Tamlana fucoidanivorans
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3334 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C4SP99|A0A5C4SP99_9FLAO (2R 3S)-2-methylisocitrate dehydratase OS=Tamlana fucoidanivorans OX=2583814 GN=FGF67_04835 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.24 KK3 pKa = 9.74 ILNICMMVITIIALNSCEE21 pKa = 4.35 EE22 pKa = 4.21 KK23 pKa = 10.23 DD24 pKa = 3.4 TSEE27 pKa = 5.74 FIAQEE32 pKa = 3.85 ATDD35 pKa = 4.16 FTFSTSFLEE44 pKa = 5.21 NYY46 pKa = 9.92 LLNNSLGNNLAEE58 pKa = 4.13 RR59 pKa = 11.84 FTWRR63 pKa = 11.84 DD64 pKa = 3.05 ANFGIPTEE72 pKa = 4.16 RR73 pKa = 11.84 TYY75 pKa = 11.31 EE76 pKa = 3.95 LQAATMEE83 pKa = 4.68 DD84 pKa = 3.62 FSDD87 pKa = 3.92 YY88 pKa = 11.44 TNDD91 pKa = 4.48 PDD93 pKa = 4.25 DD94 pKa = 4.93 SNYY97 pKa = 10.5 KK98 pKa = 10.38 AYY100 pKa = 11.06 NLGTNIGGNEE110 pKa = 3.48 IPATVKK116 pKa = 10.5 QFLALAKK123 pKa = 10.22 IAGLDD128 pKa = 3.72 NDD130 pKa = 5.1 PEE132 pKa = 4.53 TPAPNTGKK140 pKa = 10.75 LYY142 pKa = 10.33 FRR144 pKa = 11.84 VRR146 pKa = 11.84 ATIGKK151 pKa = 9.68 DD152 pKa = 3.05 NLEE155 pKa = 4.24 SFSAIQALNIEE166 pKa = 4.26 LQEE169 pKa = 4.39 GEE171 pKa = 4.55 EE172 pKa = 4.25 EE173 pKa = 4.38 VGGPNCPSWWVVGAGAPDD191 pKa = 4.91 AGWNWDD197 pKa = 3.46 NPIEE201 pKa = 4.39 FLCTDD206 pKa = 3.84 QIYY209 pKa = 9.17 TSNINLANDD218 pKa = 3.44 SFRR221 pKa = 11.84 FFTEE225 pKa = 3.29 KK226 pKa = 11.07 DD227 pKa = 3.12 NWDD230 pKa = 3.48 SGLNFPYY237 pKa = 10.21 FVDD240 pKa = 3.24 EE241 pKa = 5.55 GYY243 pKa = 10.93 SIDD246 pKa = 3.94 PNFEE250 pKa = 3.63 NAMDD254 pKa = 4.06 GDD256 pKa = 4.07 EE257 pKa = 4.2 NFRR260 pKa = 11.84 FIGTPGKK267 pKa = 10.7 YY268 pKa = 9.95 SLTIDD273 pKa = 4.22 SVNKK277 pKa = 9.1 TIKK280 pKa = 10.28 LGPPAAEE287 pKa = 5.14 DD288 pKa = 4.16 PNCDD292 pKa = 3.75 SVWVVGAGAVDD303 pKa = 5.03 AGWNWDD309 pKa = 3.45 SPIEE313 pKa = 4.12 FFCTDD318 pKa = 2.9 NVYY321 pKa = 9.3 STNIKK326 pKa = 8.59 LTNDD330 pKa = 2.84 AFRR333 pKa = 11.84 FFLEE337 pKa = 4.0 EE338 pKa = 4.39 GNWDD342 pKa = 3.38 SGQNYY347 pKa = 8.66 PFYY350 pKa = 11.24 ANDD353 pKa = 4.49 GYY355 pKa = 9.72 TIDD358 pKa = 5.08 SLLEE362 pKa = 4.09 DD363 pKa = 4.72 AQDD366 pKa = 3.5 GDD368 pKa = 4.45 NNFKK372 pKa = 11.15 FNGTPGTYY380 pKa = 10.55 LLTVDD385 pKa = 4.58 TVNKK389 pKa = 9.4 TITLGIPRR397 pKa = 11.84 PEE399 pKa = 4.34 EE400 pKa = 4.03 PNCDD404 pKa = 3.93 SVWVVGAGAVDD415 pKa = 5.03 AGWNWDD421 pKa = 3.49 SPIEE425 pKa = 4.14 FTCTDD430 pKa = 3.32 NVYY433 pKa = 10.48 SASINLTNDD442 pKa = 2.6 AFRR445 pKa = 11.84 FFLEE449 pKa = 4.0 EE450 pKa = 4.39 GNWDD454 pKa = 3.38 SGQNYY459 pKa = 8.63 PFYY462 pKa = 11.07 ADD464 pKa = 4.17 DD465 pKa = 5.48 GYY467 pKa = 9.64 TIDD470 pKa = 4.64 TLLEE474 pKa = 4.25 DD475 pKa = 5.14 AQDD478 pKa = 3.52 GDD480 pKa = 4.45 NNFKK484 pKa = 11.14 FNGTPGIYY492 pKa = 10.04 YY493 pKa = 7.49 FTVDD497 pKa = 3.94 TVNKK501 pKa = 10.23 IIDD504 pKa = 3.56 LRR506 pKa = 11.84 QQ507 pKa = 3.01
Molecular weight: 56.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.554
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.465
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.605
EMBOSS 3.706
Sillero 3.897
Patrickios 1.875
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|A0A5C4SJ00|A0A5C4SJ00_9FLAO RNA polymerase sigma factor OS=Tamlana fucoidanivorans OX=2583814 GN=FGF67_11925 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.27 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.09 GRR40 pKa = 11.84 KK41 pKa = 7.97 KK42 pKa = 10.62 LSVSSEE48 pKa = 4.03 TRR50 pKa = 11.84 HH51 pKa = 5.96 KK52 pKa = 10.68 KK53 pKa = 9.8
Molecular weight: 6.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3334
0
3334
1203641
24
4605
361.0
40.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.291 ± 0.038
0.803 ± 0.014
5.655 ± 0.031
6.311 ± 0.042
5.217 ± 0.035
6.469 ± 0.042
2.031 ± 0.022
7.749 ± 0.037
7.791 ± 0.048
9.124 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.148 ± 0.019
6.308 ± 0.043
3.498 ± 0.025
3.351 ± 0.021
3.435 ± 0.028
6.42 ± 0.033
5.711 ± 0.044
6.301 ± 0.033
1.188 ± 0.016
4.198 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here