Alkaliphilus pronyensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Alkaliphilus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2908 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I0F7X3|A0A6I0F7X3_9CLOT DUF418 domain-containing protein OS=Alkaliphilus pronyensis OX=1482732 GN=F8154_00995 PE=4 SV=1
MM1 pKa = 7.9KK2 pKa = 10.5YY3 pKa = 10.19QDD5 pKa = 3.74GSNTVNGYY13 pKa = 9.19VVGVRR18 pKa = 11.84TGSALGDD25 pKa = 3.62AADD28 pKa = 4.33PTNDD32 pKa = 4.03GYY34 pKa = 11.71DD35 pKa = 3.24HH36 pKa = 6.98LRR38 pKa = 11.84NAEE41 pKa = 4.02HH42 pKa = 6.68VGTVIQDD49 pKa = 3.49ADD51 pKa = 3.65SDD53 pKa = 4.46AEE55 pKa = 4.18NPTPVLISIEE65 pKa = 4.11ITTLPTKK72 pKa = 10.05TNYY75 pKa = 10.4LVGEE79 pKa = 4.28SLDD82 pKa = 3.49LTGIVVTGSFSGGSSGTVDD101 pKa = 3.23VTVDD105 pKa = 3.52DD106 pKa = 3.84VTGFDD111 pKa = 3.9SSASAASQTLTVTVDD126 pKa = 2.89GCTDD130 pKa = 3.33TFDD133 pKa = 3.63VTISS137 pKa = 3.22

Molecular weight: 14.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I0FA05|A0A6I0FA05_9CLOT Ribosomal silencing factor RsfS OS=Alkaliphilus pronyensis OX=1482732 GN=rsfS PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.2KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.69EE15 pKa = 3.5HH16 pKa = 6.13GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MRR23 pKa = 11.84TSNGRR28 pKa = 11.84NIIKK32 pKa = 10.25RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.26GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2908

0

2908

869982

28

2848

299.2

33.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.038 ± 0.046

0.951 ± 0.018

5.258 ± 0.038

7.554 ± 0.061

4.128 ± 0.036

6.601 ± 0.046

1.643 ± 0.014

9.867 ± 0.048

8.152 ± 0.05

9.566 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.024

5.553 ± 0.033

3.087 ± 0.026

2.836 ± 0.024

3.793 ± 0.024

5.965 ± 0.034

5.075 ± 0.035

6.593 ± 0.04

0.726 ± 0.016

3.989 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski