Sphaerotilus natans subsp. natans DSM 6575

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Sphaerotilus; Sphaerotilus natans; Sphaerotilus natans subsp. natans

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4177 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059KK43|A0A059KK43_9BURK Uncharacterized protein OS=Sphaerotilus natans subsp. natans DSM 6575 OX=1286631 GN=X805_26800 PE=4 SV=1
MM1 pKa = 7.63LIEE4 pKa = 4.4PRR6 pKa = 11.84QPKK9 pKa = 9.4YY10 pKa = 10.17PWGLEE15 pKa = 3.41VRR17 pKa = 11.84AAIDD21 pKa = 4.09LYY23 pKa = 11.58NDD25 pKa = 3.59GSLPDD30 pKa = 3.56IDD32 pKa = 5.25EE33 pKa = 4.66DD34 pKa = 3.95QLLIAAGGPGEE45 pKa = 4.04IVQIGHH51 pKa = 5.8HH52 pKa = 6.06TEE54 pKa = 3.96ADD56 pKa = 3.53LPLYY60 pKa = 9.88MVDD63 pKa = 5.97FGLCVLGCLEE73 pKa = 4.21EE74 pKa = 4.81EE75 pKa = 4.63IVPSDD80 pKa = 4.19LPLPAPAPEE89 pKa = 4.38PEE91 pKa = 4.25PVGEE95 pKa = 4.23DD96 pKa = 3.22SARR99 pKa = 3.35

Molecular weight: 10.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059KKT5|A0A059KKT5_9BURK Periplasmic-binding protein OS=Sphaerotilus natans subsp. natans DSM 6575 OX=1286631 GN=X805_23460 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4177

0

4177

1379019

37

3862

330.1

35.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.448 ± 0.058

0.969 ± 0.013

5.466 ± 0.027

5.439 ± 0.032

3.086 ± 0.024

8.544 ± 0.039

2.403 ± 0.021

3.976 ± 0.028

2.378 ± 0.035

11.386 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.018

1.996 ± 0.022

5.669 ± 0.035

4.027 ± 0.024

8.16 ± 0.044

5.085 ± 0.035

4.942 ± 0.035

7.403 ± 0.027

1.505 ± 0.019

1.761 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski