Rhizobium etli CNPAF512

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; Rhizobium etli

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6543 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2A6N4|F2A6N4_RHIET Histidine kinase OS=Rhizobium etli CNPAF512 OX=993047 GN=RHECNPAF_180008 PE=4 SV=1
MM1 pKa = 7.69ANHH4 pKa = 6.66KK5 pKa = 9.99PIAADD10 pKa = 3.84VYY12 pKa = 11.15QPIFDD17 pKa = 3.54IDD19 pKa = 3.77KK20 pKa = 10.39PVDD23 pKa = 3.55GNILDD28 pKa = 4.37GSTDD32 pKa = 3.55ADD34 pKa = 3.76GDD36 pKa = 3.97PLRR39 pKa = 11.84LNFVNGQRR47 pKa = 11.84IPQPANPDD55 pKa = 3.27GPATTTAIEE64 pKa = 4.35GKK66 pKa = 10.33YY67 pKa = 9.07GTLTIYY73 pKa = 10.95SDD75 pKa = 3.72GYY77 pKa = 8.09YY78 pKa = 9.96TYY80 pKa = 10.84EE81 pKa = 4.63LDD83 pKa = 3.24HH84 pKa = 6.82SNPVVSALGPGDD96 pKa = 3.64QLVDD100 pKa = 3.26QFNFKK105 pKa = 10.45ISDD108 pKa = 3.65GKK110 pKa = 10.95GATDD114 pKa = 4.75FGLLNIAVDD123 pKa = 4.0LPEE126 pKa = 6.29RR127 pKa = 11.84GDD129 pKa = 3.2IFVNFEE135 pKa = 4.32DD136 pKa = 4.01VGKK139 pKa = 10.07HH140 pKa = 5.98DD141 pKa = 4.69FPTGYY146 pKa = 10.6KK147 pKa = 10.53GFDD150 pKa = 2.61WGAWHH155 pKa = 7.52DD156 pKa = 4.11GDD158 pKa = 5.13DD159 pKa = 4.37AAIQKK164 pKa = 9.68EE165 pKa = 3.94ADD167 pKa = 3.51GNHH170 pKa = 5.84YY171 pKa = 10.76LSGGVFWTPIQAADD185 pKa = 3.31GGTFQIEE192 pKa = 4.34QFSVANGTSDD202 pKa = 3.68YY203 pKa = 11.61DD204 pKa = 3.56NLLTIEE210 pKa = 4.38GRR212 pKa = 11.84LNGDD216 pKa = 3.39TVFLVTVNVTADD228 pKa = 4.0SIHH231 pKa = 7.07DD232 pKa = 3.82PQVIDD237 pKa = 4.05LSAYY241 pKa = 9.53GAIDD245 pKa = 3.86YY246 pKa = 10.27LVLDD250 pKa = 4.28TEE252 pKa = 4.46PVITEE257 pKa = 3.99TSGPDD262 pKa = 3.43YY263 pKa = 11.38YY264 pKa = 11.19GAQYY268 pKa = 11.49DD269 pKa = 3.71NFHH272 pKa = 6.44FVVV275 pKa = 3.71

Molecular weight: 29.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2ADV5|F2ADV5_RHIET GHMP kinase OS=Rhizobium etli CNPAF512 OX=993047 GN=RHECNPAF_440019 PE=4 SV=1
MM1 pKa = 6.99NKK3 pKa = 9.43SVRR6 pKa = 11.84RR7 pKa = 11.84HH8 pKa = 5.16RR9 pKa = 11.84VPPGGTRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84NRR20 pKa = 11.84LHH22 pKa = 6.59SSPPIRR28 pKa = 11.84GNFRR32 pKa = 11.84RR33 pKa = 4.0

Molecular weight: 3.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6543

0

6543

1785383

20

2847

272.9

29.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.866 ± 0.042

0.991 ± 0.013

5.707 ± 0.026

5.684 ± 0.029

3.939 ± 0.022

8.335 ± 0.031

2.209 ± 0.018

5.632 ± 0.021

3.565 ± 0.023

9.811 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.51 ± 0.014

2.78 ± 0.017

4.961 ± 0.023

3.22 ± 0.019

7.348 ± 0.037

5.945 ± 0.028

5.0 ± 0.023

7.063 ± 0.023

1.247 ± 0.012

2.187 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski