Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosospira; Nitrosospira multiformis

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2732 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2Y597|Q2Y597_NITMU Phage-related protein OS=Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71) OX=323848 GN=Nmul_B2803 PE=4 SV=1
MM1 pKa = 7.25FSKK4 pKa = 10.47IAFTATLLLSQISLASAAIVNFDD27 pKa = 4.88DD28 pKa = 5.43LDD30 pKa = 4.52ASGGDD35 pKa = 3.62LVLDD39 pKa = 3.91TLSPYY44 pKa = 10.51QGFTWTNFSAYY55 pKa = 8.99TSIPGFPGFNNGIVSPDD72 pKa = 2.99NAAYY76 pKa = 10.46SGGEE80 pKa = 3.95SFGPIVTPVIGKK92 pKa = 9.16IEE94 pKa = 4.44ADD96 pKa = 3.65DD97 pKa = 4.58PFDD100 pKa = 4.17FVSAYY105 pKa = 10.37LGSGYY110 pKa = 10.7YY111 pKa = 10.22DD112 pKa = 4.07DD113 pKa = 6.56LSLTVQGRR121 pKa = 11.84LNGTLLFTTTLTLDD135 pKa = 3.36TAAPLFVNFGFTGINEE151 pKa = 4.55LNFFAAATGSTTDD164 pKa = 3.73PFQCGTVNCTQFTLDD179 pKa = 4.17NLTFAPGSTTPPPNPLPEE197 pKa = 4.72PSSLALLCLGGAGAWALRR215 pKa = 11.84RR216 pKa = 11.84RR217 pKa = 11.84KK218 pKa = 9.99VKK220 pKa = 10.62AA221 pKa = 3.24

Molecular weight: 23.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2Y5F5|Q2Y5F5_NITMU Uncharacterized protein OS=Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71) OX=323848 GN=Nmul_A2729 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.8TAGGAAVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.98GRR39 pKa = 11.84VRR41 pKa = 11.84LGVQQ45 pKa = 3.06

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2732

0

2732

902046

41

5216

330.2

36.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.906 ± 0.051

0.94 ± 0.017

5.075 ± 0.034

6.317 ± 0.047

3.915 ± 0.031

7.828 ± 0.051

2.327 ± 0.024

5.785 ± 0.033

4.165 ± 0.044

10.512 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.502 ± 0.025

3.419 ± 0.03

4.879 ± 0.033

3.745 ± 0.025

6.548 ± 0.036

6.063 ± 0.044

5.094 ± 0.037

6.948 ± 0.04

1.288 ± 0.017

2.743 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski