Amino acid dipepetide frequency for Bacillus sp. FJAT-18017

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.882AlaAla: 6.882 ± 0.096
0.565AlaCys: 0.565 ± 0.023
3.621AlaAsp: 3.621 ± 0.064
5.26AlaGlu: 5.26 ± 0.071
3.472AlaPhe: 3.472 ± 0.059
6.359AlaGly: 6.359 ± 0.07
1.25AlaHis: 1.25 ± 0.03
6.06AlaIle: 6.06 ± 0.082
4.671AlaLys: 4.671 ± 0.064
7.477AlaLeu: 7.477 ± 0.098
2.127AlaMet: 2.127 ± 0.041
2.867AlaAsn: 2.867 ± 0.054
2.233AlaPro: 2.233 ± 0.044
2.111AlaGln: 2.111 ± 0.038
3.103AlaArg: 3.103 ± 0.046
4.416AlaSer: 4.416 ± 0.063
3.364AlaThr: 3.364 ± 0.052
5.676AlaVal: 5.676 ± 0.066
0.693AlaTrp: 0.693 ± 0.026
2.363AlaTyr: 2.363 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.409CysAla: 0.409 ± 0.018
0.073CysCys: 0.073 ± 0.008
0.339CysAsp: 0.339 ± 0.016
0.46CysGlu: 0.46 ± 0.019
0.313CysPhe: 0.313 ± 0.016
0.658CysGly: 0.658 ± 0.027
0.227CysHis: 0.227 ± 0.019
0.496CysIle: 0.496 ± 0.021
0.376CysLys: 0.376 ± 0.015
0.696CysLeu: 0.696 ± 0.024
0.155CysMet: 0.155 ± 0.011
0.253CysAsn: 0.253 ± 0.014
0.359CysPro: 0.359 ± 0.019
0.201CysGln: 0.201 ± 0.011
0.28CysArg: 0.28 ± 0.015
0.493CysSer: 0.493 ± 0.02
0.377CysThr: 0.377 ± 0.019
0.372CysVal: 0.372 ± 0.018
0.06CysTrp: 0.06 ± 0.006
0.244CysTyr: 0.244 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.313AspAla: 3.313 ± 0.05
0.365AspCys: 0.365 ± 0.018
2.216AspAsp: 2.216 ± 0.046
4.108AspGlu: 4.108 ± 0.063
2.549AspPhe: 2.549 ± 0.047
3.692AspGly: 3.692 ± 0.051
0.983AspHis: 0.983 ± 0.027
4.054AspIle: 4.054 ± 0.057
3.194AspLys: 3.194 ± 0.052
4.819AspLeu: 4.819 ± 0.07
1.269AspMet: 1.269 ± 0.03
1.823AspAsn: 1.823 ± 0.044
2.081AspPro: 2.081 ± 0.043
1.653AspGln: 1.653 ± 0.039
2.303AspArg: 2.303 ± 0.047
2.862AspSer: 2.862 ± 0.047
2.404AspThr: 2.404 ± 0.042
3.495AspVal: 3.495 ± 0.051
0.639AspTrp: 0.639 ± 0.022
2.137AspTyr: 2.137 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
5.592GluAla: 5.592 ± 0.078
0.382GluCys: 0.382 ± 0.017
3.628GluAsp: 3.628 ± 0.063
6.618GluGlu: 6.618 ± 0.092
2.945GluPhe: 2.945 ± 0.051
4.711GluGly: 4.711 ± 0.062
1.351GluHis: 1.351 ± 0.034
5.523GluIle: 5.523 ± 0.078
6.41GluLys: 6.41 ± 0.088
7.26GluLeu: 7.26 ± 0.084
2.251GluMet: 2.251 ± 0.046
3.528GluAsn: 3.528 ± 0.053
2.123GluPro: 2.123 ± 0.038
2.996GluGln: 2.996 ± 0.053
3.504GluArg: 3.504 ± 0.057
3.514GluSer: 3.514 ± 0.052
4.088GluThr: 4.088 ± 0.049
4.952GluVal: 4.952 ± 0.067
0.859GluTrp: 0.859 ± 0.028
2.348GluTyr: 2.348 ± 0.038
0.0GluXaa: 0.0 ± 0.0
Phe
3.33PheAla: 3.33 ± 0.058
0.362PheCys: 0.362 ± 0.019
2.356PheAsp: 2.356 ± 0.038
3.016PheGlu: 3.016 ± 0.052
2.441PhePhe: 2.441 ± 0.051
3.573PheGly: 3.573 ± 0.058
0.906PheHis: 0.906 ± 0.029
3.792PheIle: 3.792 ± 0.063
2.585PheLys: 2.585 ± 0.043
4.925PheLeu: 4.925 ± 0.072
1.184PheMet: 1.184 ± 0.028
1.993PheAsn: 1.993 ± 0.034
1.778PhePro: 1.778 ± 0.038
1.483PheGln: 1.483 ± 0.034
1.701PheArg: 1.701 ± 0.039
3.404PheSer: 3.404 ± 0.052
2.673PheThr: 2.673 ± 0.051
3.068PheVal: 3.068 ± 0.056
0.526PheTrp: 0.526 ± 0.023
1.823PheTyr: 1.823 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
5.211GlyAla: 5.211 ± 0.072
0.625GlyCys: 0.625 ± 0.025
3.23GlyAsp: 3.23 ± 0.055
4.741GlyGlu: 4.741 ± 0.064
3.656GlyPhe: 3.656 ± 0.056
5.307GlyGly: 5.307 ± 0.083
1.419GlyHis: 1.419 ± 0.033
6.328GlyIle: 6.328 ± 0.07
5.547GlyLys: 5.547 ± 0.06
7.213GlyLeu: 7.213 ± 0.075
2.209GlyMet: 2.209 ± 0.041
3.063GlyAsn: 3.063 ± 0.058
1.931GlyPro: 1.931 ± 0.041
2.326GlyGln: 2.326 ± 0.046
3.026GlyArg: 3.026 ± 0.05
4.325GlySer: 4.325 ± 0.059
4.564GlyThr: 4.564 ± 0.067
5.136GlyVal: 5.136 ± 0.066
0.878GlyTrp: 0.878 ± 0.03
2.96GlyTyr: 2.96 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
1.234HisAla: 1.234 ± 0.03
0.186HisCys: 0.186 ± 0.013
0.945HisAsp: 0.945 ± 0.025
1.265HisGlu: 1.265 ± 0.031
0.989HisPhe: 0.989 ± 0.027
1.384HisGly: 1.384 ± 0.034
0.52HisHis: 0.52 ± 0.023
1.398HisIle: 1.398 ± 0.035
0.986HisLys: 0.986 ± 0.028
1.877HisLeu: 1.877 ± 0.039
0.468HisMet: 0.468 ± 0.017
0.723HisAsn: 0.723 ± 0.019
1.087HisPro: 1.087 ± 0.034
0.693HisGln: 0.693 ± 0.025
0.836HisArg: 0.836 ± 0.023
1.182HisSer: 1.182 ± 0.033
0.983HisThr: 0.983 ± 0.029
1.226HisVal: 1.226 ± 0.027
0.215HisTrp: 0.215 ± 0.014
0.842HisTyr: 0.842 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
6.082IleAla: 6.082 ± 0.076
0.598IleCys: 0.598 ± 0.02
4.177IleAsp: 4.177 ± 0.066
5.447IleGlu: 5.447 ± 0.071
3.241IlePhe: 3.241 ± 0.066
6.06IleGly: 6.06 ± 0.084
1.501IleHis: 1.501 ± 0.037
5.686IleIle: 5.686 ± 0.077
4.504IleLys: 4.504 ± 0.063
7.427IleLeu: 7.427 ± 0.113
1.792IleMet: 1.792 ± 0.042
3.225IleAsn: 3.225 ± 0.058
3.45IlePro: 3.45 ± 0.05
2.533IleGln: 2.533 ± 0.045
3.247IleArg: 3.247 ± 0.055
5.035IleSer: 5.035 ± 0.065
4.079IleThr: 4.079 ± 0.059
5.401IleVal: 5.401 ± 0.078
0.679IleTrp: 0.679 ± 0.023
2.322IleTyr: 2.322 ± 0.044
0.0IleXaa: 0.0 ± 0.0
Lys
5.091LysAla: 5.091 ± 0.068
0.328LysCys: 0.328 ± 0.017
3.787LysAsp: 3.787 ± 0.053
6.469LysGlu: 6.469 ± 0.081
2.062LysPhe: 2.062 ± 0.042
4.783LysGly: 4.783 ± 0.064
1.15LysHis: 1.15 ± 0.03
4.623LysIle: 4.623 ± 0.054
5.599LysLys: 5.599 ± 0.083
5.946LysLeu: 5.946 ± 0.073
2.055LysMet: 2.055 ± 0.04
3.214LysAsn: 3.214 ± 0.051
2.442LysPro: 2.442 ± 0.046
2.699LysGln: 2.699 ± 0.05
3.229LysArg: 3.229 ± 0.051
3.399LysSer: 3.399 ± 0.053
3.608LysThr: 3.608 ± 0.055
4.716LysVal: 4.716 ± 0.065
0.819LysTrp: 0.819 ± 0.027
2.069LysTyr: 2.069 ± 0.04
0.0LysXaa: 0.0 ± 0.0
Leu
7.979LeuAla: 7.979 ± 0.087
0.604LeuCys: 0.604 ± 0.021
4.87LeuAsp: 4.87 ± 0.06
6.98LeuGlu: 6.98 ± 0.084
4.952LeuPhe: 4.952 ± 0.079
6.946LeuGly: 6.946 ± 0.087
1.752LeuHis: 1.752 ± 0.038
7.216LeuIle: 7.216 ± 0.098
6.732LeuLys: 6.732 ± 0.08
10.153LeuLeu: 10.153 ± 0.132
2.432LeuMet: 2.432 ± 0.046
4.208LeuAsn: 4.208 ± 0.058
4.017LeuPro: 4.017 ± 0.056
3.159LeuGln: 3.159 ± 0.054
3.644LeuArg: 3.644 ± 0.054
6.701LeuSer: 6.701 ± 0.075
5.494LeuThr: 5.494 ± 0.066
6.457LeuVal: 6.457 ± 0.074
0.86LeuTrp: 0.86 ± 0.027
3.191LeuTyr: 3.191 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
2.275MetAla: 2.275 ± 0.048
0.136MetCys: 0.136 ± 0.011
1.519MetAsp: 1.519 ± 0.033
2.051MetGlu: 2.051 ± 0.039
1.097MetPhe: 1.097 ± 0.031
1.85MetGly: 1.85 ± 0.037
0.439MetHis: 0.439 ± 0.02
1.938MetIle: 1.938 ± 0.041
2.29MetLys: 2.29 ± 0.04
2.502MetLeu: 2.502 ± 0.045
0.779MetMet: 0.779 ± 0.023
1.463MetAsn: 1.463 ± 0.032
1.085MetPro: 1.085 ± 0.027
0.784MetGln: 0.784 ± 0.023
1.036MetArg: 1.036 ± 0.028
1.536MetSer: 1.536 ± 0.031
1.416MetThr: 1.416 ± 0.033
1.802MetVal: 1.802 ± 0.041
0.18MetTrp: 0.18 ± 0.011
0.737MetTyr: 0.737 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
2.677AsnAla: 2.677 ± 0.047
0.279AsnCys: 0.279 ± 0.017
2.036AsnAsp: 2.036 ± 0.046
3.389AsnGlu: 3.389 ± 0.052
1.778AsnPhe: 1.778 ± 0.035
3.496AsnGly: 3.496 ± 0.064
0.909AsnHis: 0.909 ± 0.028
3.068AsnIle: 3.068 ± 0.05
2.791AsnLys: 2.791 ± 0.05
3.893AsnLeu: 3.893 ± 0.053
1.178AsnMet: 1.178 ± 0.032
1.847AsnAsn: 1.847 ± 0.041
2.376AsnPro: 2.376 ± 0.043
1.703AsnGln: 1.703 ± 0.038
2.01AsnArg: 2.01 ± 0.044
2.471AsnSer: 2.471 ± 0.042
2.108AsnThr: 2.108 ± 0.047
2.837AsnVal: 2.837 ± 0.043
0.537AsnTrp: 0.537 ± 0.022
1.594AsnTyr: 1.594 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
2.839ProAla: 2.839 ± 0.05
0.217ProCys: 0.217 ± 0.012
2.188ProAsp: 2.188 ± 0.04
3.344ProGlu: 3.344 ± 0.058
2.066ProPhe: 2.066 ± 0.043
2.86ProGly: 2.86 ± 0.051
0.769ProHis: 0.769 ± 0.021
2.753ProIle: 2.753 ± 0.044
2.209ProLys: 2.209 ± 0.044
3.606ProLeu: 3.606 ± 0.049
0.823ProMet: 0.823 ± 0.024
1.643ProAsn: 1.643 ± 0.035
1.12ProPro: 1.12 ± 0.031
1.123ProGln: 1.123 ± 0.039
1.202ProArg: 1.202 ± 0.03
2.342ProSer: 2.342 ± 0.045
1.823ProThr: 1.823 ± 0.041
3.099ProVal: 3.099 ± 0.05
0.386ProTrp: 0.386 ± 0.015
1.415ProTyr: 1.415 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
2.637GlnAla: 2.637 ± 0.048
0.197GlnCys: 0.197 ± 0.014
1.566GlnAsp: 1.566 ± 0.039
2.611GlnGlu: 2.611 ± 0.055
1.52GlnPhe: 1.52 ± 0.038
2.093GlnGly: 2.093 ± 0.047
0.663GlnHis: 0.663 ± 0.022
2.3GlnIle: 2.3 ± 0.044
2.456GlnLys: 2.456 ± 0.044
3.554GlnLeu: 3.554 ± 0.057
0.967GlnMet: 0.967 ± 0.027
1.434GlnAsn: 1.434 ± 0.029
1.21GlnPro: 1.21 ± 0.032
1.376GlnGln: 1.376 ± 0.036
1.365GlnArg: 1.365 ± 0.032
1.938GlnSer: 1.938 ± 0.039
1.81GlnThr: 1.81 ± 0.039
2.16GlnVal: 2.16 ± 0.043
0.375GlnTrp: 0.375 ± 0.018
1.239GlnTyr: 1.239 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
2.602ArgAla: 2.602 ± 0.045
0.256ArgCys: 0.256 ± 0.014
2.163ArgAsp: 2.163 ± 0.04
3.446ArgGlu: 3.446 ± 0.055
2.018ArgPhe: 2.018 ± 0.04
2.538ArgGly: 2.538 ± 0.045
0.779ArgHis: 0.779 ± 0.025
3.209ArgIle: 3.209 ± 0.047
3.226ArgLys: 3.226 ± 0.055
4.101ArgLeu: 4.101 ± 0.067
1.293ArgMet: 1.293 ± 0.035
2.092ArgAsn: 2.092 ± 0.042
1.422ArgPro: 1.422 ± 0.044
1.554ArgGln: 1.554 ± 0.038
1.958ArgArg: 1.958 ± 0.04
2.21ArgSer: 2.21 ± 0.038
2.13ArgThr: 2.13 ± 0.044
2.65ArgVal: 2.65 ± 0.046
0.446ArgTrp: 0.446 ± 0.018
1.536ArgTyr: 1.536 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
4.137SerAla: 4.137 ± 0.062
0.416SerCys: 0.416 ± 0.019
2.787SerAsp: 2.787 ± 0.048
4.046SerGlu: 4.046 ± 0.062
3.381SerPhe: 3.381 ± 0.054
4.905SerGly: 4.905 ± 0.071
1.177SerHis: 1.177 ± 0.026
4.908SerIle: 4.908 ± 0.073
3.792SerLys: 3.792 ± 0.055
6.242SerLeu: 6.242 ± 0.078
1.657SerMet: 1.657 ± 0.036
2.394SerAsn: 2.394 ± 0.05
2.166SerPro: 2.166 ± 0.043
1.974SerGln: 1.974 ± 0.04
2.504SerArg: 2.504 ± 0.041
4.035SerSer: 4.035 ± 0.055
3.043SerThr: 3.043 ± 0.049
4.175SerVal: 4.175 ± 0.061
0.696SerTrp: 0.696 ± 0.024
2.204SerTyr: 2.204 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
4.255ThrAla: 4.255 ± 0.064
0.32ThrCys: 0.32 ± 0.014
2.658ThrAsp: 2.658 ± 0.049
3.503ThrGlu: 3.503 ± 0.056
2.57ThrPhe: 2.57 ± 0.043
4.604ThrGly: 4.604 ± 0.066
1.011ThrHis: 1.011 ± 0.029
4.401ThrIle: 4.401 ± 0.065
3.154ThrLys: 3.154 ± 0.051
5.087ThrLeu: 5.087 ± 0.06
1.287ThrMet: 1.287 ± 0.03
2.225ThrAsn: 2.225 ± 0.043
2.257ThrPro: 2.257 ± 0.044
1.256ThrGln: 1.256 ± 0.027
1.9ThrArg: 1.9 ± 0.042
3.181ThrSer: 3.181 ± 0.053
2.793ThrThr: 2.793 ± 0.055
4.324ThrVal: 4.324 ± 0.071
0.554ThrTrp: 0.554 ± 0.021
1.871ThrTyr: 1.871 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
5.099ValAla: 5.099 ± 0.066
0.562ValCys: 0.562 ± 0.022
3.452ValAsp: 3.452 ± 0.052
4.664ValGlu: 4.664 ± 0.07
3.396ValPhe: 3.396 ± 0.052
4.511ValGly: 4.511 ± 0.069
1.267ValHis: 1.267 ± 0.028
5.513ValIle: 5.513 ± 0.083
4.618ValLys: 4.618 ± 0.067
6.962ValLeu: 6.962 ± 0.075
1.78ValMet: 1.78 ± 0.044
2.976ValAsn: 2.976 ± 0.047
2.942ValPro: 2.942 ± 0.044
2.126ValGln: 2.126 ± 0.033
2.698ValArg: 2.698 ± 0.05
4.646ValSer: 4.646 ± 0.07
4.061ValThr: 4.061 ± 0.06
4.725ValVal: 4.725 ± 0.07
0.692ValTrp: 0.692 ± 0.025
2.4ValTyr: 2.4 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.74TrpAla: 0.74 ± 0.025
0.082TrpCys: 0.082 ± 0.009
0.57TrpAsp: 0.57 ± 0.023
0.748TrpGlu: 0.748 ± 0.025
0.542TrpPhe: 0.542 ± 0.023
0.783TrpGly: 0.783 ± 0.025
0.199TrpHis: 0.199 ± 0.013
0.787TrpIle: 0.787 ± 0.026
0.763TrpLys: 0.763 ± 0.026
1.117TrpLeu: 1.117 ± 0.033
0.347TrpMet: 0.347 ± 0.015
0.533TrpAsn: 0.533 ± 0.018
0.282TrpPro: 0.282 ± 0.015
0.379TrpGln: 0.379 ± 0.015
0.42TrpArg: 0.42 ± 0.021
0.605TrpSer: 0.605 ± 0.022
0.517TrpThr: 0.517 ± 0.021
0.716TrpVal: 0.716 ± 0.026
0.149TrpTrp: 0.149 ± 0.009
0.361TrpTyr: 0.361 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.187TyrAla: 2.187 ± 0.037
0.289TyrCys: 0.289 ± 0.016
1.85TyrAsp: 1.85 ± 0.041
2.416TyrGlu: 2.416 ± 0.048
1.931TyrPhe: 1.931 ± 0.038
2.582TyrGly: 2.582 ± 0.041
0.767TyrHis: 0.767 ± 0.021
2.432TyrIle: 2.432 ± 0.046
2.184TyrLys: 2.184 ± 0.047
3.491TyrLeu: 3.491 ± 0.053
0.859TyrMet: 0.859 ± 0.025
1.472TyrAsn: 1.472 ± 0.034
1.466TyrPro: 1.466 ± 0.035
1.338TyrGln: 1.338 ± 0.035
1.665TyrArg: 1.665 ± 0.038
2.346TyrSer: 2.346 ± 0.044
1.876TyrThr: 1.876 ± 0.037
2.105TyrVal: 2.105 ± 0.042
0.399TyrTrp: 0.399 ± 0.018
1.426TyrTyr: 1.426 ± 0.042
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4632 proteins (1341410 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski