Amino acid dipepetide frequency for Clostridium paraputrificum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.46AlaAla: 3.46 ± 0.079	0.689AlaCys: 0.689 ± 0.031	2.479AlaAsp: 2.479 ± 0.051	3.266AlaGlu: 3.266 ± 0.071	2.492AlaPhe: 2.492 ± 0.051	3.734AlaGly: 3.734 ± 0.081	0.777AlaHis: 0.777 ± 0.029	5.747AlaIle: 5.747 ± 0.087	4.413AlaLys: 4.413 ± 0.079	6.033AlaLeu: 6.033 ± 0.087	1.586AlaMet: 1.586 ± 0.041	2.585AlaAsn: 2.585 ± 0.057	1.463AlaPro: 1.463 ± 0.039	1.33AlaGln: 1.33 ± 0.037	1.806AlaArg: 1.806 ± 0.044	3.331AlaSer: 3.331 ± 0.052	2.866AlaThr: 2.866 ± 0.053	3.801AlaVal: 3.801 ± 0.072	0.361AlaTrp: 0.361 ± 0.019	2.116AlaTyr: 2.116 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.543CysAla: 0.543 ± 0.023	0.191CysCys: 0.191 ± 0.019	0.685CysAsp: 0.685 ± 0.028	0.778CysGlu: 0.778 ± 0.03	0.496CysPhe: 0.496 ± 0.021	1.09CysGly: 1.09 ± 0.039	0.197CysHis: 0.197 ± 0.013	1.101CysIle: 1.101 ± 0.035	1.031CysLys: 1.031 ± 0.032	0.849CysLeu: 0.849 ± 0.031	0.254CysMet: 0.254 ± 0.016	0.765CysAsn: 0.765 ± 0.027	0.441CysPro: 0.441 ± 0.021	0.186CysGln: 0.186 ± 0.013	0.412CysArg: 0.412 ± 0.023	0.792CysSer: 0.792 ± 0.029	0.587CysThr: 0.587 ± 0.024	0.677CysVal: 0.677 ± 0.025	0.066CysTrp: 0.066 ± 0.008	0.454CysTyr: 0.454 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	2.595AspAla: 2.595 ± 0.058	0.582AspCys: 0.582 ± 0.024	2.806AspAsp: 2.806 ± 0.055	5.104AspGlu: 5.104 ± 0.079	2.784AspPhe: 2.784 ± 0.058	3.838AspGly: 3.838 ± 0.066	0.556AspHis: 0.556 ± 0.023	6.22AspIle: 6.22 ± 0.086	5.542AspLys: 5.542 ± 0.078	5.001AspLeu: 5.001 ± 0.073	1.549AspMet: 1.549 ± 0.041	3.453AspAsn: 3.453 ± 0.075	1.295AspPro: 1.295 ± 0.034	0.722AspGln: 0.722 ± 0.027	2.019AspArg: 2.019 ± 0.043	3.367AspSer: 3.367 ± 0.062	2.512AspThr: 2.512 ± 0.047	3.711AspVal: 3.711 ± 0.066	0.356AspTrp: 0.356 ± 0.022	2.692AspTyr: 2.692 ± 0.052	0.0AspXaa: 0.0 ± 0.0
Glu	4.243GluAla: 4.243 ± 0.08	0.804GluCys: 0.804 ± 0.027	4.825GluAsp: 4.825 ± 0.078	8.123GluGlu: 8.123 ± 0.123	3.133GluPhe: 3.133 ± 0.054	4.968GluGly: 4.968 ± 0.066	0.895GluHis: 0.895 ± 0.03	7.042GluIle: 7.042 ± 0.094	7.425GluLys: 7.425 ± 0.106	7.137GluLeu: 7.137 ± 0.097	1.989GluMet: 1.989 ± 0.042	4.962GluAsn: 4.962 ± 0.085	1.462GluPro: 1.462 ± 0.036	1.477GluGln: 1.477 ± 0.037	2.724GluArg: 2.724 ± 0.058	3.762GluSer: 3.762 ± 0.069	2.818GluThr: 2.818 ± 0.054	5.809GluVal: 5.809 ± 0.1	0.49GluTrp: 0.49 ± 0.022	3.288GluTyr: 3.288 ± 0.064	0.0GluXaa: 0.0 ± 0.0
Phe	2.147PheAla: 2.147 ± 0.053	0.494PheCys: 0.494 ± 0.022	2.459PheAsp: 2.459 ± 0.049	2.592PheGlu: 2.592 ± 0.056	1.952PhePhe: 1.952 ± 0.051	3.046PheGly: 3.046 ± 0.068	0.519PheHis: 0.519 ± 0.024	4.813PheIle: 4.813 ± 0.08	3.812PheLys: 3.812 ± 0.067	3.909PheLeu: 3.909 ± 0.078	1.211PheMet: 1.211 ± 0.035	3.029PheAsn: 3.029 ± 0.062	1.114PhePro: 1.114 ± 0.034	0.889PheGln: 0.889 ± 0.031	1.277PheArg: 1.277 ± 0.036	3.135PheSer: 3.135 ± 0.064	2.336PheThr: 2.336 ± 0.055	2.764PheVal: 2.764 ± 0.052	0.274PheTrp: 0.274 ± 0.014	1.815PheTyr: 1.815 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	4.275GlyAla: 4.275 ± 0.089	0.925GlyCys: 0.925 ± 0.037	3.581GlyAsp: 3.581 ± 0.064	4.919GlyGlu: 4.919 ± 0.08	3.066GlyPhe: 3.066 ± 0.063	4.594GlyGly: 4.594 ± 0.078	0.95GlyHis: 0.95 ± 0.034	7.06GlyIle: 7.06 ± 0.094	5.808GlyLys: 5.808 ± 0.076	5.848GlyLeu: 5.848 ± 0.081	1.839GlyMet: 1.839 ± 0.046	3.481GlyAsn: 3.481 ± 0.063	1.256GlyPro: 1.256 ± 0.039	1.405GlyGln: 1.405 ± 0.041	2.399GlyArg: 2.399 ± 0.057	3.786GlySer: 3.786 ± 0.071	3.41GlyThr: 3.41 ± 0.066	5.314GlyVal: 5.314 ± 0.089	0.544GlyTrp: 0.544 ± 0.027	3.052GlyTyr: 3.052 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	0.618HisAla: 0.618 ± 0.026	0.206HisCys: 0.206 ± 0.016	0.682HisAsp: 0.682 ± 0.026	0.806HisGlu: 0.806 ± 0.029	0.566HisPhe: 0.566 ± 0.023	0.985HisGly: 0.985 ± 0.033	0.273HisHis: 0.273 ± 0.019	1.243HisIle: 1.243 ± 0.035	1.006HisLys: 1.006 ± 0.028	1.131HisLeu: 1.131 ± 0.033	0.299HisMet: 0.299 ± 0.016	0.805HisAsn: 0.805 ± 0.029	0.572HisPro: 0.572 ± 0.026	0.249HisGln: 0.249 ± 0.016	0.516HisArg: 0.516 ± 0.022	0.824HisSer: 0.824 ± 0.03	0.662HisThr: 0.662 ± 0.028	0.697HisVal: 0.697 ± 0.025	0.097HisTrp: 0.097 ± 0.009	0.568HisTyr: 0.568 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.493IleAla: 5.493 ± 0.085	1.195IleCys: 1.195 ± 0.04	5.902IleAsp: 5.902 ± 0.087	7.238IleGlu: 7.238 ± 0.095	4.182IlePhe: 4.182 ± 0.076	6.284IleGly: 6.284 ± 0.089	1.213IleHis: 1.213 ± 0.037	9.189IleIle: 9.189 ± 0.137	8.73IleLys: 8.73 ± 0.096	9.141IleLeu: 9.141 ± 0.129	2.294IleMet: 2.294 ± 0.047	6.352IleAsn: 6.352 ± 0.094	3.265IlePro: 3.265 ± 0.054	1.986IleGln: 1.986 ± 0.043	3.176IleArg: 3.176 ± 0.064	7.034IleSer: 7.034 ± 0.096	4.838IleThr: 4.838 ± 0.066	6.35IleVal: 6.35 ± 0.093	0.505IleTrp: 0.505 ± 0.026	3.603IleTyr: 3.603 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	4.603LysAla: 4.603 ± 0.072	0.861LysCys: 0.861 ± 0.03	6.153LysAsp: 6.153 ± 0.087	9.435LysGlu: 9.435 ± 0.131	3.199LysPhe: 3.199 ± 0.058	5.382LysGly: 5.382 ± 0.075	1.0LysHis: 1.0 ± 0.033	7.534LysIle: 7.534 ± 0.092	8.075LysLys: 8.075 ± 0.114	7.342LysLeu: 7.342 ± 0.084	2.261LysMet: 2.261 ± 0.039	5.737LysAsn: 5.737 ± 0.079	1.819LysPro: 1.819 ± 0.043	1.648LysGln: 1.648 ± 0.041	3.21LysArg: 3.21 ± 0.065	5.047LysSer: 5.047 ± 0.074	3.652LysThr: 3.652 ± 0.057	6.588LysVal: 6.588 ± 0.094	0.605LysTrp: 0.605 ± 0.025	4.157LysTyr: 4.157 ± 0.06	0.0LysXaa: 0.0 ± 0.0
Leu	5.264LeuAla: 5.264 ± 0.09	1.121LeuCys: 1.121 ± 0.038	5.449LeuAsp: 5.449 ± 0.074	6.496LeuGlu: 6.496 ± 0.092	3.828LeuPhe: 3.828 ± 0.075	6.456LeuGly: 6.456 ± 0.086	1.071LeuHis: 1.071 ± 0.034	8.423LeuIle: 8.423 ± 0.11	8.018LeuLys: 8.018 ± 0.101	8.104LeuLeu: 8.104 ± 0.125	2.428LeuMet: 2.428 ± 0.052	5.687LeuAsn: 5.687 ± 0.073	2.806LeuPro: 2.806 ± 0.054	1.939LeuGln: 1.939 ± 0.041	3.132LeuArg: 3.132 ± 0.06	6.935LeuSer: 6.935 ± 0.09	4.396LeuThr: 4.396 ± 0.069	5.892LeuVal: 5.892 ± 0.072	0.562LeuTrp: 0.562 ± 0.025	3.387LeuTyr: 3.387 ± 0.062	0.0LeuXaa: 0.0 ± 0.0
Met	1.685MetAla: 1.685 ± 0.042	0.281MetCys: 0.281 ± 0.017	1.537MetAsp: 1.537 ± 0.04	1.766MetGlu: 1.766 ± 0.04	1.065MetPhe: 1.065 ± 0.034	1.798MetGly: 1.798 ± 0.044	0.352MetHis: 0.352 ± 0.019	2.259MetIle: 2.259 ± 0.054	2.51MetLys: 2.51 ± 0.045	2.165MetLeu: 2.165 ± 0.049	0.657MetMet: 0.657 ± 0.026	1.745MetAsn: 1.745 ± 0.041	0.896MetPro: 0.896 ± 0.035	0.607MetGln: 0.607 ± 0.023	0.89MetArg: 0.89 ± 0.029	1.685MetSer: 1.685 ± 0.042	1.149MetThr: 1.149 ± 0.035	1.696MetVal: 1.696 ± 0.041	0.172MetTrp: 0.172 ± 0.013	0.854MetTyr: 0.854 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	2.813AsnAla: 2.813 ± 0.061	0.654AsnCys: 0.654 ± 0.028	3.124AsnAsp: 3.124 ± 0.072	4.529AsnGlu: 4.529 ± 0.076	2.524AsnPhe: 2.524 ± 0.052	4.04AsnGly: 4.04 ± 0.072	0.775AsnHis: 0.775 ± 0.027	6.906AsnIle: 6.906 ± 0.092	6.088AsnLys: 6.088 ± 0.089	5.498AsnLeu: 5.498 ± 0.081	1.591AsnMet: 1.591 ± 0.039	4.567AsnAsn: 4.567 ± 0.082	2.161AsnPro: 2.161 ± 0.047	1.126AsnGln: 1.126 ± 0.036	2.076AsnArg: 2.076 ± 0.042	4.018AsnSer: 4.018 ± 0.075	2.857AsnThr: 2.857 ± 0.059	3.56AsnVal: 3.56 ± 0.065	0.416AsnTrp: 0.416 ± 0.02	2.765AsnTyr: 2.765 ± 0.049	0.0AsnXaa: 0.0 ± 0.0
Pro	1.355ProAla: 1.355 ± 0.039	0.306ProCys: 0.306 ± 0.018	1.356ProAsp: 1.356 ± 0.038	2.156ProGlu: 2.156 ± 0.052	1.404ProPhe: 1.404 ± 0.032	1.621ProGly: 1.621 ± 0.046	0.444ProHis: 0.444 ± 0.022	2.75ProIle: 2.75 ± 0.061	2.068ProLys: 2.068 ± 0.049	2.528ProLeu: 2.528 ± 0.053	0.715ProMet: 0.715 ± 0.025	1.691ProAsn: 1.691 ± 0.044	0.551ProPro: 0.551 ± 0.027	0.702ProGln: 0.702 ± 0.027	0.794ProArg: 0.794 ± 0.03	1.874ProSer: 1.874 ± 0.048	1.588ProThr: 1.588 ± 0.037	1.977ProVal: 1.977 ± 0.048	0.251ProTrp: 0.251 ± 0.019	1.327ProTyr: 1.327 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	1.18GlnAla: 1.18 ± 0.033	0.245GlnCys: 0.245 ± 0.014	1.06GlnAsp: 1.06 ± 0.032	1.546GlnGlu: 1.546 ± 0.038	0.789GlnPhe: 0.789 ± 0.026	1.495GlnGly: 1.495 ± 0.038	0.255GlnHis: 0.255 ± 0.018	1.864GlnIle: 1.864 ± 0.04	1.692GlnLys: 1.692 ± 0.046	1.873GlnLeu: 1.873 ± 0.04	0.556GlnMet: 0.556 ± 0.024	1.185GlnAsn: 1.185 ± 0.039	0.467GlnPro: 0.467 ± 0.024	0.548GlnGln: 0.548 ± 0.026	0.839GlnArg: 0.839 ± 0.033	1.23GlnSer: 1.23 ± 0.033	0.934GlnThr: 0.934 ± 0.033	1.485GlnVal: 1.485 ± 0.04	0.197GlnTrp: 0.197 ± 0.014	0.928GlnTyr: 0.928 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	1.858ArgAla: 1.858 ± 0.042	0.418ArgCys: 0.418 ± 0.022	2.004ArgAsp: 2.004 ± 0.043	3.209ArgGlu: 3.209 ± 0.062	1.521ArgPhe: 1.521 ± 0.04	2.222ArgGly: 2.222 ± 0.048	0.453ArgHis: 0.453 ± 0.023	3.229ArgIle: 3.229 ± 0.057	2.861ArgLys: 2.861 ± 0.059	2.962ArgLeu: 2.962 ± 0.056	0.987ArgMet: 0.987 ± 0.033	1.989ArgAsn: 1.989 ± 0.046	0.785ArgPro: 0.785 ± 0.029	0.733ArgGln: 0.733 ± 0.027	1.576ArgArg: 1.576 ± 0.045	1.646ArgSer: 1.646 ± 0.036	1.498ArgThr: 1.498 ± 0.037	2.544ArgVal: 2.544 ± 0.05	0.24ArgTrp: 0.24 ± 0.016	1.584ArgTyr: 1.584 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	3.181SerAla: 3.181 ± 0.06	0.658SerCys: 0.658 ± 0.027	3.32SerAsp: 3.32 ± 0.065	4.086SerGlu: 4.086 ± 0.066	3.13SerPhe: 3.13 ± 0.058	4.422SerGly: 4.422 ± 0.076	0.834SerHis: 0.834 ± 0.03	6.686SerIle: 6.686 ± 0.09	5.767SerLys: 5.767 ± 0.09	6.195SerLeu: 6.195 ± 0.087	1.59SerMet: 1.59 ± 0.041	4.158SerAsn: 4.158 ± 0.081	1.69SerPro: 1.69 ± 0.043	1.47SerGln: 1.47 ± 0.037	2.085SerArg: 2.085 ± 0.05	4.368SerSer: 4.368 ± 0.076	3.301SerThr: 3.301 ± 0.061	3.819SerVal: 3.819 ± 0.061	0.454SerTrp: 0.454 ± 0.023	2.775SerTyr: 2.775 ± 0.055	0.0SerXaa: 0.0 ± 0.0
Thr	2.659ThrAla: 2.659 ± 0.055	0.508ThrCys: 0.508 ± 0.026	2.321ThrAsp: 2.321 ± 0.042	2.868ThrGlu: 2.868 ± 0.054	2.253ThrPhe: 2.253 ± 0.048	3.533ThrGly: 3.533 ± 0.06	0.737ThrHis: 0.737 ± 0.023	4.787ThrIle: 4.787 ± 0.071	3.45ThrLys: 3.45 ± 0.051	5.059ThrLeu: 5.059 ± 0.072	1.108ThrMet: 1.108 ± 0.035	2.75ThrAsn: 2.75 ± 0.061	1.866ThrPro: 1.866 ± 0.044	1.045ThrGln: 1.045 ± 0.034	1.513ThrArg: 1.513 ± 0.037	3.329ThrSer: 3.329 ± 0.067	2.679ThrThr: 2.679 ± 0.056	3.273ThrVal: 3.273 ± 0.064	0.343ThrTrp: 0.343 ± 0.021	1.93ThrTyr: 1.93 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	4.129ValAla: 4.129 ± 0.073	0.848ValCys: 0.848 ± 0.029	4.062ValAsp: 4.062 ± 0.072	5.002ValGlu: 5.002 ± 0.083	2.867ValPhe: 2.867 ± 0.069	4.708ValGly: 4.708 ± 0.078	0.866ValHis: 0.866 ± 0.029	6.507ValIle: 6.507 ± 0.101	5.642ValLys: 5.642 ± 0.085	6.196ValLeu: 6.196 ± 0.081	1.697ValMet: 1.697 ± 0.044	3.888ValAsn: 3.888 ± 0.069	2.084ValPro: 2.084 ± 0.05	1.397ValGln: 1.397 ± 0.034	2.113ValArg: 2.113 ± 0.046	4.423ValSer: 4.423 ± 0.072	3.499ValThr: 3.499 ± 0.067	5.013ValVal: 5.013 ± 0.085	0.385ValTrp: 0.385 ± 0.017	2.427ValTyr: 2.427 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	0.341TrpAla: 0.341 ± 0.02	0.087TrpCys: 0.087 ± 0.009	0.428TrpAsp: 0.428 ± 0.024	0.419TrpGlu: 0.419 ± 0.02	0.298TrpPhe: 0.298 ± 0.016	0.536TrpGly: 0.536 ± 0.024	0.105TrpHis: 0.105 ± 0.01	0.635TrpIle: 0.635 ± 0.028	0.526TrpLys: 0.526 ± 0.023	0.558TrpLeu: 0.558 ± 0.026	0.182TrpMet: 0.182 ± 0.015	0.44TrpAsn: 0.44 ± 0.02	0.163TrpPro: 0.163 ± 0.012	0.178TrpGln: 0.178 ± 0.012	0.259TrpArg: 0.259 ± 0.018	0.44TrpSer: 0.44 ± 0.024	0.338TrpThr: 0.338 ± 0.023	0.389TrpVal: 0.389 ± 0.023	0.071TrpTrp: 0.071 ± 0.009	0.309TrpTyr: 0.309 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.896TyrAla: 1.896 ± 0.043	0.523TyrCys: 0.523 ± 0.021	2.468TyrAsp: 2.468 ± 0.047	3.041TyrGlu: 3.041 ± 0.055	2.04TyrPhe: 2.04 ± 0.046	2.76TyrGly: 2.76 ± 0.054	0.532TyrHis: 0.532 ± 0.022	3.976TyrIle: 3.976 ± 0.066	3.843TyrLys: 3.843 ± 0.059	3.868TyrLeu: 3.868 ± 0.061	0.973TyrMet: 0.973 ± 0.03	2.818TyrAsn: 2.818 ± 0.052	1.302TyrPro: 1.302 ± 0.039	0.733TyrGln: 0.733 ± 0.025	1.504TyrArg: 1.504 ± 0.04	2.965TyrSer: 2.965 ± 0.059	2.091TyrThr: 2.091 ± 0.054	2.392TyrVal: 2.392 ± 0.053	0.31TyrTrp: 0.31 ± 0.02	1.922TyrTyr: 1.922 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3219 proteins (1008064 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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