Amino acid dipepetide frequency for Mycobacterium phage BoostSeason

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.048AlaAla: 20.048 ± 1.509
1.157AlaCys: 1.157 ± 0.242
8.207AlaAsp: 8.207 ± 0.606
9.694AlaGlu: 9.694 ± 1.074
2.864AlaPhe: 2.864 ± 0.373
8.813AlaGly: 8.813 ± 0.799
3.745AlaHis: 3.745 ± 0.449
5.398AlaIle: 5.398 ± 0.57
4.516AlaLys: 4.516 ± 0.54
12.172AlaLeu: 12.172 ± 0.906
3.911AlaMet: 3.911 ± 0.509
2.809AlaAsn: 2.809 ± 0.438
6.334AlaPro: 6.334 ± 0.544
5.288AlaGln: 5.288 ± 0.542
8.317AlaArg: 8.317 ± 0.654
5.728AlaSer: 5.728 ± 0.608
6.83AlaThr: 6.83 ± 0.793
10.134AlaVal: 10.134 ± 0.974
2.974AlaTrp: 2.974 ± 0.393
2.093AlaTyr: 2.093 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
1.487CysAla: 1.487 ± 0.324
0.22CysCys: 0.22 ± 0.115
0.606CysAsp: 0.606 ± 0.237
0.826CysGlu: 0.826 ± 0.214
0.33CysPhe: 0.33 ± 0.13
1.102CysGly: 1.102 ± 0.212
0.275CysHis: 0.275 ± 0.125
0.386CysIle: 0.386 ± 0.16
0.441CysLys: 0.441 ± 0.192
0.606CysLeu: 0.606 ± 0.178
0.441CysMet: 0.441 ± 0.161
0.275CysAsn: 0.275 ± 0.119
0.936CysPro: 0.936 ± 0.23
0.33CysGln: 0.33 ± 0.171
0.991CysArg: 0.991 ± 0.22
1.157CysSer: 1.157 ± 0.2
0.496CysThr: 0.496 ± 0.179
0.826CysVal: 0.826 ± 0.244
0.165CysTrp: 0.165 ± 0.097
0.22CysTyr: 0.22 ± 0.094
0.0CysXaa: 0.0 ± 0.0
Asp
7.38AspAla: 7.38 ± 0.559
0.936AspCys: 0.936 ± 0.229
5.453AspAsp: 5.453 ± 0.712
5.012AspGlu: 5.012 ± 0.543
1.377AspPhe: 1.377 ± 0.255
5.893AspGly: 5.893 ± 0.557
0.551AspHis: 0.551 ± 0.161
1.652AspIle: 1.652 ± 0.317
2.589AspLys: 2.589 ± 0.39
5.728AspLeu: 5.728 ± 0.554
1.983AspMet: 1.983 ± 0.366
2.093AspAsn: 2.093 ± 0.329
5.343AspPro: 5.343 ± 0.592
1.267AspGln: 1.267 ± 0.244
4.406AspArg: 4.406 ± 0.508
3.305AspSer: 3.305 ± 0.365
3.36AspThr: 3.36 ± 0.354
4.406AspVal: 4.406 ± 0.577
0.771AspTrp: 0.771 ± 0.183
1.763AspTyr: 1.763 ± 0.369
0.0AspXaa: 0.0 ± 0.0
Glu
7.876GluAla: 7.876 ± 0.807
0.991GluCys: 0.991 ± 0.25
2.313GluAsp: 2.313 ± 0.388
1.763GluGlu: 1.763 ± 0.317
1.542GluPhe: 1.542 ± 0.33
4.241GluGly: 4.241 ± 0.525
1.928GluHis: 1.928 ± 0.358
1.818GluIle: 1.818 ± 0.303
1.983GluLys: 1.983 ± 0.484
5.893GluLeu: 5.893 ± 0.492
1.212GluMet: 1.212 ± 0.282
0.991GluAsn: 0.991 ± 0.219
3.69GluPro: 3.69 ± 0.562
2.864GluGln: 2.864 ± 0.431
5.122GluArg: 5.122 ± 0.639
2.479GluSer: 2.479 ± 0.35
1.873GluThr: 1.873 ± 0.307
5.122GluVal: 5.122 ± 0.618
0.826GluTrp: 0.826 ± 0.202
1.432GluTyr: 1.432 ± 0.292
0.0GluXaa: 0.0 ± 0.0
Phe
2.809PheAla: 2.809 ± 0.37
0.33PheCys: 0.33 ± 0.137
2.864PheAsp: 2.864 ± 0.38
1.818PheGlu: 1.818 ± 0.293
0.496PhePhe: 0.496 ± 0.167
2.809PheGly: 2.809 ± 0.415
0.386PheHis: 0.386 ± 0.142
1.046PheIle: 1.046 ± 0.251
1.212PheLys: 1.212 ± 0.305
1.873PheLeu: 1.873 ± 0.4
0.826PheMet: 0.826 ± 0.266
0.661PheAsn: 0.661 ± 0.223
0.606PhePro: 0.606 ± 0.204
0.826PheGln: 0.826 ± 0.176
1.928PheArg: 1.928 ± 0.276
1.102PheSer: 1.102 ± 0.244
1.818PheThr: 1.818 ± 0.302
2.038PheVal: 2.038 ± 0.306
0.33PheTrp: 0.33 ± 0.131
0.551PheTyr: 0.551 ± 0.196
0.0PheXaa: 0.0 ± 0.0
Gly
10.63GlyAla: 10.63 ± 0.769
0.826GlyCys: 0.826 ± 0.213
5.563GlyAsp: 5.563 ± 0.539
4.957GlyGlu: 4.957 ± 0.54
2.313GlyPhe: 2.313 ± 0.361
9.473GlyGly: 9.473 ± 1.22
2.423GlyHis: 2.423 ± 0.372
3.029GlyIle: 3.029 ± 0.55
5.067GlyLys: 5.067 ± 0.608
6.389GlyLeu: 6.389 ± 0.841
2.479GlyMet: 2.479 ± 0.447
3.195GlyAsn: 3.195 ± 0.374
3.69GlyPro: 3.69 ± 0.575
2.589GlyGln: 2.589 ± 0.407
5.673GlyArg: 5.673 ± 0.643
4.682GlySer: 4.682 ± 0.856
5.012GlyThr: 5.012 ± 0.574
6.83GlyVal: 6.83 ± 0.57
2.093GlyTrp: 2.093 ± 0.264
1.818GlyTyr: 1.818 ± 0.289
0.0GlyXaa: 0.0 ± 0.0
His
2.589HisAla: 2.589 ± 0.389
0.386HisCys: 0.386 ± 0.149
1.542HisAsp: 1.542 ± 0.302
0.936HisGlu: 0.936 ± 0.221
0.496HisPhe: 0.496 ± 0.134
1.928HisGly: 1.928 ± 0.359
0.496HisHis: 0.496 ± 0.143
0.991HisIle: 0.991 ± 0.235
0.771HisLys: 0.771 ± 0.204
2.313HisLeu: 2.313 ± 0.389
0.441HisMet: 0.441 ± 0.189
0.936HisAsn: 0.936 ± 0.178
1.707HisPro: 1.707 ± 0.321
0.881HisGln: 0.881 ± 0.248
2.258HisArg: 2.258 ± 0.314
1.046HisSer: 1.046 ± 0.331
1.432HisThr: 1.432 ± 0.276
1.928HisVal: 1.928 ± 0.322
0.551HisTrp: 0.551 ± 0.198
0.551HisTyr: 0.551 ± 0.164
0.0HisXaa: 0.0 ± 0.0
Ile
5.508IleAla: 5.508 ± 0.559
0.165IleCys: 0.165 ± 0.103
3.415IleAsp: 3.415 ± 0.442
2.589IleGlu: 2.589 ± 0.413
0.991IlePhe: 0.991 ± 0.212
3.36IleGly: 3.36 ± 0.487
0.606IleHis: 0.606 ± 0.172
1.046IleIle: 1.046 ± 0.333
1.763IleLys: 1.763 ± 0.441
2.809IleLeu: 2.809 ± 0.445
0.606IleMet: 0.606 ± 0.221
1.763IleAsn: 1.763 ± 0.315
2.313IlePro: 2.313 ± 0.374
0.771IleGln: 0.771 ± 0.191
1.983IleArg: 1.983 ± 0.295
1.873IleSer: 1.873 ± 0.3
2.479IleThr: 2.479 ± 0.324
2.699IleVal: 2.699 ± 0.334
0.496IleTrp: 0.496 ± 0.199
0.661IleTyr: 0.661 ± 0.203
0.0IleXaa: 0.0 ± 0.0
Lys
5.893LysAla: 5.893 ± 0.865
0.606LysCys: 0.606 ± 0.216
1.212LysAsp: 1.212 ± 0.244
0.606LysGlu: 0.606 ± 0.194
1.212LysPhe: 1.212 ± 0.233
3.525LysGly: 3.525 ± 0.666
1.212LysHis: 1.212 ± 0.246
1.377LysIle: 1.377 ± 0.231
0.33LysLys: 0.33 ± 0.12
3.25LysLeu: 3.25 ± 0.376
0.771LysMet: 0.771 ± 0.169
0.551LysAsn: 0.551 ± 0.149
2.148LysPro: 2.148 ± 0.321
0.991LysGln: 0.991 ± 0.24
3.029LysArg: 3.029 ± 0.472
1.928LysSer: 1.928 ± 0.403
2.203LysThr: 2.203 ± 0.34
2.974LysVal: 2.974 ± 0.42
0.826LysTrp: 0.826 ± 0.19
0.661LysTyr: 0.661 ± 0.181
0.0LysXaa: 0.0 ± 0.0
Leu
12.943LeuAla: 12.943 ± 0.807
0.551LeuCys: 0.551 ± 0.182
6.995LeuAsp: 6.995 ± 0.647
2.479LeuGlu: 2.479 ± 0.377
2.093LeuPhe: 2.093 ± 0.374
8.152LeuGly: 8.152 ± 0.68
2.148LeuHis: 2.148 ± 0.345
3.58LeuIle: 3.58 ± 0.431
2.589LeuLys: 2.589 ± 0.367
6.279LeuLeu: 6.279 ± 0.673
1.102LeuMet: 1.102 ± 0.275
2.203LeuAsn: 2.203 ± 0.376
4.847LeuPro: 4.847 ± 0.603
2.479LeuGln: 2.479 ± 0.402
6.059LeuArg: 6.059 ± 0.588
5.122LeuSer: 5.122 ± 0.48
5.177LeuThr: 5.177 ± 0.507
6.059LeuVal: 6.059 ± 0.604
1.652LeuTrp: 1.652 ± 0.248
1.487LeuTyr: 1.487 ± 0.298
0.0LeuXaa: 0.0 ± 0.0
Met
2.644MetAla: 2.644 ± 0.307
0.275MetCys: 0.275 ± 0.126
0.881MetAsp: 0.881 ± 0.207
0.22MetGlu: 0.22 ± 0.13
0.496MetPhe: 0.496 ± 0.181
1.597MetGly: 1.597 ± 0.415
0.496MetHis: 0.496 ± 0.183
0.991MetIle: 0.991 ± 0.188
0.441MetLys: 0.441 ± 0.15
1.542MetLeu: 1.542 ± 0.322
0.441MetMet: 0.441 ± 0.179
0.771MetAsn: 0.771 ± 0.21
1.157MetPro: 1.157 ± 0.244
1.157MetGln: 1.157 ± 0.248
2.093MetArg: 2.093 ± 0.425
2.368MetSer: 2.368 ± 0.394
2.258MetThr: 2.258 ± 0.384
2.093MetVal: 2.093 ± 0.339
0.606MetTrp: 0.606 ± 0.186
0.441MetTyr: 0.441 ± 0.195
0.0MetXaa: 0.0 ± 0.0
Asn
3.415AsnAla: 3.415 ± 0.381
0.386AsnCys: 0.386 ± 0.17
1.212AsnAsp: 1.212 ± 0.278
1.267AsnGlu: 1.267 ± 0.208
0.936AsnPhe: 0.936 ± 0.265
3.525AsnGly: 3.525 ± 0.507
0.551AsnHis: 0.551 ± 0.157
0.826AsnIle: 0.826 ± 0.191
0.716AsnLys: 0.716 ± 0.25
2.038AsnLeu: 2.038 ± 0.343
0.771AsnMet: 0.771 ± 0.189
0.496AsnAsn: 0.496 ± 0.169
2.368AsnPro: 2.368 ± 0.441
0.496AsnGln: 0.496 ± 0.142
1.707AsnArg: 1.707 ± 0.28
1.212AsnSer: 1.212 ± 0.305
1.157AsnThr: 1.157 ± 0.234
2.644AsnVal: 2.644 ± 0.369
0.661AsnTrp: 0.661 ± 0.172
0.386AsnTyr: 0.386 ± 0.154
0.0AsnXaa: 0.0 ± 0.0
Pro
7.05ProAla: 7.05 ± 0.584
0.826ProCys: 0.826 ± 0.309
3.966ProAsp: 3.966 ± 0.465
4.571ProGlu: 4.571 ± 0.459
1.212ProPhe: 1.212 ± 0.225
6.004ProGly: 6.004 ± 0.617
1.652ProHis: 1.652 ± 0.339
1.873ProIle: 1.873 ± 0.303
2.093ProLys: 2.093 ± 0.281
4.737ProLeu: 4.737 ± 0.512
0.991ProMet: 0.991 ± 0.278
1.046ProAsn: 1.046 ± 0.34
3.305ProPro: 3.305 ± 0.583
1.652ProGln: 1.652 ± 0.297
2.919ProArg: 2.919 ± 0.459
3.36ProSer: 3.36 ± 0.525
3.25ProThr: 3.25 ± 0.46
4.241ProVal: 4.241 ± 0.475
1.102ProTrp: 1.102 ± 0.252
1.432ProTyr: 1.432 ± 0.337
0.0ProXaa: 0.0 ± 0.0
Gln
4.461GlnAla: 4.461 ± 0.533
0.386GlnCys: 0.386 ± 0.144
1.763GlnAsp: 1.763 ± 0.311
0.771GlnGlu: 0.771 ± 0.226
0.991GlnPhe: 0.991 ± 0.243
2.754GlnGly: 2.754 ± 0.343
1.046GlnHis: 1.046 ± 0.224
1.432GlnIle: 1.432 ± 0.191
0.936GlnLys: 0.936 ± 0.212
3.029GlnLeu: 3.029 ± 0.431
0.606GlnMet: 0.606 ± 0.179
0.661GlnAsn: 0.661 ± 0.165
2.093GlnPro: 2.093 ± 0.379
2.038GlnGln: 2.038 ± 0.399
2.919GlnArg: 2.919 ± 0.43
1.102GlnSer: 1.102 ± 0.267
1.763GlnThr: 1.763 ± 0.343
2.589GlnVal: 2.589 ± 0.4
1.046GlnTrp: 1.046 ± 0.235
1.102GlnTyr: 1.102 ± 0.239
0.0GlnXaa: 0.0 ± 0.0
Arg
6.995ArgAla: 6.995 ± 0.743
1.102ArgCys: 1.102 ± 0.294
4.241ArgAsp: 4.241 ± 0.557
4.516ArgGlu: 4.516 ± 0.531
2.148ArgPhe: 2.148 ± 0.302
5.177ArgGly: 5.177 ± 0.44
1.597ArgHis: 1.597 ± 0.31
2.974ArgIle: 2.974 ± 0.428
3.25ArgLys: 3.25 ± 0.412
6.444ArgLeu: 6.444 ± 0.788
1.487ArgMet: 1.487 ± 0.29
2.479ArgAsn: 2.479 ± 0.354
3.36ArgPro: 3.36 ± 0.446
2.479ArgGln: 2.479 ± 0.343
6.554ArgArg: 6.554 ± 0.723
3.25ArgSer: 3.25 ± 0.312
4.021ArgThr: 4.021 ± 0.497
5.232ArgVal: 5.232 ± 0.717
1.818ArgTrp: 1.818 ± 0.343
1.707ArgTyr: 1.707 ± 0.31
0.0ArgXaa: 0.0 ± 0.0
Ser
6.554SerAla: 6.554 ± 0.789
0.386SerCys: 0.386 ± 0.163
2.589SerAsp: 2.589 ± 0.368
3.084SerGlu: 3.084 ± 0.452
1.928SerPhe: 1.928 ± 0.298
6.004SerGly: 6.004 ± 0.844
0.936SerHis: 0.936 ± 0.249
2.203SerIle: 2.203 ± 0.372
1.212SerLys: 1.212 ± 0.285
4.241SerLeu: 4.241 ± 0.46
1.597SerMet: 1.597 ± 0.296
1.157SerAsn: 1.157 ± 0.233
2.809SerPro: 2.809 ± 0.401
1.763SerGln: 1.763 ± 0.314
3.139SerArg: 3.139 ± 0.452
2.974SerSer: 2.974 ± 0.493
2.589SerThr: 2.589 ± 0.367
4.076SerVal: 4.076 ± 0.441
0.991SerTrp: 0.991 ± 0.309
0.771SerTyr: 0.771 ± 0.19
0.0SerXaa: 0.0 ± 0.0
Thr
7.931ThrAla: 7.931 ± 0.612
0.881ThrCys: 0.881 ± 0.231
3.855ThrAsp: 3.855 ± 0.352
3.084ThrGlu: 3.084 ± 0.474
1.983ThrPhe: 1.983 ± 0.367
4.847ThrGly: 4.847 ± 0.44
1.267ThrHis: 1.267 ± 0.272
3.305ThrIle: 3.305 ± 0.481
1.432ThrLys: 1.432 ± 0.328
4.076ThrLeu: 4.076 ± 0.558
0.936ThrMet: 0.936 ± 0.251
1.542ThrAsn: 1.542 ± 0.299
4.076ThrPro: 4.076 ± 0.528
1.597ThrGln: 1.597 ± 0.284
3.415ThrArg: 3.415 ± 0.454
2.368ThrSer: 2.368 ± 0.359
3.635ThrThr: 3.635 ± 0.535
4.461ThrVal: 4.461 ± 0.434
1.102ThrTrp: 1.102 ± 0.246
1.707ThrTyr: 1.707 ± 0.317
0.0ThrXaa: 0.0 ± 0.0
Val
10.3ValAla: 10.3 ± 1.117
1.046ValCys: 1.046 ± 0.236
5.838ValAsp: 5.838 ± 0.587
5.618ValGlu: 5.618 ± 0.614
1.763ValPhe: 1.763 ± 0.342
6.169ValGly: 6.169 ± 0.588
1.928ValHis: 1.928 ± 0.352
2.754ValIle: 2.754 ± 0.354
2.974ValLys: 2.974 ± 0.386
6.059ValLeu: 6.059 ± 0.677
1.597ValMet: 1.597 ± 0.224
2.038ValAsn: 2.038 ± 0.341
4.186ValPro: 4.186 ± 0.381
2.148ValGln: 2.148 ± 0.32
4.461ValArg: 4.461 ± 0.501
3.635ValSer: 3.635 ± 0.331
5.563ValThr: 5.563 ± 0.69
5.398ValVal: 5.398 ± 0.541
1.763ValTrp: 1.763 ± 0.345
1.652ValTyr: 1.652 ± 0.329
0.0ValXaa: 0.0 ± 0.0
Trp
1.983TrpAla: 1.983 ± 0.34
0.441TrpCys: 0.441 ± 0.188
0.771TrpAsp: 0.771 ± 0.214
0.936TrpGlu: 0.936 ± 0.291
0.661TrpPhe: 0.661 ± 0.189
1.322TrpGly: 1.322 ± 0.24
0.826TrpHis: 0.826 ± 0.206
0.881TrpIle: 0.881 ± 0.209
0.441TrpLys: 0.441 ± 0.146
2.644TrpLeu: 2.644 ± 0.323
0.22TrpMet: 0.22 ± 0.115
0.551TrpAsn: 0.551 ± 0.15
0.936TrpPro: 0.936 ± 0.235
1.267TrpGln: 1.267 ± 0.243
1.873TrpArg: 1.873 ± 0.287
1.267TrpSer: 1.267 ± 0.322
1.267TrpThr: 1.267 ± 0.254
1.322TrpVal: 1.322 ± 0.267
0.661TrpTrp: 0.661 ± 0.186
0.661TrpTyr: 0.661 ± 0.202
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.534TyrAla: 2.534 ± 0.318
0.22TyrCys: 0.22 ± 0.091
1.983TyrAsp: 1.983 ± 0.432
1.212TyrGlu: 1.212 ± 0.233
0.551TyrPhe: 0.551 ± 0.157
2.038TyrGly: 2.038 ± 0.33
0.11TyrHis: 0.11 ± 0.072
0.33TyrIle: 0.33 ± 0.123
0.551TyrLys: 0.551 ± 0.17
1.873TyrLeu: 1.873 ± 0.278
0.496TyrMet: 0.496 ± 0.201
0.551TyrAsn: 0.551 ± 0.156
1.377TyrPro: 1.377 ± 0.327
0.606TyrGln: 0.606 ± 0.181
1.983TyrArg: 1.983 ± 0.392
1.046TyrSer: 1.046 ± 0.264
1.322TyrThr: 1.322 ± 0.279
1.818TyrVal: 1.818 ± 0.339
0.496TyrTrp: 0.496 ± 0.146
0.441TyrTyr: 0.441 ± 0.136
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (18157 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski