Hyaloperonospora arabidopsidis (strain Emoy2) (Downy mildew agent) (Peronospora arabidopsidis)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae; Hyaloperonospora; Hyaloperonospora arabidopsidis

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13837 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4C067|M4C067_HYAAE Uncharacterized protein OS=Hyaloperonospora arabidopsidis (strain Emoy2) OX=559515 PE=4 SV=1
MM1 pKa = 7.48TISKK5 pKa = 7.62THH7 pKa = 5.67HH8 pKa = 6.09TLLLGAAALALDD20 pKa = 3.91AVNAHH25 pKa = 7.17GYY27 pKa = 9.34CSRR30 pKa = 11.84PAVTFPNGGDD40 pKa = 3.27TTQFVATIEE49 pKa = 4.37SSASGLPGVFTGSPADD65 pKa = 3.43NAAAFWTAFGSSKK78 pKa = 10.24FSSIKK83 pKa = 10.68DD84 pKa = 3.41LATSLGQIVAKK95 pKa = 10.34GATLEE100 pKa = 4.98CGLTDD105 pKa = 4.85PNGTPQPIPDD115 pKa = 3.78EE116 pKa = 4.89LEE118 pKa = 3.92WSHH121 pKa = 7.24SGTEE125 pKa = 4.27GFTASHH131 pKa = 7.03EE132 pKa = 4.82GPCEE136 pKa = 3.74AWCDD140 pKa = 3.75DD141 pKa = 3.62TQVFQDD147 pKa = 4.25EE148 pKa = 4.15NCAAHH153 pKa = 6.37FTTAPAKK160 pKa = 9.85MPYY163 pKa = 10.38DD164 pKa = 3.47KK165 pKa = 10.72AKK167 pKa = 10.72CSGASRR173 pKa = 11.84FTFYY177 pKa = 10.5WLALHH182 pKa = 6.73SSTWQVYY189 pKa = 6.79VNCAALEE196 pKa = 4.45GGSGDD201 pKa = 3.82MYY203 pKa = 10.37STSQTEE209 pKa = 4.33VNASEE214 pKa = 4.44STEE217 pKa = 3.92QSSPPTSSSDD227 pKa = 3.16TPTAVVAPDD236 pKa = 3.14QSYY239 pKa = 11.38SFTGTTAAPSTTDD252 pKa = 3.25VPVNTSKK259 pKa = 9.69STGEE263 pKa = 4.14STTPAPSNATAPADD277 pKa = 3.6VVPTSTPSPTGEE289 pKa = 3.97PSFTFADD296 pKa = 4.0TNAMPNTTEE305 pKa = 3.96AVAPNTTKK313 pKa = 10.54IIGSNTSEE321 pKa = 4.18TVAPSTPAPTDD332 pKa = 3.53PSNTPSAPIATPTTEE347 pKa = 4.34VISPTGDD354 pKa = 2.44ITTSSNSDD362 pKa = 3.39VNSVGVVGIKK372 pKa = 10.09DD373 pKa = 3.58DD374 pKa = 4.58CGSLDD379 pKa = 3.52MAGEE383 pKa = 4.26GDD385 pKa = 3.85NVVKK389 pKa = 10.91ANTDD393 pKa = 2.84ISSSSNSGVGPDD405 pKa = 3.44VNTVGVVEE413 pKa = 4.66TTATPNTTEE422 pKa = 4.95AIAPNTTGAIAPNTSKK438 pKa = 10.73IIGSNTSEE446 pKa = 4.18TVAPSTPAPTDD457 pKa = 3.5PSNTPTATTTTPTTEE472 pKa = 4.34VISPTGGITTSSNSGVGSDD491 pKa = 3.7VNAVSVVGIEE501 pKa = 4.34DD502 pKa = 3.79DD503 pKa = 4.55CGSLDD508 pKa = 3.71MAGEE512 pKa = 4.18GDD514 pKa = 3.6NRR516 pKa = 11.84VEE518 pKa = 4.21TSTVPVPDD526 pKa = 4.17EE527 pKa = 4.59DD528 pKa = 4.58CGSFDD533 pKa = 3.46IAGSEE538 pKa = 4.09DD539 pKa = 3.71MEE541 pKa = 5.11DD542 pKa = 4.27CGSLDD547 pKa = 3.19IAGGSLDD554 pKa = 3.72GVGSSGIDD562 pKa = 3.23NRR564 pKa = 11.84ITSDD568 pKa = 3.39SVGSFAGEE576 pKa = 3.79VNPAISEE583 pKa = 4.19SSLNFDD589 pKa = 4.53DD590 pKa = 4.54VDD592 pKa = 3.5RR593 pKa = 11.84GYY595 pKa = 9.53TGGKK599 pKa = 8.18VQAPYY604 pKa = 10.64QYY606 pKa = 11.71

Molecular weight: 61.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4B3H1|M4B3H1_HYAAE Uncharacterized protein OS=Hyaloperonospora arabidopsidis (strain Emoy2) OX=559515 PE=4 SV=1
MM1 pKa = 7.16VRR3 pKa = 11.84RR4 pKa = 11.84LRR6 pKa = 11.84SGAVPARR13 pKa = 11.84LGRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84LIARR22 pKa = 11.84VWTKK26 pKa = 10.59RR27 pKa = 11.84DD28 pKa = 3.1QTTRR32 pKa = 11.84RR33 pKa = 11.84HH34 pKa = 4.41QRR36 pKa = 11.84RR37 pKa = 11.84PRR39 pKa = 11.84AIAAAMALTRR49 pKa = 11.84HH50 pKa = 5.98KK51 pKa = 10.12LAKK54 pKa = 9.44GVRR57 pKa = 11.84GVRR60 pKa = 11.84ILLPAVEE67 pKa = 4.48TPRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84LLRR75 pKa = 11.84RR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84IPRR81 pKa = 11.84HH82 pKa = 3.73HH83 pKa = 6.02HH84 pKa = 3.57QRR86 pKa = 11.84RR87 pKa = 11.84RR88 pKa = 11.84RR89 pKa = 11.84RR90 pKa = 11.84RR91 pKa = 11.84RR92 pKa = 11.84RR93 pKa = 11.84VTLRR97 pKa = 11.84RR98 pKa = 11.84RR99 pKa = 11.84LWPLRR104 pKa = 11.84SRR106 pKa = 11.84RR107 pKa = 11.84LRR109 pKa = 11.84ARR111 pKa = 11.84TVHH114 pKa = 6.82RR115 pKa = 11.84PLQRR119 pKa = 11.84VRR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 11.84GRR125 pKa = 11.84LQTCRR130 pKa = 11.84RR131 pKa = 11.84LL132 pKa = 3.43

Molecular weight: 16.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13837

0

13837

4494721

49

4812

324.8

36.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.569 ± 0.021

1.894 ± 0.011

5.672 ± 0.015

6.331 ± 0.023

3.618 ± 0.015

6.061 ± 0.018

2.561 ± 0.011

4.081 ± 0.015

5.083 ± 0.022

9.452 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.563 ± 0.009

3.331 ± 0.011

4.453 ± 0.017

4.143 ± 0.016

6.697 ± 0.023

8.416 ± 0.025

5.904 ± 0.015

7.406 ± 0.018

1.211 ± 0.007

2.529 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski