Amino acid dipepetide frequency for Sphaerotilus natans subsp. natans DSM 6575

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.152AlaAla: 20.152 ± 0.202	1.434AlaCys: 1.434 ± 0.035	7.036AlaAsp: 7.036 ± 0.085	7.789AlaGlu: 7.789 ± 0.093	3.633AlaPhe: 3.633 ± 0.058	12.124AlaGly: 12.124 ± 0.11	2.78AlaHis: 2.78 ± 0.047	4.51AlaIle: 4.51 ± 0.057	2.69AlaLys: 2.69 ± 0.057	16.813AlaLeu: 16.813 ± 0.175	3.431AlaMet: 3.431 ± 0.058	1.997AlaAsn: 1.997 ± 0.041	7.316AlaPro: 7.316 ± 0.112	6.001AlaGln: 6.001 ± 0.086	11.049AlaArg: 11.049 ± 0.121	6.57AlaSer: 6.57 ± 0.088	5.991AlaThr: 5.991 ± 0.079	9.112AlaVal: 9.112 ± 0.09	2.094AlaTrp: 2.094 ± 0.044	1.955AlaTyr: 1.955 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.255CysAla: 1.255 ± 0.031	0.138CysCys: 0.138 ± 0.012	0.568CysAsp: 0.568 ± 0.021	0.541CysGlu: 0.541 ± 0.02	0.26CysPhe: 0.26 ± 0.014	1.088CysGly: 1.088 ± 0.033	0.298CysHis: 0.298 ± 0.012	0.389CysIle: 0.389 ± 0.016	0.166CysLys: 0.166 ± 0.012	0.847CysLeu: 0.847 ± 0.021	0.199CysMet: 0.199 ± 0.013	0.196CysAsn: 0.196 ± 0.011	0.563CysPro: 0.563 ± 0.023	0.302CysGln: 0.302 ± 0.016	0.814CysArg: 0.814 ± 0.027	0.542CysSer: 0.542 ± 0.022	0.552CysThr: 0.552 ± 0.021	0.66CysVal: 0.66 ± 0.023	0.131CysTrp: 0.131 ± 0.01	0.176CysTyr: 0.176 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.632AspAla: 7.632 ± 0.086	0.53AspCys: 0.53 ± 0.02	2.981AspAsp: 2.981 ± 0.057	3.556AspGlu: 3.556 ± 0.056	2.007AspPhe: 2.007 ± 0.039	5.001AspGly: 5.001 ± 0.075	1.246AspHis: 1.246 ± 0.036	2.254AspIle: 2.254 ± 0.04	1.267AspLys: 1.267 ± 0.036	6.275AspLeu: 6.275 ± 0.063	1.091AspMet: 1.091 ± 0.031	0.965AspAsn: 0.965 ± 0.025	3.446AspPro: 3.446 ± 0.053	1.658AspGln: 1.658 ± 0.035	4.162AspArg: 4.162 ± 0.058	2.257AspSer: 2.257 ± 0.042	2.704AspThr: 2.704 ± 0.067	3.389AspVal: 3.389 ± 0.052	1.099AspTrp: 1.099 ± 0.029	1.143AspTyr: 1.143 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	7.964GluAla: 7.964 ± 0.09	0.399GluCys: 0.399 ± 0.018	2.306GluAsp: 2.306 ± 0.05	2.286GluGlu: 2.286 ± 0.049	1.641GluPhe: 1.641 ± 0.037	3.899GluGly: 3.899 ± 0.056	1.436GluHis: 1.436 ± 0.031	2.784GluIle: 2.784 ± 0.057	1.376GluLys: 1.376 ± 0.048	5.983GluLeu: 5.983 ± 0.081	1.282GluMet: 1.282 ± 0.032	1.057GluAsn: 1.057 ± 0.031	2.878GluPro: 2.878 ± 0.052	2.653GluGln: 2.653 ± 0.053	5.74GluArg: 5.74 ± 0.076	2.309GluSer: 2.309 ± 0.043	2.645GluThr: 2.645 ± 0.048	4.323GluVal: 4.323 ± 0.064	0.634GluTrp: 0.634 ± 0.023	0.794GluTyr: 0.794 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.631PheAla: 3.631 ± 0.057	0.341PheCys: 0.341 ± 0.016	2.326PheAsp: 2.326 ± 0.043	1.99PheGlu: 1.99 ± 0.036	1.083PhePhe: 1.083 ± 0.032	3.157PheGly: 3.157 ± 0.054	0.739PheHis: 0.739 ± 0.027	1.225PheIle: 1.225 ± 0.034	0.798PheLys: 0.798 ± 0.029	2.723PheLeu: 2.723 ± 0.052	0.718PheMet: 0.718 ± 0.026	0.815PheAsn: 0.815 ± 0.024	1.311PhePro: 1.311 ± 0.027	0.955PheGln: 0.955 ± 0.029	1.92PheArg: 1.92 ± 0.037	1.875PheSer: 1.875 ± 0.039	1.585PheThr: 1.585 ± 0.033	2.534PheVal: 2.534 ± 0.044	0.466PheTrp: 0.466 ± 0.019	0.669PheTyr: 0.669 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	10.011GlyAla: 10.011 ± 0.111	1.048GlyCys: 1.048 ± 0.03	4.22GlyAsp: 4.22 ± 0.062	4.753GlyGlu: 4.753 ± 0.062	2.937GlyPhe: 2.937 ± 0.04	7.214GlyGly: 7.214 ± 0.092	2.206GlyHis: 2.206 ± 0.044	3.364GlyIle: 3.364 ± 0.052	2.16GlyLys: 2.16 ± 0.051	10.208GlyLeu: 10.208 ± 0.107	2.109GlyMet: 2.109 ± 0.041	1.674GlyAsn: 1.674 ± 0.059	3.529GlyPro: 3.529 ± 0.056	3.64GlyGln: 3.64 ± 0.06	7.4GlyArg: 7.4 ± 0.088	4.682GlySer: 4.682 ± 0.076	4.405GlyThr: 4.405 ± 0.088	6.389GlyVal: 6.389 ± 0.076	1.648GlyTrp: 1.648 ± 0.035	1.84GlyTyr: 1.84 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	3.171HisAla: 3.171 ± 0.05	0.307HisCys: 0.307 ± 0.013	1.472HisAsp: 1.472 ± 0.033	1.281HisGlu: 1.281 ± 0.032	0.881HisPhe: 0.881 ± 0.027	2.421HisGly: 2.421 ± 0.047	0.781HisHis: 0.781 ± 0.026	0.81HisIle: 0.81 ± 0.022	0.426HisLys: 0.426 ± 0.021	2.634HisLeu: 2.634 ± 0.048	0.427HisMet: 0.427 ± 0.016	0.424HisAsn: 0.424 ± 0.02	1.691HisPro: 1.691 ± 0.038	0.906HisGln: 0.906 ± 0.028	2.013HisArg: 2.013 ± 0.049	0.898HisSer: 0.898 ± 0.026	0.917HisThr: 0.917 ± 0.029	1.605HisVal: 1.605 ± 0.039	0.439HisTrp: 0.439 ± 0.019	0.528HisTyr: 0.528 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.664IleAla: 5.664 ± 0.069	0.378IleCys: 0.378 ± 0.017	3.432IleAsp: 3.432 ± 0.06	3.25IleGlu: 3.25 ± 0.066	1.073IlePhe: 1.073 ± 0.032	4.18IleGly: 4.18 ± 0.057	0.877IleHis: 0.877 ± 0.023	1.266IleIle: 1.266 ± 0.039	1.02IleLys: 1.02 ± 0.033	2.91IleLeu: 2.91 ± 0.044	0.595IleMet: 0.595 ± 0.023	1.068IleAsn: 1.068 ± 0.03	1.612IlePro: 1.612 ± 0.038	1.114IleGln: 1.114 ± 0.032	2.759IleArg: 2.759 ± 0.049	2.057IleSer: 2.057 ± 0.046	2.12IleThr: 2.12 ± 0.048	3.255IleVal: 3.255 ± 0.049	0.413IleTrp: 0.413 ± 0.019	0.721IleTyr: 0.721 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	3.064LysAla: 3.064 ± 0.065	0.117LysCys: 0.117 ± 0.01	1.281LysAsp: 1.281 ± 0.038	1.067LysGlu: 1.067 ± 0.036	0.698LysPhe: 0.698 ± 0.023	1.884LysGly: 1.884 ± 0.049	0.429LysHis: 0.429 ± 0.017	1.181LysIle: 1.181 ± 0.041	1.002LysLys: 1.002 ± 0.039	2.458LysLeu: 2.458 ± 0.05	0.618LysMet: 0.618 ± 0.025	0.685LysAsn: 0.685 ± 0.026	1.383LysPro: 1.383 ± 0.035	0.807LysGln: 0.807 ± 0.03	1.565LysArg: 1.565 ± 0.046	1.335LysSer: 1.335 ± 0.038	1.428LysThr: 1.428 ± 0.04	2.054LysVal: 2.054 ± 0.051	0.241LysTrp: 0.241 ± 0.014	0.489LysTyr: 0.489 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	16.386LeuAla: 16.386 ± 0.17	1.068LeuCys: 1.068 ± 0.029	6.595LeuAsp: 6.595 ± 0.075	5.592LeuGlu: 5.592 ± 0.078	3.041LeuPhe: 3.041 ± 0.052	9.433LeuGly: 9.433 ± 0.103	2.758LeuHis: 2.758 ± 0.047	4.251LeuIle: 4.251 ± 0.065	3.051LeuLys: 3.051 ± 0.06	12.579LeuLeu: 12.579 ± 0.168	2.769LeuMet: 2.769 ± 0.044	2.204LeuAsn: 2.204 ± 0.039	7.094LeuPro: 7.094 ± 0.087	4.763LeuGln: 4.763 ± 0.083	9.365LeuArg: 9.365 ± 0.097	6.296LeuSer: 6.296 ± 0.083	5.373LeuThr: 5.373 ± 0.064	7.97LeuVal: 7.97 ± 0.083	1.5LeuTrp: 1.5 ± 0.041	1.771LeuTyr: 1.771 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	2.935MetAla: 2.935 ± 0.045	0.139MetCys: 0.139 ± 0.009	1.043MetAsp: 1.043 ± 0.028	0.898MetGlu: 0.898 ± 0.025	0.608MetPhe: 0.608 ± 0.02	1.558MetGly: 1.558 ± 0.039	0.538MetHis: 0.538 ± 0.02	1.001MetIle: 1.001 ± 0.03	0.842MetLys: 0.842 ± 0.029	2.816MetLeu: 2.816 ± 0.046	0.609MetMet: 0.609 ± 0.021	0.824MetAsn: 0.824 ± 0.025	1.562MetPro: 1.562 ± 0.033	1.018MetGln: 1.018 ± 0.029	1.875MetArg: 1.875 ± 0.036	1.73MetSer: 1.73 ± 0.034	1.545MetThr: 1.545 ± 0.036	1.579MetVal: 1.579 ± 0.036	0.171MetTrp: 0.171 ± 0.011	0.296MetTyr: 0.296 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.532AsnAla: 2.532 ± 0.046	0.246AsnCys: 0.246 ± 0.018	1.085AsnAsp: 1.085 ± 0.03	0.919AsnGlu: 0.919 ± 0.028	0.705AsnPhe: 0.705 ± 0.025	1.665AsnGly: 1.665 ± 0.035	0.455AsnHis: 0.455 ± 0.017	0.871AsnIle: 0.871 ± 0.027	0.534AsnLys: 0.534 ± 0.02	2.252AsnLeu: 2.252 ± 0.05	0.421AsnMet: 0.421 ± 0.017	0.462AsnAsn: 0.462 ± 0.022	1.482AsnPro: 1.482 ± 0.034	0.695AsnGln: 0.695 ± 0.024	1.535AsnArg: 1.535 ± 0.032	0.874AsnSer: 0.874 ± 0.029	1.039AsnThr: 1.039 ± 0.029	1.412AsnVal: 1.412 ± 0.04	0.297AsnTrp: 0.297 ± 0.017	0.476AsnTyr: 0.476 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	8.849ProAla: 8.849 ± 0.106	0.434ProCys: 0.434 ± 0.018	3.693ProAsp: 3.693 ± 0.053	3.734ProGlu: 3.734 ± 0.058	1.578ProPhe: 1.578 ± 0.038	5.283ProGly: 5.283 ± 0.072	1.146ProHis: 1.146 ± 0.028	1.78ProIle: 1.78 ± 0.04	1.287ProLys: 1.287 ± 0.035	5.577ProLeu: 5.577 ± 0.073	1.384ProMet: 1.384 ± 0.034	1.009ProAsn: 1.009 ± 0.029	3.082ProPro: 3.082 ± 0.061	2.207ProGln: 2.207 ± 0.042	3.563ProArg: 3.563 ± 0.065	2.706ProSer: 2.706 ± 0.042	2.61ProThr: 2.61 ± 0.052	5.02ProVal: 5.02 ± 0.078	0.824ProTrp: 0.824 ± 0.025	0.922ProTyr: 0.922 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	6.548GlnAla: 6.548 ± 0.094	0.289GlnCys: 0.289 ± 0.014	1.755GlnAsp: 1.755 ± 0.043	1.473GlnGlu: 1.473 ± 0.04	1.123GlnPhe: 1.123 ± 0.029	3.099GlnGly: 3.099 ± 0.053	1.027GlnHis: 1.027 ± 0.031	1.86GlnIle: 1.86 ± 0.045	0.856GlnLys: 0.856 ± 0.026	4.196GlnLeu: 4.196 ± 0.066	0.978GlnMet: 0.978 ± 0.027	0.634GlnAsn: 0.634 ± 0.024	2.616GlnPro: 2.616 ± 0.051	1.975GlnGln: 1.975 ± 0.048	3.843GlnArg: 3.843 ± 0.094	1.677GlnSer: 1.677 ± 0.039	1.816GlnThr: 1.816 ± 0.039	3.356GlnVal: 3.356 ± 0.052	0.578GlnTrp: 0.578 ± 0.019	0.568GlnTyr: 0.568 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	9.486ArgAla: 9.486 ± 0.106	0.764ArgCys: 0.764 ± 0.024	4.139ArgAsp: 4.139 ± 0.058	4.903ArgGlu: 4.903 ± 0.073	2.739ArgPhe: 2.739 ± 0.042	5.473ArgGly: 5.473 ± 0.085	2.642ArgHis: 2.642 ± 0.046	3.729ArgIle: 3.729 ± 0.058	1.554ArgLys: 1.554 ± 0.039	10.736ArgLeu: 10.736 ± 0.131	2.017ArgMet: 2.017 ± 0.042	1.413ArgAsn: 1.413 ± 0.032	4.569ArgPro: 4.569 ± 0.07	3.918ArgGln: 3.918 ± 0.068	7.38ArgArg: 7.38 ± 0.094	3.838ArgSer: 3.838 ± 0.063	3.63ArgThr: 3.63 ± 0.051	5.588ArgVal: 5.588 ± 0.071	1.54ArgTrp: 1.54 ± 0.034	1.542ArgTyr: 1.542 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	6.643SerAla: 6.643 ± 0.081	0.484SerCys: 0.484 ± 0.021	2.518SerAsp: 2.518 ± 0.046	2.26SerGlu: 2.26 ± 0.047	1.793SerPhe: 1.793 ± 0.046	5.37SerGly: 5.37 ± 0.081	1.141SerHis: 1.141 ± 0.031	2.02SerIle: 2.02 ± 0.043	1.102SerLys: 1.102 ± 0.033	5.294SerLeu: 5.294 ± 0.077	1.211SerMet: 1.211 ± 0.033	1.016SerAsn: 1.016 ± 0.034	2.977SerPro: 2.977 ± 0.05	1.627SerGln: 1.627 ± 0.035	4.046SerArg: 4.046 ± 0.056	3.036SerSer: 3.036 ± 0.068	2.86SerThr: 2.86 ± 0.061	3.776SerVal: 3.776 ± 0.066	0.714SerTrp: 0.714 ± 0.02	0.964SerTyr: 0.964 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	5.727ThrAla: 5.727 ± 0.067	0.453ThrCys: 0.453 ± 0.021	2.537ThrAsp: 2.537 ± 0.049	2.381ThrGlu: 2.381 ± 0.049	1.478ThrPhe: 1.478 ± 0.035	4.671ThrGly: 4.671 ± 0.077	1.038ThrHis: 1.038 ± 0.027	1.889ThrIle: 1.889 ± 0.057	0.939ThrLys: 0.939 ± 0.029	6.687ThrLeu: 6.687 ± 0.088	1.099ThrMet: 1.099 ± 0.033	0.963ThrAsn: 0.963 ± 0.031	3.638ThrPro: 3.638 ± 0.056	1.665ThrGln: 1.665 ± 0.037	3.556ThrArg: 3.556 ± 0.051	2.429ThrSer: 2.429 ± 0.046	2.713ThrThr: 2.713 ± 0.054	4.067ThrVal: 4.067 ± 0.067	0.65ThrTrp: 0.65 ± 0.023	0.834ThrTyr: 0.834 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	9.339ValAla: 9.339 ± 0.096	0.745ValCys: 0.745 ± 0.024	4.096ValAsp: 4.096 ± 0.061	4.358ValGlu: 4.358 ± 0.065	2.376ValPhe: 2.376 ± 0.045	5.425ValGly: 5.425 ± 0.069	1.676ValHis: 1.676 ± 0.032	3.26ValIle: 3.26 ± 0.053	1.86ValLys: 1.86 ± 0.051	8.75ValLeu: 8.75 ± 0.092	1.896ValMet: 1.896 ± 0.036	1.709ValAsn: 1.709 ± 0.041	4.154ValPro: 4.154 ± 0.057	2.832ValGln: 2.832 ± 0.044	5.814ValArg: 5.814 ± 0.062	3.942ValSer: 3.942 ± 0.066	3.752ValThr: 3.752 ± 0.063	5.754ValVal: 5.754 ± 0.081	1.037ValTrp: 1.037 ± 0.031	1.25ValTyr: 1.25 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.427TrpAla: 1.427 ± 0.033	0.19TrpCys: 0.19 ± 0.01	0.601TrpAsp: 0.601 ± 0.022	0.49TrpGlu: 0.49 ± 0.021	0.507TrpPhe: 0.507 ± 0.018	0.925TrpGly: 0.925 ± 0.029	0.406TrpHis: 0.406 ± 0.018	0.699TrpIle: 0.699 ± 0.022	0.394TrpLys: 0.394 ± 0.017	2.171TrpLeu: 2.171 ± 0.04	0.423TrpMet: 0.423 ± 0.018	0.404TrpAsn: 0.404 ± 0.018	0.892TrpPro: 0.892 ± 0.029	0.837TrpGln: 0.837 ± 0.025	1.586TrpArg: 1.586 ± 0.039	0.869TrpSer: 0.869 ± 0.029	0.744TrpThr: 0.744 ± 0.023	0.965TrpVal: 0.965 ± 0.027	0.28TrpTrp: 0.28 ± 0.016	0.238TrpTyr: 0.238 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.059TyrAla: 2.059 ± 0.037	0.182TyrCys: 0.182 ± 0.012	0.981TyrAsp: 0.981 ± 0.026	0.867TyrGlu: 0.867 ± 0.027	0.701TyrPhe: 0.701 ± 0.023	1.568TyrGly: 1.568 ± 0.036	0.413TyrHis: 0.413 ± 0.018	0.62TyrIle: 0.62 ± 0.026	0.46TyrLys: 0.46 ± 0.021	1.948TyrLeu: 1.948 ± 0.035	0.31TyrMet: 0.31 ± 0.015	0.437TyrAsn: 0.437 ± 0.018	0.893TyrPro: 0.893 ± 0.027	0.695TyrGln: 0.695 ± 0.023	1.614TyrArg: 1.614 ± 0.035	0.931TyrSer: 0.931 ± 0.028	0.987TyrThr: 0.987 ± 0.028	1.217TyrVal: 1.217 ± 0.031	0.293TyrTrp: 0.293 ± 0.014	0.43TyrTyr: 0.43 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4177 proteins (1379019 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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