Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans R2)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; Synechococcus elongatus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2659 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q31LJ7|Q31LJ7_SYNE7 Uncharacterized protein OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=Synpcc7942_2042 PE=4 SV=1
MM1 pKa = 7.6ALIYY5 pKa = 9.82PDD7 pKa = 3.31NLRR10 pKa = 11.84YY11 pKa = 9.27FDD13 pKa = 3.24SHH15 pKa = 6.64EE16 pKa = 3.95YY17 pKa = 10.38VRR19 pKa = 11.84LDD21 pKa = 3.07GDD23 pKa = 3.52IAVIGISAYY32 pKa = 10.42AIDD35 pKa = 3.93QLGDD39 pKa = 3.39IVFLEE44 pKa = 4.3LPEE47 pKa = 4.35VGSTIAIGASFGTVEE62 pKa = 4.11SVKK65 pKa = 10.69AVEE68 pKa = 4.08EE69 pKa = 4.63VYY71 pKa = 11.04APVTGEE77 pKa = 3.48IIEE80 pKa = 4.32RR81 pKa = 11.84NEE83 pKa = 3.84AALEE87 pKa = 4.09APEE90 pKa = 4.67ILNSDD95 pKa = 4.12PYY97 pKa = 9.62EE98 pKa = 4.11QGWLLKK104 pKa = 10.53VQLTGKK110 pKa = 9.5PDD112 pKa = 3.4LSDD115 pKa = 3.99SYY117 pKa = 11.77DD118 pKa = 3.38AAQYY122 pKa = 9.18QALVEE127 pKa = 4.39GQQ129 pKa = 3.12

Molecular weight: 14.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q31MT7|Q31MT7_SYNE7 tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=trmJ PE=3 SV=1
MM1 pKa = 7.56TKK3 pKa = 9.09RR4 pKa = 11.84TLEE7 pKa = 3.76GTNRR11 pKa = 11.84KK12 pKa = 9.13RR13 pKa = 11.84KK14 pKa = 7.54RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84SATGRR29 pKa = 11.84RR30 pKa = 11.84VIKK33 pKa = 10.33ARR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.64GRR40 pKa = 11.84ARR42 pKa = 11.84LAVV45 pKa = 3.42

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2657

2

2659

809334

29

1807

304.4

33.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.545 ± 0.06

1.082 ± 0.019

5.137 ± 0.033

5.706 ± 0.05

3.465 ± 0.031

7.195 ± 0.047

1.724 ± 0.025

5.442 ± 0.036

2.588 ± 0.034

12.312 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.563 ± 0.02

2.749 ± 0.033

5.492 ± 0.037

6.144 ± 0.052

6.796 ± 0.039

6.022 ± 0.036

5.209 ± 0.034

6.629 ± 0.039

1.749 ± 0.032

2.454 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski