Aquareovirus C (isolate Golden shiner/USA/GSRV/1977) (AQRV-C)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Aquareovirus; Aquareovirus C

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q8JU56|VNS5_AQRVC Non-structural protein 5 OS=Aquareovirus C (isolate Golden shiner/USA/GSRV/1977) OX=185783 GN=S7 PE=3 SV=1
MM1 pKa = 7.18NADD4 pKa = 3.66RR5 pKa = 11.84TLSDD9 pKa = 3.74EE10 pKa = 4.08QYY12 pKa = 10.63DD13 pKa = 3.69WIVAYY18 pKa = 9.59EE19 pKa = 3.86RR20 pKa = 11.84HH21 pKa = 5.36ITPHH25 pKa = 6.1AVLSVRR31 pKa = 11.84TPAYY35 pKa = 10.49GRR37 pKa = 11.84MPPLQIPTFTGPTPTYY53 pKa = 9.83FVYY56 pKa = 10.57DD57 pKa = 3.78GNVEE61 pKa = 4.19EE62 pKa = 5.91VSGQLIKK69 pKa = 11.05DD70 pKa = 3.87PSDD73 pKa = 3.3DD74 pKa = 4.07TLPHH78 pKa = 6.78HH79 pKa = 6.77YY80 pKa = 10.2AATWTCSLFTSRR92 pKa = 11.84PPSDD96 pKa = 3.66PAWPRR101 pKa = 11.84DD102 pKa = 3.23SVMQYY107 pKa = 11.04DD108 pKa = 4.78SNWSPYY114 pKa = 8.9HH115 pKa = 5.87RR116 pKa = 11.84VPMRR120 pKa = 11.84YY121 pKa = 8.63FPSFMTLDD129 pKa = 3.76SDD131 pKa = 5.21LLGPNDD137 pKa = 4.11GFWPLLHH144 pKa = 6.75RR145 pKa = 11.84CAGFRR150 pKa = 11.84LRR152 pKa = 11.84SPWRR156 pKa = 11.84FPLDD160 pKa = 3.19VLAAGIGTTVSTIRR174 pKa = 11.84SLEE177 pKa = 3.84KK178 pKa = 10.51AGALNTVEE186 pKa = 4.17GLLRR190 pKa = 11.84ALGWKK195 pKa = 8.72YY196 pKa = 10.52HH197 pKa = 6.15HH198 pKa = 7.15LSGLRR203 pKa = 11.84PQHH206 pKa = 6.19VEE208 pKa = 3.4YY209 pKa = 10.97CMSVWSLSFTRR220 pKa = 11.84ADD222 pKa = 4.31LDD224 pKa = 4.26VIDD227 pKa = 4.42PASWFDD233 pKa = 3.41GLLRR237 pKa = 11.84SPIYY241 pKa = 9.98PDD243 pKa = 3.37EE244 pKa = 4.98APIGLNCEE252 pKa = 4.39QITTHH257 pKa = 6.89PFVLLNWIRR266 pKa = 11.84VQLMDD271 pKa = 5.54DD272 pKa = 3.89GCC274 pKa = 5.48

Molecular weight: 31.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q8JU62|VP1_AQRVC Outer capsid protein VP1 OS=Aquareovirus C (isolate Golden shiner/USA/GSRV/1977) OX=185783 GN=S1 PE=3 SV=1
MM1 pKa = 7.73EE2 pKa = 5.13EE3 pKa = 4.82LFNALPQPLQQLSLALAGEE22 pKa = 4.49IPLTDD27 pKa = 4.77HH28 pKa = 7.01IFEE31 pKa = 4.34QAASTWHH38 pKa = 4.84VQPRR42 pKa = 11.84SLTYY46 pKa = 10.7KK47 pKa = 10.62LLDD50 pKa = 4.74HH51 pKa = 7.1IPFSTPVVVPPSIYY65 pKa = 10.23HH66 pKa = 5.55SLDD69 pKa = 2.72WSKK72 pKa = 11.24CFAVNQDD79 pKa = 2.89RR80 pKa = 11.84VEE82 pKa = 4.1RR83 pKa = 11.84VPTIDD88 pKa = 5.54DD89 pKa = 4.18PDD91 pKa = 3.8DD92 pKa = 4.39VYY94 pKa = 11.86VPNSDD99 pKa = 3.39IGPLLTSLHH108 pKa = 6.64TIPDD112 pKa = 3.66YY113 pKa = 11.61GFLHH117 pKa = 6.7PAIEE121 pKa = 4.33NDD123 pKa = 3.08ATTLRR128 pKa = 11.84AEE130 pKa = 4.57RR131 pKa = 11.84ARR133 pKa = 11.84CASTFYY139 pKa = 10.83KK140 pKa = 10.18IASSQARR147 pKa = 11.84QVKK150 pKa = 10.05LDD152 pKa = 4.51PIRR155 pKa = 11.84MLGFLLLVQARR166 pKa = 11.84PRR168 pKa = 11.84VPSGLVTDD176 pKa = 3.81QPTRR180 pKa = 11.84RR181 pKa = 11.84DD182 pKa = 3.78PTQSPALHH190 pKa = 6.92AIWQVMQYY198 pKa = 10.07YY199 pKa = 9.57KK200 pKa = 10.9VAGVYY205 pKa = 7.47YY206 pKa = 10.58APALVVPSGAIWWIPPPGKK225 pKa = 10.23RR226 pKa = 11.84NVVSVQYY233 pKa = 11.38LLTDD237 pKa = 5.15LINLAILAHH246 pKa = 5.6MTDD249 pKa = 3.63MSPTLEE255 pKa = 4.07LTGVLMYY262 pKa = 10.73LRR264 pKa = 11.84AASSHH269 pKa = 4.04SHH271 pKa = 6.99AYY273 pKa = 8.97TLLQMKK279 pKa = 9.88SVFPALSLRR288 pKa = 11.84SMYY291 pKa = 10.3RR292 pKa = 11.84NKK294 pKa = 10.65GFGGKK299 pKa = 9.77APAIEE304 pKa = 4.1WTEE307 pKa = 3.62PRR309 pKa = 11.84SKK311 pKa = 11.27YY312 pKa = 9.63KK313 pKa = 10.5FRR315 pKa = 11.84WTGVTQLHH323 pKa = 7.07DD324 pKa = 3.89GLRR327 pKa = 11.84PRR329 pKa = 11.84SPSMDD334 pKa = 3.25VPTLEE339 pKa = 4.48VLTKK343 pKa = 10.81YY344 pKa = 10.37EE345 pKa = 4.25LVDD348 pKa = 3.16IGHH351 pKa = 6.92IIIRR355 pKa = 11.84EE356 pKa = 4.1RR357 pKa = 11.84NAHH360 pKa = 6.25PRR362 pKa = 11.84HH363 pKa = 5.53NHH365 pKa = 5.43DD366 pKa = 2.85SVRR369 pKa = 11.84FVRR372 pKa = 11.84DD373 pKa = 3.1VMALTSGMYY382 pKa = 10.46LVRR385 pKa = 11.84QPTMSVLRR393 pKa = 11.84EE394 pKa = 3.88YY395 pKa = 11.18SQVPDD400 pKa = 3.85IKK402 pKa = 11.29DD403 pKa = 4.42PIPPSAWTGPIGNVRR418 pKa = 11.84YY419 pKa = 9.5LLPSVQGPARR429 pKa = 11.84HH430 pKa = 6.76LYY432 pKa = 8.49DD433 pKa = 3.09TWRR436 pKa = 11.84AAARR440 pKa = 11.84QIAQDD445 pKa = 4.16PQWHH449 pKa = 7.28DD450 pKa = 3.82PLNQAIMRR458 pKa = 11.84AQYY461 pKa = 8.14VTARR465 pKa = 11.84GGSSASLKK473 pKa = 10.36FALKK477 pKa = 8.98VTGIVLPEE485 pKa = 3.99YY486 pKa = 10.67DD487 pKa = 3.62DD488 pKa = 5.01SKK490 pKa = 11.23VKK492 pKa = 10.5KK493 pKa = 10.12SSKK496 pKa = 9.79IYY498 pKa = 10.01QAAQIARR505 pKa = 11.84IAFMLLIAAIHH516 pKa = 6.5AEE518 pKa = 3.95VTMGIRR524 pKa = 11.84NQVQRR529 pKa = 11.84RR530 pKa = 11.84ARR532 pKa = 11.84SIMPLNVIQQAISAPHH548 pKa = 5.92TLVANYY554 pKa = 9.64INKK557 pKa = 9.18HH558 pKa = 5.22MNLSTTSGSVVTDD571 pKa = 3.15KK572 pKa = 11.2VIPLILYY579 pKa = 10.2ASTPPNTVVNVDD591 pKa = 3.74IKK593 pKa = 11.38ACDD596 pKa = 3.18ASITYY601 pKa = 10.53NYY603 pKa = 9.11FLSVICGAMHH613 pKa = 7.0EE614 pKa = 4.4GFEE617 pKa = 4.76VGNADD622 pKa = 3.65AAFMGVPSTIVSDD635 pKa = 3.64RR636 pKa = 11.84RR637 pKa = 11.84SSVAPYY643 pKa = 10.02SRR645 pKa = 11.84PISGLQTMVQHH656 pKa = 7.38LADD659 pKa = 4.81LYY661 pKa = 11.04AAGFRR666 pKa = 11.84YY667 pKa = 9.84SVSDD671 pKa = 3.64AFSSGNKK678 pKa = 9.41FSFPTSTFPSGSTATSTEE696 pKa = 3.82HH697 pKa = 5.93TANNSTMMEE706 pKa = 4.1YY707 pKa = 10.41FLNVHH712 pKa = 6.53APSHH716 pKa = 5.94VKK718 pKa = 10.06SASLKK723 pKa = 10.59RR724 pKa = 11.84ILTDD728 pKa = 2.95MTIQRR733 pKa = 11.84NYY735 pKa = 9.14VCQGDD740 pKa = 4.24DD741 pKa = 4.41GILLLPHH748 pKa = 6.64EE749 pKa = 4.97AASKK753 pKa = 10.53ISADD757 pKa = 4.57DD758 pKa = 3.56MNEE761 pKa = 3.78LLTCLRR767 pKa = 11.84DD768 pKa = 3.41YY769 pKa = 11.34GQLFGWNYY777 pKa = 10.8DD778 pKa = 3.42IDD780 pKa = 3.75WSDD783 pKa = 2.96TAEE786 pKa = 4.01YY787 pKa = 10.34LKK789 pKa = 10.83LYY791 pKa = 10.84ALMGCRR797 pKa = 11.84IPNTSRR803 pKa = 11.84HH804 pKa = 5.45PPVGKK809 pKa = 10.2EE810 pKa = 3.6YY811 pKa = 10.62AAPQTDD817 pKa = 4.78EE818 pKa = 4.24IWPSLIDD825 pKa = 3.51IVIGHH830 pKa = 7.03HH831 pKa = 6.42LNGVTDD837 pKa = 3.57VLNWRR842 pKa = 11.84EE843 pKa = 3.68WLRR846 pKa = 11.84FSWAFACYY854 pKa = 9.64SSRR857 pKa = 11.84GGYY860 pKa = 7.43TNPKK864 pKa = 8.18GQSFSAQYY872 pKa = 9.27PWWTFVYY879 pKa = 10.48LGIPPILLPGQTPFIHH895 pKa = 6.07SCYY898 pKa = 9.31MPPGDD903 pKa = 3.84QGMFSILNGWRR914 pKa = 11.84DD915 pKa = 3.52WLISHH920 pKa = 7.54ASTTLPPLRR929 pKa = 11.84HH930 pKa = 5.74NHH932 pKa = 5.65PVWGLSDD939 pKa = 3.85VPSLLSQFGVYY950 pKa = 10.06AGYY953 pKa = 9.45HH954 pKa = 5.61AAQHH958 pKa = 5.04YY959 pKa = 8.15RR960 pKa = 11.84RR961 pKa = 11.84PKK963 pKa = 9.11PAPEE967 pKa = 4.04TASSDD972 pKa = 3.81SINQITSDD980 pKa = 3.14LTEE983 pKa = 3.76YY984 pKa = 11.0LFYY987 pKa = 11.13DD988 pKa = 3.45SALKK992 pKa = 10.45ARR994 pKa = 11.84VMKK997 pKa = 10.53GRR999 pKa = 11.84YY1000 pKa = 6.51NWEE1003 pKa = 4.0RR1004 pKa = 11.84LSSSLSLNVGSRR1016 pKa = 11.84VPSLFDD1022 pKa = 4.08VPGKK1026 pKa = 9.32WVAAGRR1032 pKa = 11.84DD1033 pKa = 3.71AEE1035 pKa = 4.41KK1036 pKa = 10.33PPPSSVEE1043 pKa = 4.29DD1044 pKa = 3.38MFTSLNRR1051 pKa = 11.84CIRR1054 pKa = 11.84RR1055 pKa = 11.84PTHH1058 pKa = 5.52SFSRR1062 pKa = 11.84LLEE1065 pKa = 4.29LYY1067 pKa = 10.53LRR1069 pKa = 11.84VHH1071 pKa = 5.24VTLGEE1076 pKa = 4.49SIPLAIDD1083 pKa = 3.45PDD1085 pKa = 4.17VPQVAGADD1093 pKa = 3.91PANDD1097 pKa = 3.66DD1098 pKa = 3.07HH1099 pKa = 6.34WFKK1102 pKa = 10.26YY1103 pKa = 8.46TCLGDD1108 pKa = 3.45IPSATRR1114 pKa = 11.84NYY1116 pKa = 9.9FGEE1119 pKa = 4.17SLFVGRR1125 pKa = 11.84VVSGLDD1131 pKa = 3.37VEE1133 pKa = 5.74AVDD1136 pKa = 4.45ATLLRR1141 pKa = 11.84LKK1143 pKa = 10.52ILGAPPEE1150 pKa = 4.04AFIAVLNGIGMSDD1163 pKa = 3.74SEE1165 pKa = 4.21AHH1167 pKa = 5.85QIAGRR1172 pKa = 11.84ISLANAQLVQIARR1185 pKa = 11.84VVHH1188 pKa = 6.07LSIPSSWMTLNTGPYY1203 pKa = 8.0IHH1205 pKa = 6.74HH1206 pKa = 7.05HH1207 pKa = 6.73AYY1209 pKa = 10.32DD1210 pKa = 4.61FKK1212 pKa = 11.23PGITQPSAKK1221 pKa = 10.26SRR1223 pKa = 11.84DD1224 pKa = 3.32KK1225 pKa = 10.91SIWMSPILKK1234 pKa = 9.83LLCTSYY1240 pKa = 11.76AMTVAGPVRR1249 pKa = 11.84TSIVTEE1255 pKa = 3.83IDD1257 pKa = 2.96GSAAALSGNLRR1268 pKa = 11.84VWMRR1272 pKa = 11.84DD1273 pKa = 3.05VV1274 pKa = 3.21

Molecular weight: 141.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

7609

146

1299

634.1

69.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.737 ± 0.646

1.222 ± 0.225

5.664 ± 0.261

2.799 ± 0.208

3.364 ± 0.276

5.047 ± 0.33

2.589 ± 0.394

4.981 ± 0.215

1.919 ± 0.323

10.277 ± 0.358

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.431 ± 0.264

3.746 ± 0.419

7.964 ± 0.238

3.614 ± 0.244

5.599 ± 0.292

8.043 ± 0.416

8.135 ± 0.558

7.425 ± 0.313

1.406 ± 0.24

3.036 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski