Aquimarina aggregata
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5047 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A163BYT3|A0A163BYT3_9FLAO Uncharacterized protein OS=Aquimarina aggregata OX=1642818 GN=AWE51_00215 PE=4 SV=1
MM1 pKa = 7.52 FLQLLIILNFKK12 pKa = 10.25 LMKK15 pKa = 9.2 QLFYY19 pKa = 10.94 IFILLLGFTSCEE31 pKa = 3.8 DD32 pKa = 3.75 VIDD35 pKa = 3.74 VDD37 pKa = 4.27 VPNGEE42 pKa = 3.92 PRR44 pKa = 11.84 LVVDD48 pKa = 5.14 ASFEE52 pKa = 4.35 VYY54 pKa = 10.61 LNEE57 pKa = 4.6 VPVTVKK63 pKa = 10.91 NSIQLSLSAPFFGNTIPKK81 pKa = 10.08 VNDD84 pKa = 3.07 ATVFITDD91 pKa = 4.63 LSDD94 pKa = 3.31 GTIINYY100 pKa = 8.74 TEE102 pKa = 4.03 TNGTGFYY109 pKa = 10.18 IPSSTFLPEE118 pKa = 3.85 FNVDD122 pKa = 3.45 YY123 pKa = 10.43 EE124 pKa = 4.08 LTIIYY129 pKa = 10.52 NSEE132 pKa = 4.01 TYY134 pKa = 10.52 KK135 pKa = 10.53 ATTQMFASVPIDD147 pKa = 3.89 NIEE150 pKa = 4.12 QGDD153 pKa = 3.96 GTLFEE158 pKa = 4.95 GDD160 pKa = 3.26 EE161 pKa = 4.41 TEE163 pKa = 4.73 IIVSFTDD170 pKa = 3.46 DD171 pKa = 3.2 GARR174 pKa = 11.84 DD175 pKa = 3.78 DD176 pKa = 5.55 FYY178 pKa = 11.62 LFNFDD183 pKa = 4.26 FDD185 pKa = 4.28 LLEE188 pKa = 4.15 VSEE191 pKa = 4.29 DD192 pKa = 2.95 RR193 pKa = 11.84 FYY195 pKa = 11.17 QGEE198 pKa = 4.5 SFNFSYY204 pKa = 10.16 FYY206 pKa = 11.24 EE207 pKa = 4.73 DD208 pKa = 2.89 VTAGQNITIKK218 pKa = 10.54 ILGIDD223 pKa = 3.21 KK224 pKa = 10.83 RR225 pKa = 11.84 FFNYY229 pKa = 10.35 AGLLIDD235 pKa = 5.12 QSDD238 pKa = 4.12 PDD240 pKa = 3.9 GGGPFATPPALLRR253 pKa = 11.84 GNVINTTNSDD263 pKa = 3.63 NYY265 pKa = 11.1 ALGYY269 pKa = 10.65 FNLSEE274 pKa = 4.48 ANQLDD279 pKa = 3.91 FTIQEE284 pKa = 4.32 KK285 pKa = 10.71
Molecular weight: 32.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.694
IPC_protein 3.706
Toseland 3.49
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.63
Rodwell 3.528
Grimsley 3.401
Solomon 3.694
Lehninger 3.643
Nozaki 3.808
DTASelect 4.037
Thurlkill 3.541
EMBOSS 3.643
Sillero 3.821
Patrickios 1.125
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.757
Protein with the highest isoelectric point:
>tr|A0A162XJN4|A0A162XJN4_9FLAO HTH luxR-type domain-containing protein OS=Aquimarina aggregata OX=1642818 GN=AWE51_13885 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 10.15 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 KK13 pKa = 8.29 NKK15 pKa = 9.34 HH16 pKa = 4.03 GFRR19 pKa = 11.84 EE20 pKa = 4.05 RR21 pKa = 11.84 MASVNGRR28 pKa = 11.84 KK29 pKa = 9.21 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.92 GRR39 pKa = 11.84 KK40 pKa = 8.34 KK41 pKa = 10.22 ISVSSEE47 pKa = 3.56 LRR49 pKa = 11.84 HH50 pKa = 5.43 KK51 pKa = 10.29 HH52 pKa = 5.04
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5047
0
5047
1774003
49
4566
351.5
39.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.826 ± 0.036
0.736 ± 0.011
5.738 ± 0.03
6.38 ± 0.032
5.187 ± 0.028
6.385 ± 0.035
1.772 ± 0.016
8.365 ± 0.038
7.988 ± 0.051
8.989 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.95 ± 0.018
6.497 ± 0.039
3.352 ± 0.023
3.412 ± 0.019
3.417 ± 0.018
6.705 ± 0.031
6.168 ± 0.043
5.89 ± 0.026
1.112 ± 0.013
4.131 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here