Hankyongella ginsenosidimutans
Average proteome isoelectric point is 7.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1977 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D7C9E4|A0A4D7C9E4_9SPHN Uncharacterized protein OS=Hankyongella ginsenosidimutans OX=1763828 GN=E6W36_08015 PE=4 SV=1
MM1 pKa = 7.81 ANGLSSAVSTLTFTLQDD18 pKa = 2.9 TDD20 pKa = 3.36 EE21 pKa = 4.59 RR22 pKa = 11.84 LNPVIASTPVPVAEE36 pKa = 4.38 NTTGTTIAALSGGSDD51 pKa = 3.71 PDD53 pKa = 4.0 GDD55 pKa = 4.04 PQEE58 pKa = 3.84 IDD60 pKa = 3.43 YY61 pKa = 10.81 FFVTGYY67 pKa = 11.1 DD68 pKa = 3.12 PGGLLRR74 pKa = 11.84 IDD76 pKa = 3.62 QTATGANLVVATAFDD91 pKa = 4.19 YY92 pKa = 11.26 EE93 pKa = 4.76 SASQLYY99 pKa = 9.3 PGQFYY104 pKa = 9.12 YY105 pKa = 10.85 TDD107 pKa = 3.09 AGGRR111 pKa = 11.84 YY112 pKa = 8.89 VEE114 pKa = 4.28 AKK116 pKa = 10.11 VLAVQATNSNGFGSDD131 pKa = 3.32 ADD133 pKa = 3.47 NSTVNRR139 pKa = 11.84 SPATTIKK146 pKa = 10.81 VYY148 pKa = 8.99 VTNADD153 pKa = 4.01 DD154 pKa = 5.05 APGAPSAQNINVSEE168 pKa = 4.12 NVTGVIATLSGGIDD182 pKa = 3.38 QDD184 pKa = 3.35 GDD186 pKa = 3.82 AISYY190 pKa = 8.44 VFSNGSTTYY199 pKa = 10.91 GDD201 pKa = 3.62 YY202 pKa = 10.77 TISEE206 pKa = 4.69 DD207 pKa = 3.68 YY208 pKa = 10.52 KK209 pKa = 11.33 LSITRR214 pKa = 11.84 AFDD217 pKa = 3.87 YY218 pKa = 10.98 EE219 pKa = 4.41 SLPANNKK226 pKa = 6.4 STSVAVYY233 pKa = 10.09 SKK235 pKa = 11.68 ANGKK239 pKa = 9.53 ISANSANITLNITNANDD256 pKa = 3.25 APYY259 pKa = 10.86 VYY261 pKa = 9.03 MGPSTISIKK270 pKa = 10.76 EE271 pKa = 4.0 NIPINTVIAEE281 pKa = 3.95 FTINDD286 pKa = 3.61 QDD288 pKa = 3.67 NDD290 pKa = 4.1 NITTTLGTSVLNNLLGLKK308 pKa = 9.34 PKK310 pKa = 10.19 AAIRR314 pKa = 11.84 IFINCTT320 pKa = 3.01
Molecular weight: 33.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.91
IPC_protein 3.923
Toseland 3.694
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.872
Rodwell 3.745
Grimsley 3.592
Solomon 3.91
Lehninger 3.872
Nozaki 4.024
DTASelect 4.304
Thurlkill 3.745
EMBOSS 3.872
Sillero 4.037
Patrickios 1.189
IPC_peptide 3.91
IPC2_peptide 4.012
IPC2.peptide.svr19 3.927
Protein with the highest isoelectric point:
>tr|A0A4D7C5M3|A0A4D7C5M3_9SPHN Uncharacterized protein OS=Hankyongella ginsenosidimutans OX=1763828 GN=E6W36_03330 PE=4 SV=1
MM1 pKa = 7.44 AVSSVQVAITILLVTTWFILAVWALFRR28 pKa = 11.84 SRR30 pKa = 11.84 RR31 pKa = 11.84 SSQRR35 pKa = 11.84 ARR37 pKa = 11.84 AARR40 pKa = 11.84 AWALRR45 pKa = 11.84 LRR47 pKa = 11.84 ALISTTPGAISWRR60 pKa = 11.84 RR61 pKa = 11.84 GMSPSVQVAARR72 pKa = 11.84 LAFAA76 pKa = 5.28
Molecular weight: 8.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.501
Grimsley 13.042
Solomon 13.495
Lehninger 13.393
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.237
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1977
0
1977
414989
24
1110
209.9
22.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.719 ± 0.088
1.125 ± 0.025
5.759 ± 0.054
4.605 ± 0.056
3.21 ± 0.037
8.384 ± 0.06
1.903 ± 0.032
4.497 ± 0.037
2.558 ± 0.049
10.29 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.205 ± 0.03
2.52 ± 0.041
5.545 ± 0.065
3.568 ± 0.038
8.514 ± 0.09
5.785 ± 0.056
5.504 ± 0.056
6.9 ± 0.053
1.397 ± 0.028
2.011 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here