Sporolactobacillus inulinus
Average proteome isoelectric point is 7.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4718 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y1ZG93|A0A4Y1ZG93_9BACL Putative transcriptional regulator OS=Sporolactobacillus inulinus OX=2078 GN=NBRC111894_3535 PE=4 SV=1
MM1 pKa = 7.4 NKK3 pKa = 9.33 QVLYY7 pKa = 10.98 YY8 pKa = 10.85 NIDD11 pKa = 3.54 DD12 pKa = 3.86 SLDD15 pKa = 3.51 YY16 pKa = 10.77 EE17 pKa = 4.27 RR18 pKa = 11.84 QLLTEE23 pKa = 4.26 WKK25 pKa = 10.36 INDD28 pKa = 3.77 LEE30 pKa = 4.63 LIEE33 pKa = 4.38 VKK35 pKa = 10.44 DD36 pKa = 3.78 YY37 pKa = 11.34 EE38 pKa = 4.35 NRR40 pKa = 11.84 NSFVDD45 pKa = 3.69 YY46 pKa = 10.95 AQDD49 pKa = 3.28 ADD51 pKa = 4.08 GVVVEE56 pKa = 4.4 YY57 pKa = 10.47 QQITEE62 pKa = 5.16 DD63 pKa = 4.16 ILNQLPII70 pKa = 4.79
Molecular weight: 8.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.554
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.872
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.796
Protein with the highest isoelectric point:
>tr|A0A4Y1ZDR2|A0A4Y1ZDR2_9BACL Seryl-tRNA synthetase OS=Sporolactobacillus inulinus OX=2078 GN=NBRC111894_2806 PE=4 SV=1
MM1 pKa = 7.25 YY2 pKa = 9.4 PLKK5 pKa = 10.32 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 KK14 pKa = 9.23 RR15 pKa = 11.84 KK16 pKa = 8.22 KK17 pKa = 8.69 VHH19 pKa = 5.45 GFRR22 pKa = 11.84 ARR24 pKa = 11.84 MATANGRR31 pKa = 11.84 KK32 pKa = 9.03 VLAARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 8.81 GRR42 pKa = 11.84 KK43 pKa = 8.75 VLSAA47 pKa = 4.05
Molecular weight: 5.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4718
0
4718
848944
37
1229
179.9
20.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.076 ± 0.042
1.051 ± 0.016
5.144 ± 0.036
5.954 ± 0.043
4.43 ± 0.033
6.719 ± 0.039
2.451 ± 0.021
7.056 ± 0.042
6.392 ± 0.046
10.068 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.961 ± 0.021
3.977 ± 0.03
3.823 ± 0.023
4.081 ± 0.029
5.231 ± 0.035
6.288 ± 0.032
5.307 ± 0.024
6.752 ± 0.029
1.028 ± 0.015
3.212 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here