Streptococcus satellite phage Javan342
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZMD9|A0A4D5ZMD9_9VIRU Putative transcription regulator OS=Streptococcus satellite phage Javan342 OX=2558650 GN=JavanS342_0015 PE=4 SV=1
MM1 pKa = 7.75 EE2 pKa = 4.87 IVIFDD7 pKa = 3.76 SAKK10 pKa = 10.72 EE11 pKa = 3.78 LDD13 pKa = 3.63 LDD15 pKa = 3.94 AFYY18 pKa = 11.32 SEE20 pKa = 4.39 YY21 pKa = 11.12 LDD23 pKa = 3.62 VLFISNQVDD32 pKa = 3.52 EE33 pKa = 4.71 EE34 pKa = 4.2 TRR36 pKa = 11.84 AAIIEE41 pKa = 4.53 CYY43 pKa = 10.07 KK44 pKa = 10.61 KK45 pKa = 10.43 QQ46 pKa = 3.26
Molecular weight: 5.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.117
IPC2_protein 4.075
IPC_protein 3.923
Toseland 3.745
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.757
Grimsley 3.656
Solomon 3.872
Lehninger 3.821
Nozaki 4.024
DTASelect 4.164
Thurlkill 3.795
EMBOSS 3.821
Sillero 4.037
Patrickios 3.16
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.954
Protein with the highest isoelectric point:
>tr|A0A4D5ZK50|A0A4D5ZK50_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan342 OX=2558650 GN=JavanS342_0001 PE=3 SV=1
MM1 pKa = 7.62 QEE3 pKa = 3.68 LHH5 pKa = 6.17 KK6 pKa = 9.77 TNTSQVEE13 pKa = 4.2 GSLSILSDD21 pKa = 3.25 VARR24 pKa = 11.84 GKK26 pKa = 10.71 RR27 pKa = 11.84 EE28 pKa = 4.07 LKK30 pKa = 10.06 RR31 pKa = 11.84 GKK33 pKa = 10.57 AFGKK37 pKa = 10.47 RR38 pKa = 11.84 II39 pKa = 3.57
Molecular weight: 4.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.167
IPC2_protein 9.545
IPC_protein 9.736
Toseland 10.906
ProMoST 10.379
Dawson 10.95
Bjellqvist 10.54
Wikipedia 11.067
Rodwell 11.403
Grimsley 10.95
Solomon 11.052
Lehninger 11.038
Nozaki 10.862
DTASelect 10.54
Thurlkill 10.862
EMBOSS 11.286
Sillero 10.862
Patrickios 11.199
IPC_peptide 11.067
IPC2_peptide 8.829
IPC2.peptide.svr19 8.749
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16
0
16
2453
39
387
153.3
17.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.074 ± 0.6
0.571 ± 0.13
6.033 ± 0.429
8.642 ± 0.857
3.465 ± 0.435
4.117 ± 0.303
1.631 ± 0.276
7.297 ± 0.534
10.069 ± 0.647
10.599 ± 0.939
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.528 ± 0.242
5.503 ± 0.576
2.283 ± 0.198
4.77 ± 0.369
4.566 ± 0.347
5.463 ± 0.528
5.177 ± 0.589
5.055 ± 0.522
1.182 ± 0.182
4.974 ± 0.526
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here