Helicobacter sanguini
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2259 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A347VQB1|A0A347VQB1_9HELI Uncharacterized protein OS=Helicobacter sanguini OX=1548018 GN=DCO61_09435 PE=4 SV=1
MM1 pKa = 8.11 RR2 pKa = 11.84 DD3 pKa = 3.1 NVIYY7 pKa = 11.11 GEE9 pKa = 4.14 FVKK12 pKa = 10.78 AALQDD17 pKa = 3.47 RR18 pKa = 11.84 LDD20 pKa = 3.78 LVRR23 pKa = 11.84 EE24 pKa = 4.2 RR25 pKa = 11.84 GWEE28 pKa = 3.81 FATCDD33 pKa = 3.37 MEE35 pKa = 5.35 EE36 pKa = 4.21 YY37 pKa = 10.77 ALEE40 pKa = 4.1 LLAEE44 pKa = 4.68 CGVDD48 pKa = 5.14 ADD50 pKa = 4.77 DD51 pKa = 4.54 TPSGIIDD58 pKa = 3.32 NALINGCHH66 pKa = 6.77 GYY68 pKa = 9.51 LANYY72 pKa = 9.42 SGYY75 pKa = 10.91 EE76 pKa = 3.89 NLQEE80 pKa = 4.85 AMEE83 pKa = 4.58 AYY85 pKa = 9.42 EE86 pKa = 4.44 KK87 pKa = 11.61 GEE89 pKa = 4.37 LIYY92 pKa = 11.08 LNIPNEE98 pKa = 3.92 SRR100 pKa = 11.84 ARR102 pKa = 11.84 EE103 pKa = 4.09 LIKK106 pKa = 10.56 QQEE109 pKa = 4.47 TLQSQINDD117 pKa = 3.29 IEE119 pKa = 4.35 NEE121 pKa = 4.31 TFGEE125 pKa = 4.15 EE126 pKa = 3.66 LTAEE130 pKa = 4.18 QEE132 pKa = 4.23 AEE134 pKa = 4.2 LNEE137 pKa = 4.02 LTAEE141 pKa = 4.2 LKK143 pKa = 10.61 DD144 pKa = 3.8 VQEE147 pKa = 4.62 KK148 pKa = 11.2 LNDD151 pKa = 3.26 IYY153 pKa = 11.56 NEE155 pKa = 4.11 SVYY158 pKa = 10.57 IKK160 pKa = 10.75 YY161 pKa = 10.54 LL162 pKa = 3.5
Molecular weight: 18.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.826
IPC2_protein 4.037
IPC_protein 3.973
Toseland 3.808
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.77
Rodwell 3.808
Grimsley 3.719
Solomon 3.91
Lehninger 3.859
Nozaki 4.024
DTASelect 4.139
Thurlkill 3.821
EMBOSS 3.795
Sillero 4.075
Patrickios 1.875
IPC_peptide 3.91
IPC2_peptide 4.062
IPC2.peptide.svr19 3.963
Protein with the highest isoelectric point:
>tr|A0A099BC60|A0A099BC60_9HELI Methionine aminopeptidase OS=Helicobacter sanguini OX=1548018 GN=map PE=3 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.32 RR14 pKa = 11.84 THH16 pKa = 6.11 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 8.44 TKK25 pKa = 10.16 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.41 GRR39 pKa = 11.84 KK40 pKa = 8.41 RR41 pKa = 11.84 LSVAA45 pKa = 3.33
Molecular weight: 5.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2259
0
2259
738759
26
2885
327.0
37.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.225 ± 0.054
1.114 ± 0.021
5.679 ± 0.042
6.118 ± 0.05
5.411 ± 0.048
5.413 ± 0.058
1.651 ± 0.02
8.799 ± 0.05
8.834 ± 0.072
9.733 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.237 ± 0.023
7.176 ± 0.072
2.784 ± 0.028
3.277 ± 0.032
3.397 ± 0.033
8.077 ± 0.051
4.471 ± 0.037
5.062 ± 0.051
0.656 ± 0.015
3.887 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here