Flammeovirga sp. MY04 
Average proteome isoelectric point is 6.11 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 5417 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A1B1G1I7|A0A1B1G1I7_9BACT Sulfatase-like hydrolase/transferase OS=Flammeovirga sp. MY04 OX=1191459 GN=MY04_4845 PE=4 SV=1 
MM1 pKa = 7.44  KK2 pKa = 10.56  LIARR6 pKa = 11.84  LATLLLLSVCLFSCDD21 pKa = 5.32  DD22 pKa = 5.31  DD23 pKa = 6.69  DD24 pKa = 7.34  DD25 pKa = 5.55  SDD27 pKa = 4.11  PRR29 pKa = 11.84  DD30 pKa = 3.36  VNYY33 pKa = 10.36  VYY35 pKa = 10.53  FVTDD39 pKa = 4.91  EE40 pKa = 4.08  IPAPDD45 pKa = 3.96  EE46 pKa = 4.58  FGDD49 pKa = 4.6  PNVQLQFNTSSNNIEE64 pKa = 4.08  VVIRR68 pKa = 11.84  TSVEE72 pKa = 3.25  IGTAATTVVAEE83 pKa = 4.19  YY84 pKa = 8.95  TQDD87 pKa = 3.13  VTSYY91 pKa = 11.24  NRR93 pKa = 11.84  DD94 pKa = 2.93  GDD96 pKa = 3.89  NYY98 pKa = 10.66  SIQIDD103 pKa = 3.56  SGSTYY108 pKa = 10.06  NQEE111 pKa = 3.67  LKK113 pKa = 10.91  ADD115 pKa = 4.05  VGCVSIPDD123 pKa = 3.77  SGFSMGKK130 pKa = 6.25  TTTSVSLDD138 pKa = 4.67  DD139 pKa = 3.57  ILVWDD144 pKa = 4.47  SSNSKK149 pKa = 10.7  YY150 pKa = 10.71  YY151 pKa = 10.21  IPGEE155 pKa = 4.02  KK156 pKa = 9.65  LGEE159 pKa = 4.02  LLGVVVDD166 pKa = 4.4  PEE168 pKa = 4.43  FGQGGGGNVVYY179 pKa = 9.6  PKK181 pKa = 9.69  IWSSDD186 pKa = 3.18  GSIMKK191 pKa = 10.42  CGEE194 pKa = 4.2  FPTNLPNN201 pKa = 4.15   
 Molecular weight: 21.78 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.688 
IPC2_protein 3.757 
IPC_protein 3.77 
Toseland    3.541 
ProMoST     3.948 
Dawson      3.77 
Bjellqvist  3.923 
Wikipedia   3.719 
Rodwell     3.592 
Grimsley    3.452 
Solomon     3.757 
Lehninger   3.719 
Nozaki      3.884 
DTASelect   4.151 
Thurlkill   3.605 
EMBOSS      3.732 
Sillero     3.897 
Patrickios  1.074 
IPC_peptide 3.757 
IPC2_peptide  3.859 
IPC2.peptide.svr19  3.774 
 Protein with the highest isoelectric point: 
>tr|A0A1B1FXI0|A0A1B1FXI0_9BACT 1 4-beta-xylanase OS=Flammeovirga sp. MY04 OX=1191459 GN=MY04_3522 PE=3 SV=1 
MM1 pKa = 7.45  KK2 pKa = 9.61  RR3 pKa = 11.84  TFQPSNRR10 pKa = 11.84  KK11 pKa = 9.07  RR12 pKa = 11.84  KK13 pKa = 8.41  NKK15 pKa = 9.86  HH16 pKa = 4.44  GFRR19 pKa = 11.84  QRR21 pKa = 11.84  MLTANGRR28 pKa = 11.84  NVLASRR34 pKa = 11.84  RR35 pKa = 11.84  KK36 pKa = 9.6  KK37 pKa = 10.04  GRR39 pKa = 11.84  KK40 pKa = 8.87  SLTVSDD46 pKa = 4.03  SRR48 pKa = 11.84  KK49 pKa = 10.05  HH50 pKa = 5.27  KK51 pKa = 10.79  AA52 pKa = 3.07   
 Molecular weight: 6.15 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.407 
IPC2_protein 10.877 
IPC_protein 12.442 
Toseland    12.618 
ProMoST     13.1 
Dawson      12.618 
Bjellqvist  12.603 
Wikipedia   13.086 
Rodwell     12.427 
Grimsley    12.661 
Solomon     13.115 
Lehninger   13.013 
Nozaki      12.618 
DTASelect   12.603 
Thurlkill   12.618 
EMBOSS      13.115 
Sillero     12.618 
Patrickios  12.149 
IPC_peptide 13.115 
IPC2_peptide  12.091 
IPC2.peptide.svr19  9.037 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        5416 
 
        
        1 
 
        
        5417 
         
        2124546
 
        36
 
        5547
 
        392.2
 
        44.41
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        5.794 ± 0.036
0.713 ± 0.009
 
        6.042 ± 0.026
6.929 ± 0.035
 
        5.069 ± 0.023
6.569 ± 0.036
 
        1.941 ± 0.014
7.494 ± 0.029
       
        7.479 ± 0.052
8.881 ± 0.035
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.355 ± 0.015
6.059 ± 0.032
 
        3.389 ± 0.021
3.68 ± 0.018
 
        3.361 ± 0.023
6.694 ± 0.029
 
        5.678 ± 0.041
6.303 ± 0.029
       
        1.219 ± 0.013
4.35 ± 0.021
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here