Hemidesmus yellow mosaic virus
Average proteome isoelectric point is 8.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S5VP26|S5VP26_9GEMI AC4 OS=Hemidesmus yellow mosaic virus OX=1383052 PE=3 SV=1
MM1 pKa = 7.35 PRR3 pKa = 11.84 SGRR6 pKa = 11.84 FSINAKK12 pKa = 10.33 NYY14 pKa = 7.78 FLTYY18 pKa = 9.37 PRR20 pKa = 11.84 CSLTKK25 pKa = 10.57 EE26 pKa = 3.82 EE27 pKa = 4.73 TIEE30 pKa = 3.87 QLRR33 pKa = 11.84 NLQYY37 pKa = 8.35 PTNLKK42 pKa = 9.42 YY43 pKa = 10.67 IKK45 pKa = 10.07 VCRR48 pKa = 11.84 EE49 pKa = 3.48 LHH51 pKa = 6.31 EE52 pKa = 4.82 NGEE55 pKa = 4.25 PHH57 pKa = 6.94 LHH59 pKa = 6.18 VLLQFEE65 pKa = 4.74 GKK67 pKa = 10.02 YY68 pKa = 10.61 NCTSDD73 pKa = 3.82 RR74 pKa = 11.84 FFDD77 pKa = 4.35 LSHH80 pKa = 7.16 PSCSASFHH88 pKa = 6.66 PNIQRR93 pKa = 11.84 AKK95 pKa = 10.0 SSSDD99 pKa = 2.93 VKK101 pKa = 11.11 AYY103 pKa = 9.65 IGKK106 pKa = 10.08 DD107 pKa = 3.06 GDD109 pKa = 3.83 TLDD112 pKa = 3.29 WGEE115 pKa = 4.11 FQVDD119 pKa = 3.59 DD120 pKa = 5.3 EE121 pKa = 4.49 ICKK124 pKa = 10.08 RR125 pKa = 11.84 RR126 pKa = 11.84 PEE128 pKa = 3.65 ICKK131 pKa = 10.07 RR132 pKa = 11.84 CIRR135 pKa = 11.84 LSNNAGSKK143 pKa = 10.16 QEE145 pKa = 3.85 ALNIIKK151 pKa = 10.25 EE152 pKa = 4.38 LMPKK156 pKa = 10.28 DD157 pKa = 3.82 YY158 pKa = 11.11 VLQYY162 pKa = 11.27 HH163 pKa = 6.06 NLHH166 pKa = 6.67 SNFDD170 pKa = 4.4 RR171 pKa = 11.84 IFTSAIPPYY180 pKa = 9.82 KK181 pKa = 10.58 SPFLSSSFDD190 pKa = 3.47 QVPDD194 pKa = 3.66 EE195 pKa = 4.24 LEE197 pKa = 3.85 EE198 pKa = 4.2 WVSEE202 pKa = 4.1 NVLPATARR210 pKa = 11.84 PEE212 pKa = 3.96 RR213 pKa = 11.84 PISIVVEE220 pKa = 4.09 GDD222 pKa = 2.99 SRR224 pKa = 11.84 TGKK227 pKa = 7.64 TLWARR232 pKa = 11.84 SLGPHH237 pKa = 6.71 NYY239 pKa = 10.18 LCGHH243 pKa = 7.38 LDD245 pKa = 3.63 LSPRR249 pKa = 11.84 VFSNDD254 pKa = 1.85 AWYY257 pKa = 10.93 NVIDD261 pKa = 5.2 DD262 pKa = 4.41 VDD264 pKa = 3.61 PHH266 pKa = 5.85 YY267 pKa = 11.0 LKK269 pKa = 10.7 HH270 pKa = 6.2 FKK272 pKa = 10.7 EE273 pKa = 4.46 FMGAQRR279 pKa = 11.84 DD280 pKa = 3.81 WQSNTKK286 pKa = 9.2 YY287 pKa = 10.5 GKK289 pKa = 9.24 PIQVRR294 pKa = 11.84 GGIPTIFLCNPGPTSSYY311 pKa = 11.11 TEE313 pKa = 3.94 FLDD316 pKa = 3.76 EE317 pKa = 4.95 EE318 pKa = 4.66 KK319 pKa = 11.09 NKK321 pKa = 10.49 SLKK324 pKa = 10.33 AWAIKK329 pKa = 9.32 NARR332 pKa = 11.84 FITLFQPLFSGTNQSPTQPGQEE354 pKa = 3.57 EE355 pKa = 4.59 DD356 pKa = 4.19 LPQEE360 pKa = 4.37 EE361 pKa = 4.46 NN362 pKa = 3.23
Molecular weight: 41.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.912
IPC2_protein 5.919
IPC_protein 5.982
Toseland 6.3
ProMoST 6.351
Dawson 6.287
Bjellqvist 6.275
Wikipedia 6.3
Rodwell 6.275
Grimsley 6.427
Solomon 6.287
Lehninger 6.287
Nozaki 6.561
DTASelect 6.737
Thurlkill 6.766
EMBOSS 6.737
Sillero 6.664
Patrickios 4.088
IPC_peptide 6.3
IPC2_peptide 6.605
IPC2.peptide.svr19 6.586
Protein with the highest isoelectric point:
>tr|S5WAB7|S5WAB7_9GEMI Capsid protein OS=Hemidesmus yellow mosaic virus OX=1383052 PE=3 SV=1
MM1 pKa = 7.55 SKK3 pKa = 10.09 RR4 pKa = 11.84 VADD7 pKa = 3.34 IGVFTPASKK16 pKa = 10.36 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.12 SPYY27 pKa = 10.07 KK28 pKa = 10.54 SRR30 pKa = 11.84 AAVPIVPVTRR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 SWVYY47 pKa = 9.19 RR48 pKa = 11.84 PSYY51 pKa = 9.36 RR52 pKa = 11.84 KK53 pKa = 8.26 PWMYY57 pKa = 11.31 RR58 pKa = 11.84 MFRR61 pKa = 11.84 SPDD64 pKa = 3.23 VPRR67 pKa = 11.84 GCEE70 pKa = 4.34 GPCKK74 pKa = 10.21 VQSFEE79 pKa = 3.84 QRR81 pKa = 11.84 HH82 pKa = 6.27 DD83 pKa = 3.18 IAHH86 pKa = 6.13 TGKK89 pKa = 10.45 VICISDD95 pKa = 3.52 VTRR98 pKa = 11.84 GGGITHH104 pKa = 7.09 RR105 pKa = 11.84 VGKK108 pKa = 9.44 RR109 pKa = 11.84 FCVKK113 pKa = 9.73 SVYY116 pKa = 10.19 IIGKK120 pKa = 8.51 IWMDD124 pKa = 3.34 KK125 pKa = 10.05 NIKK128 pKa = 8.98 VKK130 pKa = 10.57 NHH132 pKa = 5.62 TNSVMFWLVRR142 pKa = 11.84 DD143 pKa = 3.83 RR144 pKa = 11.84 RR145 pKa = 11.84 PFGSPMDD152 pKa = 4.16 FGQVFNMYY160 pKa = 10.66 DD161 pKa = 3.86 NEE163 pKa = 4.34 PSTATVKK170 pKa = 10.81 NDD172 pKa = 2.84 LRR174 pKa = 11.84 DD175 pKa = 3.6 RR176 pKa = 11.84 YY177 pKa = 9.88 QVLHH181 pKa = 6.71 RR182 pKa = 11.84 FSATVTGGQYY192 pKa = 10.85 ASKK195 pKa = 10.24 EE196 pKa = 3.73 QALVKK201 pKa = 10.56 KK202 pKa = 10.06 FWKK205 pKa = 9.38 INNYY209 pKa = 8.37 VVYY212 pKa = 10.35 NHH214 pKa = 6.03 QEE216 pKa = 3.42 AAKK219 pKa = 10.4 YY220 pKa = 9.25 EE221 pKa = 4.13 NHH223 pKa = 6.46 TEE225 pKa = 3.99 NALLLYY231 pKa = 7.29 MACTHH236 pKa = 7.07 ASNPVYY242 pKa = 9.86 ATLKK246 pKa = 9.47 IRR248 pKa = 11.84 IYY250 pKa = 10.59 FYY252 pKa = 11.32 DD253 pKa = 4.06 SISNN257 pKa = 3.66
Molecular weight: 29.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.286
IPC2_protein 9.648
IPC_protein 9.984
Toseland 10.189
ProMoST 9.926
Dawson 10.409
Bjellqvist 10.101
Wikipedia 10.599
Rodwell 10.745
Grimsley 10.482
Solomon 10.423
Lehninger 10.394
Nozaki 10.204
DTASelect 10.087
Thurlkill 10.248
EMBOSS 10.599
Sillero 10.321
Patrickios 10.292
IPC_peptide 10.423
IPC2_peptide 9.004
IPC2.peptide.svr19 8.579
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1215
98
362
173.6
19.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.938 ± 0.518
2.551 ± 0.302
4.691 ± 0.447
4.856 ± 1.091
4.691 ± 0.626
4.444 ± 0.37
3.868 ± 0.753
5.679 ± 0.702
5.267 ± 0.711
7.407 ± 1.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.728 ± 0.452
4.691 ± 0.624
6.667 ± 0.828
4.856 ± 0.697
7.407 ± 0.807
9.547 ± 1.411
5.267 ± 0.703
6.173 ± 1.206
1.481 ± 0.249
3.786 ± 0.574
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here