Clostridium paraputrificum

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3219 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B8RSZ1|A0A1B8RSZ1_9CLOT Uncharacterized protein OS=Clostridium paraputrificum OX=29363 GN=CP373A1_03245 PE=4 SV=1
MM1 pKa = 7.68EE2 pKa = 5.47NNNFYY7 pKa = 11.15NLTDD11 pKa = 3.76EE12 pKa = 5.04DD13 pKa = 4.86VEE15 pKa = 4.47TLRR18 pKa = 11.84LLDD21 pKa = 3.69LQLYY25 pKa = 8.91GNLVFIVSDD34 pKa = 3.49VLSYY38 pKa = 11.0ISTIEE43 pKa = 4.38SIDD46 pKa = 3.73LVYY49 pKa = 10.9SKK51 pKa = 11.56YY52 pKa = 11.08EE53 pKa = 3.89NTSEE57 pKa = 4.14NLPDD61 pKa = 4.75PDD63 pKa = 3.91VPALEE68 pKa = 4.22SAYY71 pKa = 10.37LAVIARR77 pKa = 11.84SILFDD82 pKa = 3.16IGFARR87 pKa = 11.84YY88 pKa = 8.4GHH90 pKa = 6.46LVEE93 pKa = 5.04KK94 pKa = 10.32FNNGEE99 pKa = 3.87IDD101 pKa = 3.92FSLSPDD107 pKa = 3.2EE108 pKa = 6.26DD109 pKa = 3.74INLGNIFGMISYY121 pKa = 8.6MYY123 pKa = 9.99FLRR126 pKa = 11.84GVMGIYY132 pKa = 10.31EE133 pKa = 4.91RR134 pKa = 11.84DD135 pKa = 3.04LSLPVFGLL143 pKa = 3.63

Molecular weight: 16.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A174I8N8|A0A174I8N8_9CLOT Transcriptional regulator OS=Clostridium paraputrificum OX=29363 GN=CP373A1_13415 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.2DD3 pKa = 3.06VRR5 pKa = 11.84RR6 pKa = 11.84PNGKK10 pKa = 7.97MRR12 pKa = 11.84RR13 pKa = 11.84SKK15 pKa = 10.83RR16 pKa = 11.84KK17 pKa = 8.42VCAFCVDD24 pKa = 3.31KK25 pKa = 11.49AEE27 pKa = 4.63SIDD30 pKa = 3.78YY31 pKa = 10.84KK32 pKa = 11.2DD33 pKa = 3.39VAKK36 pKa = 10.51LRR38 pKa = 11.84KK39 pKa = 9.29YY40 pKa = 8.08VTEE43 pKa = 4.22RR44 pKa = 11.84GKK46 pKa = 10.15ILPRR50 pKa = 11.84RR51 pKa = 11.84ISGTCAKK58 pKa = 9.97HH59 pKa = 5.6QRR61 pKa = 11.84QLTDD65 pKa = 4.2AIKK68 pKa = 10.26RR69 pKa = 11.84SRR71 pKa = 11.84NIALLPFTTEE81 pKa = 3.59

Molecular weight: 9.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3219

0

3219

1008064

37

2795

313.2

35.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.434 ± 0.044

1.16 ± 0.017

5.67 ± 0.033

7.738 ± 0.047

4.359 ± 0.029

6.751 ± 0.042

1.28 ± 0.015

9.797 ± 0.05

8.831 ± 0.039

9.14 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.021

6.209 ± 0.042

2.746 ± 0.027

2.037 ± 0.02

3.432 ± 0.025

6.399 ± 0.04

4.801 ± 0.035

6.775 ± 0.034

0.67 ± 0.011

4.196 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski