Shigella dysenteriae serotype 1 (strain Sd197)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Shigella; Shigella dysenteriae

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3897 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q32J74|Q32J74_SHIDS Uncharacterized protein OS=Shigella dysenteriae serotype 1 (strain Sd197) OX=300267 GN=SDY_0422 PE=4 SV=1
MM1 pKa = 7.44GVTAAQVSAWPAGTVNIAVSGEE23 pKa = 4.02SSAGNPISITHH34 pKa = 6.85PVTVDD39 pKa = 3.37LTPAAITINTIATDD53 pKa = 3.79DD54 pKa = 4.18VINAAEE60 pKa = 4.24KK61 pKa = 10.47GANLTLSGTTTNVEE75 pKa = 3.87AGQTVTVTFGGKK87 pKa = 9.87NYY89 pKa = 7.45TASVAGDD96 pKa = 3.94GSWTATVPAADD107 pKa = 4.29LAALPEE113 pKa = 4.73GSASAQASVSNINGNSASAVHH134 pKa = 6.47NYY136 pKa = 10.21SVDD139 pKa = 3.25SSAPTIIINTVASDD153 pKa = 4.03NIVNASEE160 pKa = 4.07ADD162 pKa = 3.3AGVTVSGSTTAEE174 pKa = 3.75AGQIVTITLNSPTVQTYY191 pKa = 8.96QATVQADD198 pKa = 4.12GSWSINIPAADD209 pKa = 4.62LEE211 pKa = 4.22ALTDD215 pKa = 4.13GSHH218 pKa = 6.42TLTATVNDD226 pKa = 4.06KK227 pKa = 11.14AGNPASTTHH236 pKa = 6.32NLAVDD241 pKa = 3.83LTVPVLTINTIAGDD255 pKa = 4.23DD256 pKa = 3.92IINATEE262 pKa = 4.03HH263 pKa = 5.67GQALVISGSSTGGEE277 pKa = 3.94AGDD280 pKa = 3.77VVSVTLNSKK289 pKa = 9.05TYY291 pKa = 7.54TTTLDD296 pKa = 3.47ASGNWSVGVPAADD309 pKa = 3.56VTALGSGPQTVTATVTDD326 pKa = 3.67AAGNSDD332 pKa = 4.11NEE334 pKa = 4.52THH336 pKa = 5.96TVTVNLTAPTIGINTIATDD355 pKa = 3.7DD356 pKa = 4.25VINATEE362 pKa = 4.42KK363 pKa = 11.1GADD366 pKa = 3.7LQISGTSNQPAGTTITVTLNGQNYY390 pKa = 7.45TATTDD395 pKa = 3.95ASGNWSTTVPASAVGALGEE414 pKa = 3.99ASYY417 pKa = 9.98TVTANVTDD425 pKa = 3.6SAGNSNSASHH435 pKa = 6.17NVQVNTALPGVTLNPVASDD454 pKa = 4.36DD455 pKa = 4.26IINAAEE461 pKa = 4.13SGVAQTISGQVTGAAAGDD479 pKa = 4.13TVTVTLGGKK488 pKa = 8.14TYY490 pKa = 9.31TATVAGWW497 pKa = 3.03

Molecular weight: 48.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q329J2|Q329J2_SHIDS Uncharacterized protein OS=Shigella dysenteriae serotype 1 (strain Sd197) OX=300267 GN=yjdB PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3897

0

3897

1094416

14

1588

280.8

31.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.419 ± 0.042

1.191 ± 0.016

5.098 ± 0.033

5.846 ± 0.04

3.796 ± 0.028

7.23 ± 0.043

2.321 ± 0.018

5.86 ± 0.032

4.476 ± 0.035

10.701 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.869 ± 0.02

3.883 ± 0.026

4.354 ± 0.023

4.433 ± 0.033

5.884 ± 0.039

5.815 ± 0.033

5.451 ± 0.035

7.022 ± 0.034

1.516 ± 0.018

2.836 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski