Bacillus sp. FJAT-18017

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4632 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M3RFZ7|A0A0M3RFZ7_9BACI Uncharacterized protein OS=Bacillus sp. FJAT-18017 OX=1705566 GN=AM500_07570 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.43ANPDD6 pKa = 4.13DD7 pKa = 4.6IYY9 pKa = 11.27DD10 pKa = 3.8IFDD13 pKa = 3.78NSDD16 pKa = 4.1DD17 pKa = 3.87IQEE20 pKa = 4.54LEE22 pKa = 4.53LDD24 pKa = 4.66IDD26 pKa = 4.35TSWHH30 pKa = 6.22GIYY33 pKa = 10.52FLLTGDD39 pKa = 3.76TAKK42 pKa = 11.07DD43 pKa = 3.44QVPDD47 pKa = 4.14NIGKK51 pKa = 9.77AVLGGTEE58 pKa = 3.95IGDD61 pKa = 3.9DD62 pKa = 3.76FSDD65 pKa = 3.89YY66 pKa = 11.26GPARR70 pKa = 11.84YY71 pKa = 9.17FEE73 pKa = 4.86PNEE76 pKa = 3.8VAQLYY81 pKa = 10.56SEE83 pKa = 5.27LKK85 pKa = 10.32QIPASQLAEE94 pKa = 4.15RR95 pKa = 11.84YY96 pKa = 9.85DD97 pKa = 3.77IQTLNANNIYY107 pKa = 10.51PMGKK111 pKa = 8.64RR112 pKa = 11.84WSPDD116 pKa = 3.03DD117 pKa = 3.9KK118 pKa = 11.31EE119 pKa = 4.65FLIEE123 pKa = 4.36YY124 pKa = 10.68YY125 pKa = 10.74DD126 pKa = 4.03FLVNYY131 pKa = 9.11YY132 pKa = 8.44KK133 pKa = 10.31TAAEE137 pKa = 4.29NNQAMLLVIGG147 pKa = 4.55

Molecular weight: 16.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M3RDL8|A0A0M3RDL8_9BACI NADPH-dependent 7-cyano-7-deazaguanine reductase OS=Bacillus sp. FJAT-18017 OX=1705566 GN=queF PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.87VHH16 pKa = 5.77GFRR19 pKa = 11.84KK20 pKa = 9.88RR21 pKa = 11.84MSSATGRR28 pKa = 11.84KK29 pKa = 8.4VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.36GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4632

0

4632

1341410

27

3050

289.6

32.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.457 ± 0.038

0.684 ± 0.01

4.996 ± 0.026

7.458 ± 0.043

4.692 ± 0.03

7.369 ± 0.029

1.904 ± 0.018

7.648 ± 0.034

6.809 ± 0.03

9.835 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.691 ± 0.017

4.297 ± 0.028

3.783 ± 0.019

3.347 ± 0.021

4.163 ± 0.028

6.064 ± 0.028

5.321 ± 0.025

6.909 ± 0.025

1.064 ± 0.015

3.508 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski