Sulfolobus monocaudavirus SMV2
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N7A9S6|A0A0N7A9S6_9VIRU MoxR-like ATPase OS=Sulfolobus monocaudavirus SMV2 OX=1580591 PE=4 SV=1
MM1 pKa = 7.21 SQSQKK6 pKa = 10.61 PNLYY10 pKa = 10.38 LLSLYY15 pKa = 9.24 PSPVGVTVTGDD26 pKa = 3.33 TTDD29 pKa = 3.29 QTVSFVISPYY39 pKa = 9.75 HH40 pKa = 5.69 YY41 pKa = 11.04 VDD43 pKa = 3.15 ISYY46 pKa = 10.73 LITSYY51 pKa = 11.63 NDD53 pKa = 3.33 GVQVYY58 pKa = 9.81 APDD61 pKa = 3.86 ANIPQSGISGFPPGVPTVTLIIEE84 pKa = 4.34 KK85 pKa = 10.73 GNLNFEE91 pKa = 4.39 FATPEE96 pKa = 4.97 DD97 pKa = 3.5 ILKK100 pKa = 10.65 SYY102 pKa = 10.45 LYY104 pKa = 11.24 NNILQNMNNDD114 pKa = 3.12 LTEE117 pKa = 4.54 IYY119 pKa = 10.22 QDD121 pKa = 4.81 LITLISEE128 pKa = 4.45 YY129 pKa = 11.18 ASGQSLSQSYY139 pKa = 10.34 LSEE142 pKa = 4.14 VSSLLQQINNMLQNAEE158 pKa = 4.08 QFEE161 pKa = 4.49 NTANLSFEE169 pKa = 4.41 GVSALQQVYY178 pKa = 11.1 NNLNEE183 pKa = 4.42 IYY185 pKa = 10.67 NSLTNQTLTLGEE197 pKa = 4.55 LEE199 pKa = 4.35 SLQLPSYY206 pKa = 7.54 STPEE210 pKa = 3.76 ASSIASNYY218 pKa = 7.33 TQFLNNAVSIMQNASSQSGSSSSSSQGSNSSSSTPQSSSSSNASSQSGSSSSPSPLSSLTTNSQSGTSSSSYY290 pKa = 8.96 NTQTSTSSTTTITTSTSTSSTTSQTSIISPPTLSQSQVLAQIQAGDD336 pKa = 3.69 VSSLLSEE343 pKa = 4.34 LSEE346 pKa = 4.45 IPSNMIPVVNTVVQLYY362 pKa = 9.36 QLYY365 pKa = 9.52 GFPDD369 pKa = 4.34 GTPLSTVVEE378 pKa = 4.23 RR379 pKa = 11.84 LLSDD383 pKa = 3.12 IRR385 pKa = 11.84 EE386 pKa = 3.99 AYY388 pKa = 9.85 YY389 pKa = 10.86 NIVNHH394 pKa = 5.84 KK395 pKa = 9.46 QEE397 pKa = 4.33 EE398 pKa = 4.53 VNLQQLSQLLQLYY411 pKa = 7.42 NTLVSNGLAPQSQSASKK428 pKa = 10.57 LSEE431 pKa = 3.87 LTQTSEE437 pKa = 5.04 IIPALPPVLGQPYY450 pKa = 9.16 PGRR453 pKa = 11.84 VNSNSNSNSVNYY465 pKa = 10.84 SNFII469 pKa = 3.77
Molecular weight: 50.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.946
IPC2_protein 3.923
IPC_protein 3.859
Toseland 3.681
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.694
Rodwell 3.694
Grimsley 3.605
Solomon 3.808
Lehninger 3.757
Nozaki 3.935
DTASelect 4.062
Thurlkill 3.706
EMBOSS 3.706
Sillero 3.973
Patrickios 0.006
IPC_peptide 3.808
IPC2_peptide 3.961
IPC2.peptide.svr19 3.897
Protein with the highest isoelectric point:
>tr|A0A0N7AA29|A0A0N7AA29_9VIRU Uncharacterized protein OS=Sulfolobus monocaudavirus SMV2 OX=1580591 PE=4 SV=1
MM1 pKa = 8.29 VEE3 pKa = 3.94 VKK5 pKa = 10.57 LRR7 pKa = 11.84 WRR9 pKa = 11.84 VKK11 pKa = 9.94 INRR14 pKa = 11.84 KK15 pKa = 8.62 NGKK18 pKa = 9.4 EE19 pKa = 3.65 YY20 pKa = 9.03 MLRR23 pKa = 11.84 YY24 pKa = 9.84 INVTSRR30 pKa = 11.84 SAVFLINYY38 pKa = 8.13 VPYY41 pKa = 10.69 FNIKK45 pKa = 10.32 NNIIEE50 pKa = 4.43 FKK52 pKa = 10.14 PNRR55 pKa = 11.84 RR56 pKa = 11.84 YY57 pKa = 10.0 AKK59 pKa = 9.15 KK60 pKa = 10.12 GLRR63 pKa = 11.84 IRR65 pKa = 11.84 LRR67 pKa = 11.84 WLIKK71 pKa = 10.0 RR72 pKa = 11.84 EE73 pKa = 3.97 KK74 pKa = 10.65 KK75 pKa = 9.57 NGKK78 pKa = 9.14 VFTQYY83 pKa = 10.79 YY84 pKa = 9.57 ISIPSEE90 pKa = 3.61 LAKK93 pKa = 10.46 PVVDD97 pKa = 4.91 LDD99 pKa = 4.14 PYY101 pKa = 10.93 LVPEE105 pKa = 3.94 NMQIVFRR112 pKa = 11.84 PKK114 pKa = 10.2 EE115 pKa = 3.92 GDD117 pKa = 3.54 GMGLNNQLFVDD128 pKa = 4.98 DD129 pKa = 4.64 EE130 pKa = 5.33 GEE132 pKa = 4.31 TQSQQ136 pKa = 3.19
Molecular weight: 16.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.162
IPC2_protein 9.502
IPC_protein 9.575
Toseland 10.145
ProMoST 9.809
Dawson 10.335
Bjellqvist 9.984
Wikipedia 10.496
Rodwell 10.789
Grimsley 10.409
Solomon 10.365
Lehninger 10.335
Nozaki 10.116
DTASelect 9.984
Thurlkill 10.189
EMBOSS 10.54
Sillero 10.233
Patrickios 10.438
IPC_peptide 10.365
IPC2_peptide 8.521
IPC2.peptide.svr19 8.564
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
65
0
65
14878
45
1563
228.9
25.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.807 ± 0.382
0.376 ± 0.085
4.315 ± 0.313
6.701 ± 0.672
4.463 ± 0.319
5.175 ± 0.318
0.921 ± 0.09
7.783 ± 0.309
6.466 ± 0.631
10.075 ± 0.333
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.124 ± 0.191
6.083 ± 0.399
4.241 ± 0.329
4.651 ± 0.657
3.32 ± 0.359
7.494 ± 0.582
5.767 ± 0.46
7.151 ± 0.395
0.86 ± 0.141
5.29 ± 0.363
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here