Amino acid dipepetide frequency for Sporolactobacillus inulinus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.07AlaAla: 8.07 ± 0.144
0.952AlaCys: 0.952 ± 0.03
4.188AlaAsp: 4.188 ± 0.07
4.879AlaGlu: 4.879 ± 0.08
3.685AlaPhe: 3.685 ± 0.07
5.599AlaGly: 5.599 ± 0.094
1.781AlaHis: 1.781 ± 0.048
5.785AlaIle: 5.785 ± 0.086
4.892AlaLys: 4.892 ± 0.075
8.77AlaLeu: 8.77 ± 0.122
2.383AlaMet: 2.383 ± 0.058
2.712AlaAsn: 2.712 ± 0.055
2.48AlaPro: 2.48 ± 0.06
3.05AlaGln: 3.05 ± 0.058
3.738AlaArg: 3.738 ± 0.069
4.944AlaSer: 4.944 ± 0.09
3.551AlaThr: 3.551 ± 0.067
6.046AlaVal: 6.046 ± 0.091
0.709AlaTrp: 0.709 ± 0.028
2.548AlaTyr: 2.548 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.878CysAla: 0.878 ± 0.035
0.23CysCys: 0.23 ± 0.017
0.509CysAsp: 0.509 ± 0.025
0.463CysGlu: 0.463 ± 0.024
0.597CysPhe: 0.597 ± 0.026
0.931CysGly: 0.931 ± 0.035
0.251CysHis: 0.251 ± 0.016
0.605CysIle: 0.605 ± 0.024
0.412CysLys: 0.412 ± 0.022
1.111CysLeu: 1.111 ± 0.039
0.315CysMet: 0.315 ± 0.017
0.307CysAsn: 0.307 ± 0.019
0.489CysPro: 0.489 ± 0.027
0.26CysGln: 0.26 ± 0.016
0.715CysArg: 0.715 ± 0.029
0.846CysSer: 0.846 ± 0.034
0.564CysThr: 0.564 ± 0.023
0.572CysVal: 0.572 ± 0.027
0.11CysTrp: 0.11 ± 0.013
0.345CysTyr: 0.345 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
4.148AspAla: 4.148 ± 0.071
0.508AspCys: 0.508 ± 0.029
2.591AspAsp: 2.591 ± 0.06
3.779AspGlu: 3.779 ± 0.072
2.279AspPhe: 2.279 ± 0.05
3.331AspGly: 3.331 ± 0.069
1.621AspHis: 1.621 ± 0.048
2.798AspIle: 2.798 ± 0.065
2.639AspLys: 2.639 ± 0.055
5.288AspLeu: 5.288 ± 0.073
1.231AspMet: 1.231 ± 0.041
1.438AspAsn: 1.438 ± 0.052
2.324AspPro: 2.324 ± 0.043
2.934AspGln: 2.934 ± 0.065
2.99AspArg: 2.99 ± 0.055
2.681AspSer: 2.681 ± 0.061
2.376AspThr: 2.376 ± 0.051
3.641AspVal: 3.641 ± 0.065
0.61AspTrp: 0.61 ± 0.025
2.23AspTyr: 2.23 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
5.156GluAla: 5.156 ± 0.085
0.43GluCys: 0.43 ± 0.025
2.753GluAsp: 2.753 ± 0.067
4.078GluGlu: 4.078 ± 0.076
1.677GluPhe: 1.677 ± 0.045
3.377GluGly: 3.377 ± 0.066
1.621GluHis: 1.621 ± 0.042
3.784GluIle: 3.784 ± 0.065
5.239GluLys: 5.239 ± 0.087
5.923GluLeu: 5.923 ± 0.1
1.851GluMet: 1.851 ± 0.046
2.697GluAsn: 2.697 ± 0.062
1.821GluPro: 1.821 ± 0.042
3.282GluGln: 3.282 ± 0.073
3.769GluArg: 3.769 ± 0.071
3.137GluSer: 3.137 ± 0.064
3.395GluThr: 3.395 ± 0.056
3.49GluVal: 3.49 ± 0.06
0.686GluTrp: 0.686 ± 0.026
1.373GluTyr: 1.373 ± 0.041
0.0GluXaa: 0.0 ± 0.0
Phe
3.337PheAla: 3.337 ± 0.071
0.541PheCys: 0.541 ± 0.028
2.442PheAsp: 2.442 ± 0.047
2.205PheGlu: 2.205 ± 0.053
2.306PhePhe: 2.306 ± 0.059
3.304PheGly: 3.304 ± 0.071
1.14PheHis: 1.14 ± 0.038
3.318PheIle: 3.318 ± 0.073
2.203PheLys: 2.203 ± 0.055
4.601PheLeu: 4.601 ± 0.099
1.219PheMet: 1.219 ± 0.043
1.702PheAsn: 1.702 ± 0.043
1.646PhePro: 1.646 ± 0.047
1.575PheGln: 1.575 ± 0.043
1.853PheArg: 1.853 ± 0.049
3.435PheSer: 3.435 ± 0.072
2.423PheThr: 2.423 ± 0.055
2.975PheVal: 2.975 ± 0.066
0.464PheTrp: 0.464 ± 0.023
1.609PheTyr: 1.609 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
5.242GlyAla: 5.242 ± 0.099
0.8GlyCys: 0.8 ± 0.033
3.085GlyAsp: 3.085 ± 0.067
3.528GlyGlu: 3.528 ± 0.066
3.312GlyPhe: 3.312 ± 0.071
4.603GlyGly: 4.603 ± 0.092
1.474GlyHis: 1.474 ± 0.048
5.422GlyIle: 5.422 ± 0.081
4.831GlyLys: 4.831 ± 0.08
6.646GlyLeu: 6.646 ± 0.097
2.26GlyMet: 2.26 ± 0.052
2.396GlyAsn: 2.396 ± 0.053
1.651GlyPro: 1.651 ± 0.045
2.346GlyGln: 2.346 ± 0.053
3.276GlyArg: 3.276 ± 0.074
4.278GlySer: 4.278 ± 0.079
3.972GlyThr: 3.972 ± 0.072
4.7GlyVal: 4.7 ± 0.071
0.782GlyTrp: 0.782 ± 0.036
2.581GlyTyr: 2.581 ± 0.057
0.0GlyXaa: 0.0 ± 0.0
His
1.938HisAla: 1.938 ± 0.049
0.336HisCys: 0.336 ± 0.021
1.186HisAsp: 1.186 ± 0.034
1.529HisGlu: 1.529 ± 0.044
1.337HisPhe: 1.337 ± 0.043
1.627HisGly: 1.627 ± 0.041
0.852HisHis: 0.852 ± 0.034
1.336HisIle: 1.336 ± 0.041
1.047HisLys: 1.047 ± 0.034
2.648HisLeu: 2.648 ± 0.051
0.675HisMet: 0.675 ± 0.029
0.707HisAsn: 0.707 ± 0.024
1.378HisPro: 1.378 ± 0.041
1.11HisGln: 1.11 ± 0.038
1.264HisArg: 1.264 ± 0.035
1.376HisSer: 1.376 ± 0.041
1.057HisThr: 1.057 ± 0.035
1.774HisVal: 1.774 ± 0.052
0.302HisTrp: 0.302 ± 0.017
1.031HisTyr: 1.031 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
5.966IleAla: 5.966 ± 0.081
0.815IleCys: 0.815 ± 0.034
4.396IleAsp: 4.396 ± 0.075
4.245IleGlu: 4.245 ± 0.064
2.792IlePhe: 2.792 ± 0.072
5.711IleGly: 5.711 ± 0.085
1.832IleHis: 1.832 ± 0.052
4.742IleIle: 4.742 ± 0.089
4.124IleLys: 4.124 ± 0.079
6.142IleLeu: 6.142 ± 0.102
1.684IleMet: 1.684 ± 0.045
3.08IleAsn: 3.08 ± 0.062
3.018IlePro: 3.018 ± 0.061
2.597IleGln: 2.597 ± 0.057
3.761IleArg: 3.761 ± 0.072
4.41IleSer: 4.41 ± 0.072
3.47IleThr: 3.47 ± 0.063
4.947IleVal: 4.947 ± 0.09
0.656IleTrp: 0.656 ± 0.025
2.167IleTyr: 2.167 ± 0.05
0.0IleXaa: 0.0 ± 0.0
Lys
4.95LysAla: 4.95 ± 0.075
0.389LysCys: 0.389 ± 0.022
3.183LysAsp: 3.183 ± 0.066
4.55LysGlu: 4.55 ± 0.082
1.549LysPhe: 1.549 ± 0.042
3.897LysGly: 3.897 ± 0.074
1.422LysHis: 1.422 ± 0.044
4.16LysIle: 4.16 ± 0.073
5.704LysLys: 5.704 ± 0.084
5.434LysLeu: 5.434 ± 0.079
2.197LysMet: 2.197 ± 0.056
3.02LysAsn: 3.02 ± 0.064
2.339LysPro: 2.339 ± 0.057
3.25LysGln: 3.25 ± 0.065
4.219LysArg: 4.219 ± 0.071
3.604LysSer: 3.604 ± 0.067
3.647LysThr: 3.647 ± 0.06
4.013LysVal: 4.013 ± 0.078
0.687LysTrp: 0.687 ± 0.032
1.707LysTyr: 1.707 ± 0.044
0.0LysXaa: 0.0 ± 0.0
Leu
8.016LeuAla: 8.016 ± 0.105
1.047LeuCys: 1.047 ± 0.034
5.043LeuAsp: 5.043 ± 0.086
5.159LeuGlu: 5.159 ± 0.089
5.272LeuPhe: 5.272 ± 0.094
6.189LeuGly: 6.189 ± 0.095
2.302LeuHis: 2.302 ± 0.052
7.897LeuIle: 7.897 ± 0.103
6.465LeuLys: 6.465 ± 0.096
10.351LeuLeu: 10.351 ± 0.147
2.97LeuMet: 2.97 ± 0.063
4.537LeuAsn: 4.537 ± 0.073
4.274LeuPro: 4.274 ± 0.078
3.395LeuGln: 3.395 ± 0.059
4.752LeuArg: 4.752 ± 0.078
7.387LeuSer: 7.387 ± 0.109
5.678LeuThr: 5.678 ± 0.08
6.197LeuVal: 6.197 ± 0.082
0.919LeuTrp: 0.919 ± 0.032
2.834LeuTyr: 2.834 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
2.264MetAla: 2.264 ± 0.054
0.198MetCys: 0.198 ± 0.017
1.604MetAsp: 1.604 ± 0.044
1.561MetGlu: 1.561 ± 0.039
0.953MetPhe: 0.953 ± 0.035
1.742MetGly: 1.742 ± 0.045
0.637MetHis: 0.637 ± 0.028
2.661MetIle: 2.661 ± 0.055
2.306MetLys: 2.306 ± 0.045
2.748MetLeu: 2.748 ± 0.063
0.992MetMet: 0.992 ± 0.037
1.681MetAsn: 1.681 ± 0.042
1.249MetPro: 1.249 ± 0.038
1.059MetGln: 1.059 ± 0.04
1.643MetArg: 1.643 ± 0.046
1.749MetSer: 1.749 ± 0.043
1.911MetThr: 1.911 ± 0.05
1.82MetVal: 1.82 ± 0.045
0.167MetTrp: 0.167 ± 0.014
0.666MetTyr: 0.666 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.069AsnAla: 3.069 ± 0.06
0.415AsnCys: 0.415 ± 0.022
2.194AsnAsp: 2.194 ± 0.051
2.554AsnGlu: 2.554 ± 0.058
1.491AsnPhe: 1.491 ± 0.048
3.129AsnGly: 3.129 ± 0.065
1.243AsnHis: 1.243 ± 0.034
2.244AsnIle: 2.244 ± 0.05
2.483AsnLys: 2.483 ± 0.057
3.451AsnLeu: 3.451 ± 0.065
1.066AsnMet: 1.066 ± 0.037
1.485AsnAsn: 1.485 ± 0.043
1.988AsnPro: 1.988 ± 0.041
2.13AsnGln: 2.13 ± 0.055
2.408AsnArg: 2.408 ± 0.054
1.938AsnSer: 1.938 ± 0.058
1.884AsnThr: 1.884 ± 0.047
2.597AsnVal: 2.597 ± 0.061
0.518AsnTrp: 0.518 ± 0.025
1.484AsnTyr: 1.484 ± 0.049
0.0AsnXaa: 0.0 ± 0.0
Pro
2.787ProAla: 2.787 ± 0.056
0.343ProCys: 0.343 ± 0.019
2.231ProAsp: 2.231 ± 0.05
2.993ProGlu: 2.993 ± 0.054
2.071ProPhe: 2.071 ± 0.053
2.437ProGly: 2.437 ± 0.052
0.866ProHis: 0.866 ± 0.03
2.877ProIle: 2.877 ± 0.055
2.244ProLys: 2.244 ± 0.055
3.729ProLeu: 3.729 ± 0.066
1.073ProMet: 1.073 ± 0.032
1.654ProAsn: 1.654 ± 0.047
1.053ProPro: 1.053 ± 0.041
1.447ProGln: 1.447 ± 0.042
1.424ProArg: 1.424 ± 0.039
2.48ProSer: 2.48 ± 0.059
1.875ProThr: 1.875 ± 0.041
2.933ProVal: 2.933 ± 0.06
0.375ProTrp: 0.375 ± 0.023
1.337ProTyr: 1.337 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
3.628GlnAla: 3.628 ± 0.073
0.312GlnCys: 0.312 ± 0.018
1.642GlnAsp: 1.642 ± 0.046
2.35GlnGlu: 2.35 ± 0.053
1.617GlnPhe: 1.617 ± 0.047
2.146GlnGly: 2.146 ± 0.057
0.914GlnHis: 0.914 ± 0.029
2.869GlnIle: 2.869 ± 0.063
2.951GlnLys: 2.951 ± 0.054
4.557GlnLeu: 4.557 ± 0.085
1.391GlnMet: 1.391 ± 0.047
1.768GlnAsn: 1.768 ± 0.046
1.604GlnPro: 1.604 ± 0.047
1.948GlnGln: 1.948 ± 0.051
2.058GlnArg: 2.058 ± 0.048
2.584GlnSer: 2.584 ± 0.054
2.432GlnThr: 2.432 ± 0.062
2.558GlnVal: 2.558 ± 0.055
0.478GlnTrp: 0.478 ± 0.026
1.0GlnTyr: 1.0 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
3.702ArgAla: 3.702 ± 0.065
0.621ArgCys: 0.621 ± 0.03
2.398ArgAsp: 2.398 ± 0.057
3.164ArgGlu: 3.164 ± 0.072
2.483ArgPhe: 2.483 ± 0.057
2.656ArgGly: 2.656 ± 0.055
1.278ArgHis: 1.278 ± 0.038
3.786ArgIle: 3.786 ± 0.075
3.648ArgLys: 3.648 ± 0.07
5.589ArgLeu: 5.589 ± 0.081
1.819ArgMet: 1.819 ± 0.052
2.037ArgAsn: 2.037 ± 0.049
1.854ArgPro: 1.854 ± 0.049
2.262ArgGln: 2.262 ± 0.054
3.219ArgArg: 3.219 ± 0.063
3.537ArgSer: 3.537 ± 0.068
2.785ArgThr: 2.785 ± 0.056
3.133ArgVal: 3.133 ± 0.067
0.509ArgTrp: 0.509 ± 0.021
1.833ArgTyr: 1.833 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
4.934SerAla: 4.934 ± 0.08
0.781SerCys: 0.781 ± 0.033
3.39SerAsp: 3.39 ± 0.062
3.941SerGlu: 3.941 ± 0.073
3.052SerPhe: 3.052 ± 0.067
5.199SerGly: 5.199 ± 0.087
1.304SerHis: 1.304 ± 0.04
4.688SerIle: 4.688 ± 0.08
3.596SerLys: 3.596 ± 0.062
6.297SerLeu: 6.297 ± 0.085
1.977SerMet: 1.977 ± 0.046
2.191SerAsn: 2.191 ± 0.053
2.097SerPro: 2.097 ± 0.048
2.021SerGln: 2.021 ± 0.045
3.041SerArg: 3.041 ± 0.057
4.217SerSer: 4.217 ± 0.092
2.968SerThr: 2.968 ± 0.05
4.559SerVal: 4.559 ± 0.08
0.701SerTrp: 0.701 ± 0.03
1.921SerTyr: 1.921 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
4.363ThrAla: 4.363 ± 0.079
0.547ThrCys: 0.547 ± 0.024
2.789ThrAsp: 2.789 ± 0.061
2.951ThrGlu: 2.951 ± 0.056
2.514ThrPhe: 2.514 ± 0.061
4.069ThrGly: 4.069 ± 0.07
1.244ThrHis: 1.244 ± 0.04
4.069ThrIle: 4.069 ± 0.068
2.951ThrLys: 2.951 ± 0.061
5.573ThrLeu: 5.573 ± 0.074
1.471ThrMet: 1.471 ± 0.046
2.041ThrAsn: 2.041 ± 0.05
2.235ThrPro: 2.235 ± 0.055
1.692ThrGln: 1.692 ± 0.044
2.31ThrArg: 2.31 ± 0.055
3.039ThrSer: 3.039 ± 0.062
2.709ThrThr: 2.709 ± 0.064
4.27ThrVal: 4.27 ± 0.075
0.469ThrTrp: 0.469 ± 0.025
1.77ThrTyr: 1.77 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
5.28ValAla: 5.28 ± 0.081
0.806ValCys: 0.806 ± 0.029
3.643ValAsp: 3.643 ± 0.065
3.29ValGlu: 3.29 ± 0.063
3.039ValPhe: 3.039 ± 0.065
4.304ValGly: 4.304 ± 0.082
1.628ValHis: 1.628 ± 0.046
4.875ValIle: 4.875 ± 0.08
3.926ValLys: 3.926 ± 0.066
7.068ValLeu: 7.068 ± 0.093
1.896ValMet: 1.896 ± 0.05
2.742ValAsn: 2.742 ± 0.059
2.899ValPro: 2.899 ± 0.054
2.536ValGln: 2.536 ± 0.055
3.421ValArg: 3.421 ± 0.067
4.6ValSer: 4.6 ± 0.065
4.137ValThr: 4.137 ± 0.07
4.705ValVal: 4.705 ± 0.079
0.611ValTrp: 0.611 ± 0.028
2.114ValTyr: 2.114 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
0.683TrpAla: 0.683 ± 0.029
0.092TrpCys: 0.092 ± 0.01
0.441TrpAsp: 0.441 ± 0.025
0.446TrpGlu: 0.446 ± 0.024
0.532TrpPhe: 0.532 ± 0.026
0.614TrpGly: 0.614 ± 0.026
0.247TrpHis: 0.247 ± 0.015
0.815TrpIle: 0.815 ± 0.031
0.66TrpLys: 0.66 ± 0.026
1.25TrpLeu: 1.25 ± 0.045
0.36TrpMet: 0.36 ± 0.02
0.471TrpAsn: 0.471 ± 0.024
0.377TrpPro: 0.377 ± 0.021
0.432TrpGln: 0.432 ± 0.021
0.64TrpArg: 0.64 ± 0.027
0.663TrpSer: 0.663 ± 0.028
0.564TrpThr: 0.564 ± 0.026
0.545TrpVal: 0.545 ± 0.027
0.137TrpTrp: 0.137 ± 0.012
0.309TrpTyr: 0.309 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.35TyrAla: 2.35 ± 0.052
0.35TyrCys: 0.35 ± 0.018
1.727TyrAsp: 1.727 ± 0.046
1.873TyrGlu: 1.873 ± 0.049
1.739TyrPhe: 1.739 ± 0.043
2.322TyrGly: 2.322 ± 0.051
0.852TyrHis: 0.852 ± 0.027
1.826TyrIle: 1.826 ± 0.044
1.602TyrLys: 1.602 ± 0.046
3.507TyrLeu: 3.507 ± 0.061
0.78TyrMet: 0.78 ± 0.029
1.103TyrAsn: 1.103 ± 0.039
1.452TyrPro: 1.452 ± 0.043
1.485TyrGln: 1.485 ± 0.04
1.813TyrArg: 1.813 ± 0.049
1.972TyrSer: 1.972 ± 0.052
1.676TyrThr: 1.676 ± 0.047
2.043TyrVal: 2.043 ± 0.044
0.39TyrTrp: 0.39 ± 0.02
1.263TyrTyr: 1.263 ± 0.046
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4718 proteins (848944 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski