Candidatus Regiella insecticola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; aphid secondary symbionts; Candidatus Regiella

Average proteome isoelectric point is 7.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1994 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L2ZPH8|A0A6L2ZPH8_9ENTR Uncharacterized protein OS=Candidatus Regiella insecticola OX=138073 GN=RINTU1_20350 PE=4 SV=1
MM1 pKa = 7.71SILCFLPPYY10 pKa = 10.13FEE12 pKa = 5.27SIYY15 pKa = 10.61ILLALRR21 pKa = 11.84SEE23 pKa = 4.84GVFSVVDD30 pKa = 3.72AVGSCSLPALDD41 pKa = 4.79INEE44 pKa = 4.72LGADD48 pKa = 3.63SFIEE52 pKa = 4.28GFVFIVSDD60 pKa = 4.0FLIFGCVNVGLSACCTGLTKK80 pKa = 10.61CCSLEE85 pKa = 4.24TFLGTDD91 pKa = 4.84DD92 pKa = 4.25IVSACSCWLFSDD104 pKa = 4.42PVVVPCGAVIKK115 pKa = 10.88VEE117 pKa = 4.15LFTIVIANGALFVVGRR133 pKa = 11.84TGG135 pKa = 3.18

Molecular weight: 14.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L2ZM52|A0A6L2ZM52_9ENTR Antitoxin PemI OS=Candidatus Regiella insecticola OX=138073 GN=pemI PE=4 SV=1
MM1 pKa = 7.52NGSQYY6 pKa = 10.52NRR8 pKa = 11.84RR9 pKa = 11.84LRR11 pKa = 11.84PVILGKK17 pKa = 10.03IVMKK21 pKa = 10.03RR22 pKa = 11.84TFQPSVLKK30 pKa = 10.6RR31 pKa = 11.84NRR33 pKa = 11.84SHH35 pKa = 7.16GFRR38 pKa = 11.84ARR40 pKa = 11.84MATKK44 pKa = 10.4NGRR47 pKa = 11.84QVLARR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84AKK56 pKa = 10.22SRR58 pKa = 11.84ARR60 pKa = 11.84LTVSKK65 pKa = 10.99

Molecular weight: 7.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1994

0

1994

515872

32

1503

258.7

29.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.2 ± 0.052

1.283 ± 0.022

4.995 ± 0.04

5.586 ± 0.055

3.879 ± 0.034

6.228 ± 0.052

2.426 ± 0.031

7.0 ± 0.049

6.203 ± 0.057

10.87 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.028

4.448 ± 0.036

4.071 ± 0.035

4.692 ± 0.058

5.758 ± 0.049

6.07 ± 0.043

5.399 ± 0.043

6.141 ± 0.046

1.194 ± 0.024

2.939 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski