Flammeovirga sp. MY04
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5417 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B1G1I7|A0A1B1G1I7_9BACT Sulfatase-like hydrolase/transferase OS=Flammeovirga sp. MY04 OX=1191459 GN=MY04_4845 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.56 LIARR6 pKa = 11.84 LATLLLLSVCLFSCDD21 pKa = 5.32 DD22 pKa = 5.31 DD23 pKa = 6.69 DD24 pKa = 7.34 DD25 pKa = 5.55 SDD27 pKa = 4.11 PRR29 pKa = 11.84 DD30 pKa = 3.36 VNYY33 pKa = 10.36 VYY35 pKa = 10.53 FVTDD39 pKa = 4.91 EE40 pKa = 4.08 IPAPDD45 pKa = 3.96 EE46 pKa = 4.58 FGDD49 pKa = 4.6 PNVQLQFNTSSNNIEE64 pKa = 4.08 VVIRR68 pKa = 11.84 TSVEE72 pKa = 3.25 IGTAATTVVAEE83 pKa = 4.19 YY84 pKa = 8.95 TQDD87 pKa = 3.13 VTSYY91 pKa = 11.24 NRR93 pKa = 11.84 DD94 pKa = 2.93 GDD96 pKa = 3.89 NYY98 pKa = 10.66 SIQIDD103 pKa = 3.56 SGSTYY108 pKa = 10.06 NQEE111 pKa = 3.67 LKK113 pKa = 10.91 ADD115 pKa = 4.05 VGCVSIPDD123 pKa = 3.77 SGFSMGKK130 pKa = 6.25 TTTSVSLDD138 pKa = 4.67 DD139 pKa = 3.57 ILVWDD144 pKa = 4.47 SSNSKK149 pKa = 10.7 YY150 pKa = 10.71 YY151 pKa = 10.21 IPGEE155 pKa = 4.02 KK156 pKa = 9.65 LGEE159 pKa = 4.02 LLGVVVDD166 pKa = 4.4 PEE168 pKa = 4.43 FGQGGGGNVVYY179 pKa = 9.6 PKK181 pKa = 9.69 IWSSDD186 pKa = 3.18 GSIMKK191 pKa = 10.42 CGEE194 pKa = 4.2 FPTNLPNN201 pKa = 4.15
Molecular weight: 21.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.541
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.719
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.151
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.897
Patrickios 1.074
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.774
Protein with the highest isoelectric point:
>tr|A0A1B1FXI0|A0A1B1FXI0_9BACT 1 4-beta-xylanase OS=Flammeovirga sp. MY04 OX=1191459 GN=MY04_3522 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 KK11 pKa = 9.07 RR12 pKa = 11.84 KK13 pKa = 8.41 NKK15 pKa = 9.86 HH16 pKa = 4.44 GFRR19 pKa = 11.84 QRR21 pKa = 11.84 MLTANGRR28 pKa = 11.84 NVLASRR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.6 KK37 pKa = 10.04 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLTVSDD46 pKa = 4.03 SRR48 pKa = 11.84 KK49 pKa = 10.05 HH50 pKa = 5.27 KK51 pKa = 10.79 AA52 pKa = 3.07
Molecular weight: 6.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.407
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.427
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.149
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.037
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5416
1
5417
2124546
36
5547
392.2
44.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.794 ± 0.036
0.713 ± 0.009
6.042 ± 0.026
6.929 ± 0.035
5.069 ± 0.023
6.569 ± 0.036
1.941 ± 0.014
7.494 ± 0.029
7.479 ± 0.052
8.881 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.355 ± 0.015
6.059 ± 0.032
3.389 ± 0.021
3.68 ± 0.018
3.361 ± 0.023
6.694 ± 0.029
5.678 ± 0.041
6.303 ± 0.029
1.219 ± 0.013
4.35 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here