.13AspAsp: 3.13 ± 0.073	3.005AspGlu: 3.005 ± 0.075	1.774AspPhe: 1.774 ± 0.055	4.774AspGly: 4.774 ± 0.101	1.311AspHis: 1.311 ± 0.063	1.97AspIle: 1.97 ± 0.058	1.285AspLys: 1.285 ± 0.05	7.57AspLeu: 7.57 ± 0.112	0.868AspMet: 0.868 ± 0.037	1.338AspAsn: 1.338 ± 0.053	3.838AspPro: 3.838 ± 0.088	3.32AspGln: 3.32 ± 0.078	3.962AspArg: 3.962 ± 0.086	2.963AspSer: 2.963 ± 0.078	2.113AspThr: 2.113 ± 0.071	3.824AspVal: 3.824 ± 0.087	1.12AspTrp: 1.12 ± 0.046	1.177AspTyr: 1.177 ± 0.052	0.0AspXaa: 0.0 ± 0.0
Glu	6.698GluAla: 6.698 ± 0.134	0.482GluCys: 0.482 ± 0.024	2.512GluAsp: 2.512 ± 0.07	3.273GluGlu: 3.273 ± 0.09	1.445GluPhe: 1.445 ± 0.046	4.268GluGly: 4.268 ± 0.091	1.243GluHis: 1.243 ± 0.048	2.73GluIle: 2.73 ± 0.076	1.878GluLys: 1.878 ± 0.066	7.508GluLeu: 7.508 ± 0.106	1.205GluMet: 1.205 ± 0.045	1.362GluAsn: 1.362 ± 0.049	2.799GluPro: 2.799 ± 0.073	3.564GluGln: 3.564 ± 0.085	4.856GluArg: 4.856 ± 0.098	3.344GluSer: 3.344 ± 0.077	2.762GluThr: 2.762 ± 0.062	3.654GluVal: 3.654 ± 0.083	0.748GluTrp: 0.748 ± 0.037	0.783GluTyr: 0.783 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	2.929PheAla: 2.929 ± 0.06	0.537PheCys: 0.537 ± 0.03	2.227PheAsp: 2.227 ± 0.06	1.956PheGlu: 1.956 ± 0.057	1.16PhePhe: 1.16 ± 0.044	2.96PheGly: 2.96 ± 0.075	0.679PheHis: 0.679 ± 0.029	1.383PheIle: 1.383 ± 0.051	1.026PheLys: 1.026 ± 0.034	3.299PheLeu: 3.299 ± 0.085	0.584PheMet: 0.584 ± 0.029	1.264PheAsn: 1.264 ± 0.051	1.509PhePro: 1.509 ± 0.047	1.415PheGln: 1.415 ± 0.047	2.048PheArg: 2.048 ± 0.055	2.417PheSer: 2.417 ± 0.065	1.683PheThr: 1.683 ± 0.051	1.956PheVal: 1.956 ± 0.051	0.554PheTrp: 0.554 ± 0.032	0.772PheTyr: 0.772 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	6.909GlyAla: 6.909 ± 0.126	1.314GlyCys: 1.314 ± 0.044	4.587GlyAsp: 4.587 ± 0.09	4.467GlyGlu: 4.467 ± 0.088	3.288GlyPhe: 3.288 ± 0.076	6.608GlyGly: 6.608 ± 0.175	1.779GlyHis: 1.779 ± 0.051	4.161GlyIle: 4.161 ± 0.087	2.465GlyLys: 2.465 ± 0.082	10.191GlyLeu: 10.191 ± 0.151	1.941GlyMet: 1.941 ± 0.061	2.113GlyAsn: 2.113 ± 0.077	3.187GlyPro: 3.187 ± 0.07	3.315GlyGln: 3.315 ± 0.062	5.541GlyArg: 5.541 ± 0.101	5.293GlySer: 5.293 ± 0.092	4.027GlyThr: 4.027 ± 0.084	5.88GlyVal: 5.88 ± 0.107	1.705GlyTrp: 1.705 ± 0.059	1.892GlyTyr: 1.892 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	1.753HisAla: 1.753 ± 0.059	0.405HisCys: 0.405 ± 0.023	1.058HisAsp: 1.058 ± 0.048	0.861HisGlu: 0.861 ± 0.036	0.771HisPhe: 0.771 ± 0.038	1.95HisGly: 1.95 ± 0.061	0.847HisHis: 0.847 ± 0.043	0.793HisIle: 0.793 ± 0.036	0.504HisLys: 0.504 ± 0.029	2.641HisLeu: 2.641 ± 0.074	0.298HisMet: 0.298 ± 0.025	0.673HisAsn: 0.673 ± 0.029	1.663HisPro: 1.663 ± 0.057	1.258HisGln: 1.258 ± 0.044	1.875HisArg: 1.875 ± 0.062	1.294HisSer: 1.294 ± 0.046	0.871HisThr: 0.871 ± 0.039	1.145HisVal: 1.145 ± 0.041	0.637HisTrp: 0.637 ± 0.036	0.504HisTyr: 0.504 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	4.924IleAla: 4.924 ± 0.098	0.593IleCys: 0.593 ± 0.034	3.342IleAsp: 3.342 ± 0.079	3.172IleGlu: 3.172 ± 0.078	1.243IlePhe: 1.243 ± 0.051	4.421IleGly: 4.421 ± 0.089	1.141IleHis: 1.141 ± 0.043	1.457IleIle: 1.457 ± 0.055	1.661IleLys: 1.661 ± 0.06	3.782IleLeu: 3.782 ± 0.088	0.464IleMet: 0.464 ± 0.03	1.871IleAsn: 1.871 ± 0.059	2.746IlePro: 2.746 ± 0.063	2.101IleGln: 2.101 ± 0.057	3.15IleArg: 3.15 ± 0.06	3.434IleSer: 3.434 ± 0.085	2.923IleThr: 2.923 ± 0.076	2.795IleVal: 2.795 ± 0.073	0.688IleTrp: 0.688 ± 0.037	0.959IleTyr: 0.959 ± 0.045	0.0IleXaa: 0.0 ± 0.0
Lys	3.526LysAla: 3.526 ± 0.083	0.232LysCys: 0.232 ± 0.021	1.735LysAsp: 1.735 ± 0.063	1.801LysGlu: 1.801 ± 0.061	0.766LysPhe: 0.766 ± 0.034	2.54LysGly: 2.54 ± 0.074	0.567LysHis: 0.567 ± 0.032	1.41LysIle: 1.41 ± 0.055	1.342LysLys: 1.342 ± 0.058	3.181LysLeu: 3.181 ± 0.07	0.467LysMet: 0.467 ± 0.024	1.019LysAsn: 1.019 ± 0.044	1.887LysPro: 1.887 ± 0.061	1.616LysGln: 1.616 ± 0.05	2.408LysArg: 2.408 ± 0.068	1.934LysSer: 1.934 ± 0.062	1.756LysThr: 1.756 ± 0.055	2.116LysVal: 2.116 ± 0.062	0.271LysTrp: 0.271 ± 0.02	0.588LysTyr: 0.588 ± 0.031	0.0LysXaa: 0.0 ± 0.0
Leu	12.095LeuAla: 12.095 ± 0.165	1.38LeuCys: 1.38 ± 0.052	6.653LeuAsp: 6.653 ± 0.104	7.811LeuGlu: 7.811 ± 0.111	3.497LeuPhe: 3.497 ± 0.081	9.093LeuGly: 9.093 ± 0.129	2.604LeuHis: 2.604 ± 0.062	6.257LeuIle: 6.257 ± 0.122	4.223LeuLys: 4.223 ± 0.08	14.732LeuLeu: 14.732 ± 0.258	2.796LeuMet: 2.796 ± 0.074	3.976LeuAsn: 3.976 ± 0.075	6.667LeuPro: 6.667 ± 0.106	6.184LeuGln: 6.184 ± 0.114	8.039LeuArg: 8.039 ± 0.119	7.925LeuSer: 7.925 ± 0.129	5.651LeuThr: 5.651 ± 0.097	8.704LeuVal: 8.704 ± 0.127	1.702LeuTrp: 1.702 ± 0.064	1.8LeuTyr: 1.8 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	2.977MetAla: 2.977 ± 0.075	0.14MetCys: 0.14 ± 0.014	1.115MetAsp: 1.115 ± 0.042	1.275MetGlu: 1.275 ± 0.046	0.451MetPhe: 0.451 ± 0.026	1.883MetGly: 1.883 ± 0.052	0.418MetHis: 0.418 ± 0.025	0.793MetIle: 0.793 ± 0.04	0.755MetLys: 0.755 ± 0.034	2.128MetLeu: 2.128 ± 0.059	0.352MetMet: 0.352 ± 0.024	0.674MetAsn: 0.674 ± 0.031	1.272MetPro: 1.272 ± 0.043	0.888MetGln: 0.888 ± 0.037	1.362MetArg: 1.362 ± 0.048	1.457MetSer: 1.457 ± 0.049	1.332MetThr: 1.332 ± 0.052	1.666MetVal: 1.666 ± 0.049	0.157MetTrp: 0.157 ± 0.015	0.245MetTyr: 0.245 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.813AsnAla: 2.813 ± 0.073	0.369AsnCys: 0.369 ± 0.022	1.707AsnAsp: 1.707 ± 0.066	1.317AsnGlu: 1.317 ± 0.054	1.025AsnPhe: 1.025 ± 0.044	2.558AsnGly: 2.558 ± 0.07	0.737AsnHis: 0.737 ± 0.04	1.279AsnIle: 1.279 ± 0.047	1.014AsnLys: 1.014 ± 0.04	3.472AsnLeu: 3.472 ± 0.08	0.464AsnMet: 0.464 ± 0.03	1.156AsnAsn: 1.156 ± 0.051	2.119AsnPro: 2.119 ± 0.06	1.526AsnGln: 1.526 ± 0.061	2.313AsnArg: 2.313 ± 0.064	1.97AsnSer: 1.97 ± 0.067	1.442AsnThr: 1.442 ± 0.054	1.713AsnVal: 1.713 ± 0.046	0.53AsnTrp: 0.53 ± 0.029	0.676AsnTyr: 0.676 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	4.762ProAla: 4.762 ± 0.106	0.551ProCys: 0.551 ± 0.028	3.195ProAsp: 3.195 ± 0.076	3.723ProGlu: 3.723 ± 0.069	1.828ProPhe: 1.828 ± 0.058	4.203ProGly: 4.203 ± 0.091	1.05ProHis: 1.05 ± 0.047	2.53ProIle: 2.53 ± 0.059	1.692ProLys: 1.692 ± 0.054	6.476ProLeu: 6.476 ± 0.126	1.278ProMet: 1.278 ± 0.044	1.664ProAsn: 1.664 ± 0.047	2.455ProPro: 2.455 ± 0.07	2.277ProGln: 2.277 ± 0.062	2.837ProArg: 2.837 ± 0.072	3.857ProSer: 3.857 ± 0.081	2.798ProThr: 2.798 ± 0.061	3.988ProVal: 3.988 ± 0.091	1.056ProTrp: 1.056 ± 0.04	1.032ProTyr: 1.032 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	4.792GlnAla: 4.792 ± 0.102	0.451GlnCys: 0.451 ± 0.025	1.99GlnAsp: 1.99 ± 0.061	2.5GlnGlu: 2.5 ± 0.066	1.227GlnPhe: 1.227 ± 0.043	3.41GlnGly: 3.41 ± 0.074	1.144GlnHis: 1.144 ± 0.041	1.996GlnIle: 1.996 ± 0.053	1.446GlnLys: 1.446 ± 0.046	6.707GlnLeu: 6.707 ± 0.131	0.913GlnMet: 0.913 ± 0.039	1.201GlnAsn: 1.201 ± 0.043	2.746GlnPro: 2.746 ± 0.069	3.084GlnGln: 3.084 ± 0.087	4.394GlnArg: 4.394 ± 0.105	2.796GlnSer: 2.796 ± 0.07	1.997GlnThr: 1.997 ± 0.058	3.208GlnVal: 3.208 ± 0.078	0.85GlnTrp: 0.85 ± 0.043	0.552GlnTyr: 0.552 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	4.998ArgAla: 4.998 ± 0.097	1.059ArgCys: 1.059 ± 0.044	3.669ArgAsp: 3.669 ± 0.085	3.558ArgGlu: 3.558 ± 0.075	2.846ArgPhe: 2.846 ± 0.061	4.635ArgGly: 4.635 ± 0.097	1.726ArgHis: 1.726 ± 0.048	3.348ArgIle: 3.348 ± 0.071	1.955ArgLys: 1.955 ± 0.058	9.251ArgLeu: 9.251 ± 0.138	1.583ArgMet: 1.583 ± 0.046	1.997ArgAsn: 1.997 ± 0.058	3.464ArgPro: 3.464 ± 0.076	3.624ArgGln: 3.624 ± 0.084	5.967ArgArg: 5.967 ± 0.114	5.01ArgSer: 5.01 ± 0.111	2.927ArgThr: 2.927 ± 0.073	4.247ArgVal: 4.247 ± 0.081	1.725ArgTrp: 1.725 ± 0.053	1.616ArgTyr: 1.616 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	5.824SerAla: 5.824 ± 0.099	0.903SerCys: 0.903 ± 0.038	3.794SerAsp: 3.794 ± 0.086	3.452SerGlu: 3.452 ± 0.074	2.358SerPhe: 2.358 ± 0.063	5.689SerGly: 5.689 ± 0.105	1.446SerHis: 1.446 ± 0.054	3.278SerIle: 3.278 ± 0.08	2.056SerLys: 2.056 ± 0.072	7.583SerLeu: 7.583 ± 0.126	1.609SerMet: 1.609 ± 0.049	2.14SerAsn: 2.14 ± 0.061	3.296SerPro: 3.296 ± 0.08	2.594SerGln: 2.594 ± 0.059	4.39SerArg: 4.39 ± 0.092	4.993SerSer: 4.993 ± 0.119	3.744SerThr: 3.744 ± 0.096	4.288SerVal: 4.288 ± 0.082	1.29SerTrp: 1.29 ± 0.048	1.351SerTyr: 1.351 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	5.243ThrAla: 5.243 ± 0.095	0.554ThrCys: 0.554 ± 0.029	2.503ThrAsp: 2.503 ± 0.064	2.476ThrGlu: 2.476 ± 0.064	1.514ThrPhe: 1.514 ± 0.052	4.166ThrGly: 4.166 ± 0.09	0.953ThrHis: 0.953 ± 0.036	2.445ThrIle: 2.445 ± 0.063	1.436ThrLys: 1.436 ± 0.048	5.681ThrLeu: 5.681 ± 0.1	0.975ThrMet: 0.975 ± 0.036	1.547ThrAsn: 1.547 ± 0.057	3.356ThrPro: 3.356 ± 0.077	1.74ThrGln: 1.74 ± 0.05	2.715ThrArg: 2.715 ± 0.071	3.323ThrSer: 3.323 ± 0.08	3.144ThrThr: 3.144 ± 0.075	3.692ThrVal: 3.692 ± 0.082	0.748ThrTrp: 0.748 ± 0.036	1.084ThrTyr: 1.084 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	6.896ValAla: 6.896 ± 0.125	0.783ValCys: 0.783 ± 0.035	4.065ValAsp: 4.065 ± 0.076	4.37ValGlu: 4.37 ± 0.086	2.304ValPhe: 2.304 ± 0.062	5.279ValGly: 5.279 ± 0.096	1.366ValHis: 1.366 ± 0.048	3.674ValIle: 3.674 ± 0.076	2.036ValLys: 2.036 ± 0.06	8.896ValLeu: 8.896 ± 0.147	1.743ValMet: 1.743 ± 0.053	2.148ValAsn: 2.148 ± 0.052	3.285ValPro: 3.285 ± 0.075	2.844ValGln: 2.844 ± 0.064	4.038ValArg: 4.038 ± 0.085	4.217ValSer: 4.217 ± 0.079	3.269ValThr: 3.269 ± 0.085	6.537ValVal: 6.537 ± 0.113	0.901ValTrp: 0.901 ± 0.035	1.093ValTyr: 1.093 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.267TrpAla: 1.267 ± 0.041	0.284TrpCys: 0.284 ± 0.023	0.752TrpAsp: 0.752 ± 0.033	0.614TrpGlu: 0.614 ± 0.036	0.634TrpPhe: 0.634 ± 0.033	1.183TrpGly: 1.183 ± 0.05	0.388TrpHis: 0.388 ± 0.025	0.935TrpIle: 0.935 ± 0.042	0.53TrpLys: 0.53 ± 0.029	2.585TrpLeu: 2.585 ± 0.077	0.522TrpMet: 0.522 ± 0.029	0.542TrpAsn: 0.542 ± 0.03	0.921TrpPro: 0.921 ± 0.043	0.956TrpGln: 0.956 ± 0.04	1.377TrpArg: 1.377 ± 0.048	1.287TrpSer: 1.287 ± 0.046	0.85TrpThr: 0.85 ± 0.04	1.028TrpVal: 1.028 ± 0.044	0.414TrpTrp: 0.414 ± 0.029	0.281TrpTyr: 0.281 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.683TyrAla: 1.683 ± 0.052	0.324TyrCys: 0.324 ± 0.022	1.047TyrAsp: 1.047 ± 0.045	0.966TyrGlu: 0.966 ± 0.039	0.662TyrPhe: 0.662 ± 0.032	1.946TyrGly: 1.946 ± 0.063	0.373TyrHis: 0.373 ± 0.023	0.71TyrIle: 0.71 ± 0.033	0.602TyrLys: 0.602 ± 0.034	2.149TyrLeu: 2.149 ± 0.067	0.299TyrMet: 0.299 ± 0.02	0.609TyrAsn: 0.609 ± 0.04	0.983TyrPro: 0.983 ± 0.037	0.859TyrGln: 0.859 ± 0.039	1.484TyrArg: 1.484 ± 0.048	1.332TyrSer: 1.332 ± 0.046	0.795TyrThr: 0.795 ± 0.033	1.198TyrVal: 1.198 ± 0.041	0.354TyrTrp: 0.354 ± 0.023	0.423TyrTyr: 0.423 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2301 proteins (664488 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---