Mycobacterium phage BoostSeason

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KDZ0|A0A3G2KDZ0_9CAUD Lysin B OS=Mycobacterium phage BoostSeason OX=2419977 GN=30 PE=4 SV=1
MM1 pKa = 7.64SNPDD5 pKa = 3.68DD6 pKa = 5.93DD7 pKa = 4.49YY8 pKa = 11.28TFCVYY13 pKa = 11.04YY14 pKa = 10.26EE15 pKa = 4.16GQPVPGGPWQPYY27 pKa = 8.08VAVAPSLADD36 pKa = 3.25AEE38 pKa = 4.45QWVEE42 pKa = 3.88LLAAAVDD49 pKa = 3.77GNPYY53 pKa = 9.58VRR55 pKa = 11.84NLTIGYY61 pKa = 8.82APKK64 pKa = 9.62VTWQPWPPGDD74 pKa = 3.49GG75 pKa = 3.34

Molecular weight: 8.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KEA1|A0A3G2KEA1_9CAUD Uncharacterized protein OS=Mycobacterium phage BoostSeason OX=2419977 GN=77 PE=4 SV=1
MM1 pKa = 7.7SDD3 pKa = 3.94LVIFDD8 pKa = 4.71EE9 pKa = 4.6LQQGTGDD16 pKa = 3.66EE17 pKa = 4.2PRR19 pKa = 11.84DD20 pKa = 3.5YY21 pKa = 11.53VPAFEE26 pKa = 4.72RR27 pKa = 11.84AVLYY31 pKa = 11.04AMQFKK36 pKa = 10.19PMYY39 pKa = 9.11EE40 pKa = 3.84GTVPHH45 pKa = 6.92RR46 pKa = 11.84VRR48 pKa = 11.84AKK50 pKa = 8.72RR51 pKa = 11.84RR52 pKa = 11.84EE53 pKa = 3.96RR54 pKa = 11.84NRR56 pKa = 11.84VARR59 pKa = 11.84RR60 pKa = 11.84SRR62 pKa = 11.84KK63 pKa = 9.07INRR66 pKa = 11.84KK67 pKa = 9.51ASS69 pKa = 3.24

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

18157

38

1224

193.2

20.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.102 ± 0.384

1.212 ± 0.153

6.526 ± 0.205

5.596 ± 0.325

2.638 ± 0.163

8.873 ± 0.532

2.418 ± 0.195

3.789 ± 0.176

3.53 ± 0.257

8.399 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.297 ± 0.117

2.677 ± 0.158

5.728 ± 0.225

3.349 ± 0.147

7.083 ± 0.386

5.1 ± 0.279

5.75 ± 0.203

7.688 ± 0.289

2.093 ± 0.118

2.153 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski