Tortoise microvirus 67

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W6L0|A0A4P8W6L0_9VIRU Replication initiation protein OS=Tortoise microvirus 67 OX=2583173 PE=4 SV=1
MM1 pKa = 7.37LRR3 pKa = 11.84YY4 pKa = 10.12DD5 pKa = 5.01GISSLGGCNLVEE17 pKa = 4.14YY18 pKa = 10.31DD19 pKa = 4.72PPYY22 pKa = 10.93LDD24 pKa = 4.63ADD26 pKa = 4.11FGEE29 pKa = 4.79SVTDD33 pKa = 3.65DD34 pKa = 3.29SYY36 pKa = 12.02YY37 pKa = 10.26IPTPDD42 pKa = 3.31NVGLKK47 pKa = 10.11SIPLTSSEE55 pKa = 4.15ILSNFDD61 pKa = 3.39FSDD64 pKa = 3.63GRR66 pKa = 11.84DD67 pKa = 3.18TGAPVPKK74 pKa = 9.73FRR76 pKa = 11.84KK77 pKa = 9.35PGADD81 pKa = 3.05LGEE84 pKa = 4.41VSQHH88 pKa = 4.87LRR90 pKa = 11.84EE91 pKa = 4.14VQGEE95 pKa = 4.27IKK97 pKa = 10.7SEE99 pKa = 4.2VARR102 pKa = 11.84KK103 pKa = 8.95AAEE106 pKa = 3.71KK107 pKa = 10.78ALADD111 pKa = 3.9KK112 pKa = 11.28YY113 pKa = 11.12KK114 pKa = 10.16VTDD117 pKa = 3.94SPDD120 pKa = 3.49TPHH123 pKa = 6.96APSVAASPNSVV134 pKa = 2.73

Molecular weight: 14.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W6Y3|A0A4P8W6Y3_9VIRU Uncharacterized protein OS=Tortoise microvirus 67 OX=2583173 PE=4 SV=1
MM1 pKa = 7.45SCLFPVTVYY10 pKa = 10.92PNGKK14 pKa = 9.05KK15 pKa = 10.23APPVEE20 pKa = 4.45APCRR24 pKa = 11.84HH25 pKa = 6.31CANCRR30 pKa = 11.84MAKK33 pKa = 10.1QITLNFAIGHH43 pKa = 7.04ALQDD47 pKa = 3.86CYY49 pKa = 11.72SRR51 pKa = 11.84GGSASFVTMTYY62 pKa = 11.08ADD64 pKa = 3.75QFLPDD69 pKa = 3.77NGSLRR74 pKa = 11.84SKK76 pKa = 11.28DD77 pKa = 3.16FTNWLKK83 pKa = 10.77GARR86 pKa = 11.84TKK88 pKa = 10.88LFRR91 pKa = 11.84RR92 pKa = 11.84KK93 pKa = 9.11IDD95 pKa = 3.54LPFKK99 pKa = 10.0YY100 pKa = 9.74LAAGEE105 pKa = 4.21YY106 pKa = 10.0GGKK109 pKa = 9.95LGRR112 pKa = 11.84PHH114 pKa = 5.56YY115 pKa = 10.23HH116 pKa = 7.2CILIGIPRR124 pKa = 11.84PVAEE128 pKa = 4.51GVFRR132 pKa = 11.84PLWRR136 pKa = 11.84HH137 pKa = 5.05GHH139 pKa = 5.69CQTEE143 pKa = 4.35VLGSGGVRR151 pKa = 11.84YY152 pKa = 10.08VSEE155 pKa = 4.62YY156 pKa = 9.89ISKK159 pKa = 10.71QIGGRR164 pKa = 11.84LAEE167 pKa = 3.95QMYY170 pKa = 9.79DD171 pKa = 3.11AKK173 pKa = 10.98GLEE176 pKa = 4.14RR177 pKa = 11.84PFMRR181 pKa = 11.84RR182 pKa = 11.84SVNFCDD188 pKa = 4.78DD189 pKa = 4.64WILKK193 pKa = 10.21NYY195 pKa = 10.07ADD197 pKa = 4.2LVSNGWLFKK206 pKa = 10.91SRR208 pKa = 11.84GKK210 pKa = 9.21MRR212 pKa = 11.84PVPSYY217 pKa = 10.53YY218 pKa = 7.95VHH220 pKa = 7.37KK221 pKa = 10.93YY222 pKa = 10.2FGDD225 pKa = 3.55VDD227 pKa = 3.96LHH229 pKa = 6.73FLAQEE234 pKa = 3.93RR235 pKa = 11.84NLAAKK240 pKa = 8.94RR241 pKa = 11.84AKK243 pKa = 9.72MSLTDD248 pKa = 3.46YY249 pKa = 11.25EE250 pKa = 4.49FMRR253 pKa = 11.84NYY255 pKa = 9.2TLEE258 pKa = 4.34GMSHH262 pKa = 6.4RR263 pKa = 11.84ASRR266 pKa = 11.84AKK268 pKa = 10.46GVASDD273 pKa = 3.58SSSYY277 pKa = 11.67DD278 pKa = 3.02LMPFHH283 pKa = 7.26SSCDD287 pKa = 3.19IASLVKK293 pKa = 10.59DD294 pKa = 4.02ALDD297 pKa = 3.59PVPFF301 pKa = 4.8

Molecular weight: 34.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1670

77

614

238.6

26.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.323 ± 0.682

1.557 ± 0.615

5.868 ± 0.453

4.97 ± 0.66

5.928 ± 0.786

6.886 ± 0.485

1.617 ± 0.376

4.85 ± 0.493

3.832 ± 0.833

8.263 ± 0.585

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.335 ± 0.273

4.311 ± 0.462

5.09 ± 0.797

4.012 ± 1.493

6.048 ± 0.631

9.281 ± 0.689

5.389 ± 0.974

5.689 ± 0.632

0.719 ± 0.178

5.03 ± 0.404
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski