Fly associated circular virus 2
Average proteome isoelectric point is 8.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BP61|A0A346BP61_9VIRU Putative capsid protein OS=Fly associated circular virus 2 OX=2293282 PE=4 SV=1
MM1 pKa = 7.83 SSFRR5 pKa = 11.84 FQARR9 pKa = 11.84 YY10 pKa = 10.11 GLFTYY15 pKa = 7.58 SQCGRR20 pKa = 11.84 LRR22 pKa = 11.84 PQQVSEE28 pKa = 4.48 HH29 pKa = 5.12 FTSLGADD36 pKa = 3.66 HH37 pKa = 7.29 IIGRR41 pKa = 11.84 EE42 pKa = 3.79 THH44 pKa = 5.99 QDD46 pKa = 3.12 GGTHH50 pKa = 5.9 LHH52 pKa = 5.88 VFADD56 pKa = 4.56 FRR58 pKa = 11.84 RR59 pKa = 11.84 KK60 pKa = 9.94 YY61 pKa = 9.02 RR62 pKa = 11.84 TRR64 pKa = 11.84 DD65 pKa = 3.02 NRR67 pKa = 11.84 KK68 pKa = 8.04 FDD70 pKa = 3.6 VEE72 pKa = 4.33 GFHH75 pKa = 6.93 PNIVPSLRR83 pKa = 11.84 NPAGGWDD90 pKa = 3.84 YY91 pKa = 10.92 ATKK94 pKa = 10.83 DD95 pKa = 3.2 GDD97 pKa = 3.43 ICAGEE102 pKa = 4.27 LSRR105 pKa = 11.84 PDD107 pKa = 3.67 DD108 pKa = 3.77 EE109 pKa = 5.43 RR110 pKa = 11.84 GSRR113 pKa = 11.84 SRR115 pKa = 11.84 TADD118 pKa = 3.51 AGWDD122 pKa = 3.45 ALRR125 pKa = 11.84 AAEE128 pKa = 4.19 TRR130 pKa = 11.84 DD131 pKa = 3.33 DD132 pKa = 3.96 FFRR135 pKa = 11.84 IASEE139 pKa = 4.06 HH140 pKa = 6.19 LFSHH144 pKa = 6.53 LVKK147 pKa = 10.71 SFNSFSAYY155 pKa = 10.37 ADD157 pKa = 2.82 WKK159 pKa = 10.51 FRR161 pKa = 11.84 VDD163 pKa = 3.55 RR164 pKa = 11.84 TPYY167 pKa = 9.52 IHH169 pKa = 7.59 DD170 pKa = 4.32 PNVTFDD176 pKa = 4.76 LGQLHH181 pKa = 6.29 QLRR184 pKa = 11.84 EE185 pKa = 4.47 WIRR188 pKa = 11.84 GNLPPGNNRR197 pKa = 11.84 AFGEE201 pKa = 4.17 RR202 pKa = 11.84 GISLLLWGRR211 pKa = 11.84 SRR213 pKa = 11.84 TGKK216 pKa = 7.69 TWLARR221 pKa = 11.84 SFGSHH226 pKa = 6.83 CYY228 pKa = 10.22 FGGLFALDD236 pKa = 3.44 EE237 pKa = 4.33 YY238 pKa = 11.04 EE239 pKa = 4.67 YY240 pKa = 11.33 NDD242 pKa = 3.63 KK243 pKa = 10.87 VDD245 pKa = 3.48 YY246 pKa = 11.18 AVFDD250 pKa = 5.21 DD251 pKa = 3.75 IQGGFKK257 pKa = 10.57 YY258 pKa = 10.42 FPAYY262 pKa = 9.71 KK263 pKa = 9.97 SWLGQQAEE271 pKa = 5.02 FYY273 pKa = 9.88 CTDD276 pKa = 3.05 KK277 pKa = 10.79 FRR279 pKa = 11.84 KK280 pKa = 9.1 KK281 pKa = 10.52 KK282 pKa = 8.41 YY283 pKa = 9.24 IKK285 pKa = 9.54 WGRR288 pKa = 11.84 PCIWIMNEE296 pKa = 3.73 DD297 pKa = 4.63 PYY299 pKa = 10.22 TQEE302 pKa = 5.17 VDD304 pKa = 3.45 IDD306 pKa = 3.78 WLEE309 pKa = 4.06 KK310 pKa = 10.45 NCLIVHH316 pKa = 7.01 IDD318 pKa = 3.45 RR319 pKa = 11.84 PLYY322 pKa = 8.64 QTAA325 pKa = 3.5
Molecular weight: 37.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.991
IPC2_protein 7.263
IPC_protein 7.263
Toseland 7.088
ProMoST 7.761
Dawson 7.892
Bjellqvist 8.141
Wikipedia 7.819
Rodwell 7.892
Grimsley 7.19
Solomon 7.98
Lehninger 7.995
Nozaki 8.302
DTASelect 8.024
Thurlkill 8.068
EMBOSS 8.141
Sillero 8.346
Patrickios 4.279
IPC_peptide 7.98
IPC2_peptide 7.454
IPC2.peptide.svr19 7.354
Protein with the highest isoelectric point:
>tr|A0A346BP61|A0A346BP61_9VIRU Putative capsid protein OS=Fly associated circular virus 2 OX=2293282 PE=4 SV=1
MM1 pKa = 8.02 AYY3 pKa = 10.14 RR4 pKa = 11.84 FRR6 pKa = 11.84 RR7 pKa = 11.84 FSTRR11 pKa = 11.84 SRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 FTPRR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 SSFRR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 NYY29 pKa = 10.64 RR30 pKa = 11.84 MIRR33 pKa = 11.84 RR34 pKa = 11.84 PRR36 pKa = 11.84 RR37 pKa = 11.84 FSRR40 pKa = 11.84 PRR42 pKa = 11.84 TNVRR46 pKa = 11.84 RR47 pKa = 11.84 VRR49 pKa = 11.84 NLASRR54 pKa = 11.84 KK55 pKa = 9.61 CRR57 pKa = 11.84 DD58 pKa = 3.33 NMLSNPIDD66 pKa = 3.78 DD67 pKa = 4.48 TGSPTTPGPVTMTGGNTYY85 pKa = 11.14 AFIFSPSARR94 pKa = 11.84 VAGHH98 pKa = 6.88 EE99 pKa = 4.08 ISSGDD104 pKa = 3.51 RR105 pKa = 11.84 VNQPSSSQRR114 pKa = 11.84 RR115 pKa = 11.84 KK116 pKa = 9.89 SKK118 pKa = 10.77 CFVSGYY124 pKa = 10.74 GEE126 pKa = 4.28 TIEE129 pKa = 4.85 IQTDD133 pKa = 3.75 DD134 pKa = 3.46 ASPWVWRR141 pKa = 11.84 RR142 pKa = 11.84 IVFSTIGLAVQFQPGLLYY160 pKa = 11.26 NNDD163 pKa = 3.23 DD164 pKa = 3.33 TRR166 pKa = 11.84 GYY168 pKa = 11.15 EE169 pKa = 3.61 RR170 pKa = 11.84 TTFNLASGTAQADD183 pKa = 3.51 QLQGIVYY190 pKa = 9.62 RR191 pKa = 11.84 YY192 pKa = 9.63 LFQGSQDD199 pKa = 3.39 IDD201 pKa = 3.3 WANVMTANTAPRR213 pKa = 11.84 HH214 pKa = 4.91 VKK216 pKa = 9.99 VYY218 pKa = 9.32 SDD220 pKa = 3.57 RR221 pKa = 11.84 RR222 pKa = 11.84 RR223 pKa = 11.84 VIQSPNSEE231 pKa = 3.95 GGTIRR236 pKa = 11.84 ISKK239 pKa = 9.9 HH240 pKa = 4.87 YY241 pKa = 10.53 DD242 pKa = 3.08 SIRR245 pKa = 11.84 RR246 pKa = 11.84 SIIYY250 pKa = 10.14 DD251 pKa = 4.07 DD252 pKa = 4.12 IEE254 pKa = 5.7 AGSGAKK260 pKa = 8.39 ATNAIASGTPYY271 pKa = 11.37 GNMGDD276 pKa = 4.24 LFVMDD281 pKa = 5.11 FFSSASDD288 pKa = 4.04 DD289 pKa = 3.89 EE290 pKa = 4.71 TSGATFQPHH299 pKa = 4.72 GRR301 pKa = 11.84 YY302 pKa = 8.68 YY303 pKa = 8.44 WHH305 pKa = 7.46 EE306 pKa = 4.17 GQAVV310 pKa = 3.08
Molecular weight: 35.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.867
IPC_protein 10.921
Toseland 10.613
ProMoST 10.657
Dawson 10.789
Bjellqvist 10.643
Wikipedia 11.125
Rodwell 10.701
Grimsley 10.877
Solomon 10.979
Lehninger 10.906
Nozaki 10.613
DTASelect 10.643
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.73
Patrickios 10.262
IPC_peptide 10.965
IPC2_peptide 9.867
IPC2.peptide.svr19 8.372
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
635
310
325
317.5
36.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.614 ± 0.112
1.26 ± 0.429
7.402 ± 0.888
3.622 ± 0.726
6.299 ± 0.794
8.031 ± 0.202
2.677 ± 0.742
5.197 ± 0.425
3.307 ± 0.957
5.039 ± 1.49
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.417 ± 0.586
4.094 ± 0.519
4.409 ± 0.299
3.937 ± 0.179
11.339 ± 1.541
8.189 ± 1.713
5.984 ± 1.001
4.252 ± 0.634
2.205 ± 0.638
4.724 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here