Amino acid dipepetide frequency for Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.119AlaAla: 11.119 ± 0.161	0.987AlaCys: 0.987 ± 0.036	4.86AlaAsp: 4.86 ± 0.078	6.261AlaGlu: 6.261 ± 0.1	3.646AlaPhe: 3.646 ± 0.063	8.514AlaGly: 8.514 ± 0.142	2.162AlaHis: 2.162 ± 0.061	5.682AlaIle: 5.682 ± 0.082	3.649AlaLys: 3.649 ± 0.072	10.948AlaLeu: 10.948 ± 0.135	2.643AlaMet: 2.643 ± 0.062	3.035AlaAsn: 3.035 ± 0.065	3.76AlaPro: 3.76 ± 0.073	3.75AlaGln: 3.75 ± 0.069	6.669AlaArg: 6.669 ± 0.088	5.802AlaSer: 5.802 ± 0.095	4.642AlaThr: 4.642 ± 0.084	7.116AlaVal: 7.116 ± 0.1	1.269AlaTrp: 1.269 ± 0.035	2.543AlaTyr: 2.543 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	0.87CysAla: 0.87 ± 0.036	0.119CysCys: 0.119 ± 0.014	0.533CysAsp: 0.533 ± 0.026	0.559CysGlu: 0.559 ± 0.025	0.346CysPhe: 0.346 ± 0.02	0.932CysGly: 0.932 ± 0.036	0.341CysHis: 0.341 ± 0.024	0.525CysIle: 0.525 ± 0.023	0.325CysLys: 0.325 ± 0.02	0.857CysLeu: 0.857 ± 0.034	0.194CysMet: 0.194 ± 0.015	0.33CysAsn: 0.33 ± 0.021	0.49CysPro: 0.49 ± 0.026	0.289CysGln: 0.289 ± 0.019	0.684CysArg: 0.684 ± 0.031	0.566CysSer: 0.566 ± 0.026	0.417CysThr: 0.417 ± 0.023	0.617CysVal: 0.617 ± 0.029	0.115CysTrp: 0.115 ± 0.013	0.292CysTyr: 0.292 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	4.816AspAla: 4.816 ± 0.081	0.451AspCys: 0.451 ± 0.02	2.304AspAsp: 2.304 ± 0.051	3.501AspGlu: 3.501 ± 0.07	2.296AspPhe: 2.296 ± 0.058	3.732AspGly: 3.732 ± 0.093	1.092AspHis: 1.092 ± 0.039	3.267AspIle: 3.267 ± 0.073	2.229AspLys: 2.229 ± 0.065	5.322AspLeu: 5.322 ± 0.09	1.204AspMet: 1.204 ± 0.034	1.626AspAsn: 1.626 ± 0.042	2.704AspPro: 2.704 ± 0.07	1.682AspGln: 1.682 ± 0.044	3.112AspArg: 3.112 ± 0.06	2.905AspSer: 2.905 ± 0.057	2.472AspThr: 2.472 ± 0.057	3.561AspVal: 3.561 ± 0.075	0.799AspTrp: 0.799 ± 0.032	1.681AspTyr: 1.681 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	6.587GluAla: 6.587 ± 0.101	0.509GluCys: 0.509 ± 0.023	2.754GluAsp: 2.754 ± 0.059	4.042GluGlu: 4.042 ± 0.089	2.276GluPhe: 2.276 ± 0.049	4.219GluGly: 4.219 ± 0.076	1.462GluHis: 1.462 ± 0.041	4.309GluIle: 4.309 ± 0.09	3.406GluLys: 3.406 ± 0.086	6.326GluLeu: 6.326 ± 0.094	1.733GluMet: 1.733 ± 0.048	2.349GluAsn: 2.349 ± 0.057	2.507GluPro: 2.507 ± 0.06	2.898GluGln: 2.898 ± 0.065	4.476GluArg: 4.476 ± 0.093	3.416GluSer: 3.416 ± 0.066	3.208GluThr: 3.208 ± 0.061	4.147GluVal: 4.147 ± 0.071	0.859GluTrp: 0.859 ± 0.035	1.685GluTyr: 1.685 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	3.704PheAla: 3.704 ± 0.079	0.429PheCys: 0.429 ± 0.021	2.468PheAsp: 2.468 ± 0.057	2.279PheGlu: 2.279 ± 0.051	1.722PhePhe: 1.722 ± 0.046	3.212PheGly: 3.212 ± 0.069	0.9PheHis: 0.9 ± 0.032	2.289PheIle: 2.289 ± 0.057	1.233PheLys: 1.233 ± 0.04	3.673PheLeu: 3.673 ± 0.072	0.941PheMet: 0.941 ± 0.034	1.458PheAsn: 1.458 ± 0.051	1.845PhePro: 1.845 ± 0.041	1.267PheGln: 1.267 ± 0.044	2.372PheArg: 2.372 ± 0.055	2.866PheSer: 2.866 ± 0.058	2.143PheThr: 2.143 ± 0.05	2.586PheVal: 2.586 ± 0.057	0.558PheTrp: 0.558 ± 0.03	1.209PheTyr: 1.209 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	6.67GlyAla: 6.67 ± 0.122	0.854GlyCys: 0.854 ± 0.038	3.632GlyAsp: 3.632 ± 0.069	4.829GlyGlu: 4.829 ± 0.093	3.317GlyPhe: 3.317 ± 0.071	6.38GlyGly: 6.38 ± 0.131	1.776GlyHis: 1.776 ± 0.051	5.186GlyIle: 5.186 ± 0.086	4.105GlyLys: 4.105 ± 0.076	7.763GlyLeu: 7.763 ± 0.101	2.499GlyMet: 2.499 ± 0.066	2.879GlyAsn: 2.879 ± 0.082	2.474GlyPro: 2.474 ± 0.062	2.769GlyGln: 2.769 ± 0.063	4.776GlyArg: 4.776 ± 0.075	4.787GlySer: 4.787 ± 0.107	4.325GlyThr: 4.325 ± 0.101	5.541GlyVal: 5.541 ± 0.088	1.175GlyTrp: 1.175 ± 0.04	2.548GlyTyr: 2.548 ± 0.062	0.0GlyXaa: 0.0 ± 0.0
His	2.304HisAla: 2.304 ± 0.055	0.292HisCys: 0.292 ± 0.018	1.143HisAsp: 1.143 ± 0.042	1.41HisGlu: 1.41 ± 0.033	1.005HisPhe: 1.005 ± 0.033	1.877HisGly: 1.877 ± 0.041	0.672HisHis: 0.672 ± 0.029	1.326HisIle: 1.326 ± 0.043	0.788HisLys: 0.788 ± 0.028	2.448HisLeu: 2.448 ± 0.067	0.539HisMet: 0.539 ± 0.022	0.716HisAsn: 0.716 ± 0.028	1.529HisPro: 1.529 ± 0.048	0.825HisGln: 0.825 ± 0.031	1.462HisArg: 1.462 ± 0.047	1.29HisSer: 1.29 ± 0.044	1.035HisThr: 1.035 ± 0.035	1.491HisVal: 1.491 ± 0.039	0.341HisTrp: 0.341 ± 0.02	0.773HisTyr: 0.773 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	6.255IleAla: 6.255 ± 0.089	0.55IleCys: 0.55 ± 0.026	3.335IleAsp: 3.335 ± 0.064	3.994IleGlu: 3.994 ± 0.077	2.155IlePhe: 2.155 ± 0.054	4.605IleGly: 4.605 ± 0.078	1.288IleHis: 1.288 ± 0.039	2.916IleIle: 2.916 ± 0.063	2.307IleLys: 2.307 ± 0.059	5.363IleLeu: 5.363 ± 0.087	1.152IleMet: 1.152 ± 0.036	2.112IleAsn: 2.112 ± 0.052	3.062IlePro: 3.062 ± 0.066	1.763IleGln: 1.763 ± 0.052	3.747IleArg: 3.747 ± 0.068	3.886IleSer: 3.886 ± 0.066	3.366IleThr: 3.366 ± 0.067	3.881IleVal: 3.881 ± 0.076	0.603IleTrp: 0.603 ± 0.03	1.509IleTyr: 1.509 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	3.883LysAla: 3.883 ± 0.084	0.28LysCys: 0.28 ± 0.018	2.025LysAsp: 2.025 ± 0.05	2.747LysGlu: 2.747 ± 0.085	1.265LysPhe: 1.265 ± 0.043	2.81LysGly: 2.81 ± 0.061	0.877LysHis: 0.877 ± 0.033	2.505LysIle: 2.505 ± 0.065	2.204LysLys: 2.204 ± 0.066	4.33LysLeu: 4.33 ± 0.07	1.115LysMet: 1.115 ± 0.038	1.586LysAsn: 1.586 ± 0.041	2.367LysPro: 2.367 ± 0.061	1.691LysGln: 1.691 ± 0.051	2.774LysArg: 2.774 ± 0.057	2.393LysSer: 2.393 ± 0.063	2.452LysThr: 2.452 ± 0.052	2.811LysVal: 2.811 ± 0.068	0.528LysTrp: 0.528 ± 0.025	1.0LysTyr: 1.0 ± 0.035	0.0LysXaa: 0.0 ± 0.0
Leu	11.251LeuAla: 11.251 ± 0.121	0.971LeuCys: 0.971 ± 0.034	5.573LeuAsp: 5.573 ± 0.092	6.368LeuGlu: 6.368 ± 0.09	3.897LeuPhe: 3.897 ± 0.078	7.699LeuGly: 7.699 ± 0.109	2.355LeuHis: 2.355 ± 0.049	5.598LeuIle: 5.598 ± 0.085	4.535LeuLys: 4.535 ± 0.091	11.493LeuLeu: 11.493 ± 0.18	2.482LeuMet: 2.482 ± 0.056	3.529LeuAsn: 3.529 ± 0.073	5.87LeuPro: 5.87 ± 0.081	3.806LeuGln: 3.806 ± 0.074	6.861LeuArg: 6.861 ± 0.115	6.857LeuSer: 6.857 ± 0.103	5.445LeuThr: 5.445 ± 0.079	6.87LeuVal: 6.87 ± 0.106	1.192LeuTrp: 1.192 ± 0.041	2.473LeuTyr: 2.473 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	2.377MetAla: 2.377 ± 0.051	0.162MetCys: 0.162 ± 0.013	1.195MetAsp: 1.195 ± 0.037	1.532MetGlu: 1.532 ± 0.05	0.769MetPhe: 0.769 ± 0.027	1.923MetGly: 1.923 ± 0.046	0.609MetHis: 0.609 ± 0.028	1.324MetIle: 1.324 ± 0.039	1.343MetLys: 1.343 ± 0.042	2.725MetLeu: 2.725 ± 0.061	0.677MetMet: 0.677 ± 0.025	1.03MetAsn: 1.03 ± 0.034	1.439MetPro: 1.439 ± 0.041	1.072MetGln: 1.072 ± 0.034	1.701MetArg: 1.701 ± 0.045	1.588MetSer: 1.588 ± 0.046	1.345MetThr: 1.345 ± 0.037	1.609MetVal: 1.609 ± 0.047	0.208MetTrp: 0.208 ± 0.015	0.397MetTyr: 0.397 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	3.307AsnAla: 3.307 ± 0.07	0.367AsnCys: 0.367 ± 0.025	1.657AsnAsp: 1.657 ± 0.044	1.879AsnGlu: 1.879 ± 0.048	1.411AsnPhe: 1.411 ± 0.038	2.702AsnGly: 2.702 ± 0.062	0.772AsnHis: 0.772 ± 0.031	2.036AsnIle: 2.036 ± 0.054	1.263AsnLys: 1.263 ± 0.044	3.591AsnLeu: 3.591 ± 0.07	0.746AsnMet: 0.746 ± 0.029	1.171AsnAsn: 1.171 ± 0.042	2.309AsnPro: 2.309 ± 0.054	1.203AsnGln: 1.203 ± 0.038	2.325AsnArg: 2.325 ± 0.052	1.93AsnSer: 1.93 ± 0.047	1.755AsnThr: 1.755 ± 0.054	2.29AsnVal: 2.29 ± 0.056	0.488AsnTrp: 0.488 ± 0.022	0.986AsnTyr: 0.986 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	4.909ProAla: 4.909 ± 0.087	0.411ProCys: 0.411 ± 0.024	3.108ProAsp: 3.108 ± 0.058	3.916ProGlu: 3.916 ± 0.07	1.89ProPhe: 1.89 ± 0.042	4.296ProGly: 4.296 ± 0.086	1.145ProHis: 1.145 ± 0.035	2.131ProIle: 2.131 ± 0.048	1.635ProLys: 1.635 ± 0.052	4.917ProLeu: 4.917 ± 0.072	1.049ProMet: 1.049 ± 0.029	1.428ProAsn: 1.428 ± 0.046	2.61ProPro: 2.61 ± 0.064	1.85ProGln: 1.85 ± 0.044	2.567ProArg: 2.567 ± 0.057	2.859ProSer: 2.859 ± 0.058	2.027ProThr: 2.027 ± 0.046	4.144ProVal: 4.144 ± 0.073	0.619ProTrp: 0.619 ± 0.029	1.277ProTyr: 1.277 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	3.996GlnAla: 3.996 ± 0.068	0.273GlnCys: 0.273 ± 0.017	1.654GlnAsp: 1.654 ± 0.049	2.29GlnGlu: 2.29 ± 0.054	1.324GlnPhe: 1.324 ± 0.041	2.748GlnGly: 2.748 ± 0.069	0.884GlnHis: 0.884 ± 0.034	2.158GlnIle: 2.158 ± 0.052	1.786GlnLys: 1.786 ± 0.054	3.917GlnLeu: 3.917 ± 0.071	0.963GlnMet: 0.963 ± 0.033	1.237GlnAsn: 1.237 ± 0.04	1.833GlnPro: 1.833 ± 0.049	1.79GlnGln: 1.79 ± 0.054	2.577GlnArg: 2.577 ± 0.068	2.103GlnSer: 2.103 ± 0.044	1.791GlnThr: 1.791 ± 0.04	2.701GlnVal: 2.701 ± 0.061	0.461GlnTrp: 0.461 ± 0.022	0.959GlnTyr: 0.959 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	5.686ArgAla: 5.686 ± 0.082	0.568ArgCys: 0.568 ± 0.027	3.261ArgAsp: 3.261 ± 0.068	4.667ArgGlu: 4.667 ± 0.097	2.909ArgPhe: 2.909 ± 0.055	4.126ArgGly: 4.126 ± 0.066	1.754ArgHis: 1.754 ± 0.049	4.257ArgIle: 4.257 ± 0.079	2.943ArgLys: 2.943 ± 0.058	7.064ArgLeu: 7.064 ± 0.104	1.786ArgMet: 1.786 ± 0.043	2.565ArgAsn: 2.565 ± 0.059	2.695ArgPro: 2.695 ± 0.063	2.829ArgGln: 2.829 ± 0.061	4.508ArgArg: 4.508 ± 0.077	3.496ArgSer: 3.496 ± 0.059	2.964ArgThr: 2.964 ± 0.067	4.255ArgVal: 4.255 ± 0.065	0.909ArgTrp: 0.909 ± 0.037	2.242ArgTyr: 2.242 ± 0.061	0.0ArgXaa: 0.0 ± 0.0
Ser	5.731SerAla: 5.731 ± 0.092	0.617SerCys: 0.617 ± 0.028	2.88SerAsp: 2.88 ± 0.048	3.344SerGlu: 3.344 ± 0.062	2.413SerPhe: 2.413 ± 0.058	5.893SerGly: 5.893 ± 0.101	1.45SerHis: 1.45 ± 0.039	3.408SerIle: 3.408 ± 0.063	2.083SerLys: 2.083 ± 0.054	6.342SerLeu: 6.342 ± 0.107	1.532SerMet: 1.532 ± 0.045	1.981SerAsn: 1.981 ± 0.047	3.122SerPro: 3.122 ± 0.063	2.126SerGln: 2.126 ± 0.049	4.124SerArg: 4.124 ± 0.071	4.028SerSer: 4.028 ± 0.09	3.05SerThr: 3.05 ± 0.065	4.086SerVal: 4.086 ± 0.069	0.789SerTrp: 0.789 ± 0.031	1.633SerTyr: 1.633 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	5.155ThrAla: 5.155 ± 0.092	0.458ThrCys: 0.458 ± 0.029	2.675ThrAsp: 2.675 ± 0.058	2.86ThrGlu: 2.86 ± 0.062	1.995ThrPhe: 1.995 ± 0.061	4.97ThrGly: 4.97 ± 0.097	1.221ThrHis: 1.221 ± 0.036	2.653ThrIle: 2.653 ± 0.056	1.481ThrLys: 1.481 ± 0.04	5.974ThrLeu: 5.974 ± 0.084	1.019ThrMet: 1.019 ± 0.029	1.384ThrAsn: 1.384 ± 0.042	2.928ThrPro: 2.928 ± 0.065	1.697ThrGln: 1.697 ± 0.043	3.156ThrArg: 3.156 ± 0.063	2.999ThrSer: 2.999 ± 0.065	2.488ThrThr: 2.488 ± 0.062	3.923ThrVal: 3.923 ± 0.069	0.58ThrTrp: 0.58 ± 0.027	1.327ThrTyr: 1.327 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	7.025ValAla: 7.025 ± 0.111	0.694ValCys: 0.694 ± 0.025	3.616ValAsp: 3.616 ± 0.064	4.473ValGlu: 4.473 ± 0.073	2.747ValPhe: 2.747 ± 0.053	4.565ValGly: 4.565 ± 0.087	1.442ValHis: 1.442 ± 0.037	4.27ValIle: 4.27 ± 0.065	2.869ValLys: 2.869 ± 0.068	7.369ValLeu: 7.369 ± 0.098	1.885ValMet: 1.885 ± 0.049	2.426ValAsn: 2.426 ± 0.051	3.344ValPro: 3.344 ± 0.061	2.323ValGln: 2.323 ± 0.05	4.421ValArg: 4.421 ± 0.071	4.38ValSer: 4.38 ± 0.069	4.021ValThr: 4.021 ± 0.071	5.198ValVal: 5.198 ± 0.091	0.763ValTrp: 0.763 ± 0.032	1.651ValTyr: 1.651 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.896TrpAla: 0.896 ± 0.031	0.111TrpCys: 0.111 ± 0.012	0.622TrpAsp: 0.622 ± 0.028	0.693TrpGlu: 0.693 ± 0.029	0.548TrpPhe: 0.548 ± 0.025	0.858TrpGly: 0.858 ± 0.036	0.381TrpHis: 0.381 ± 0.02	0.712TrpIle: 0.712 ± 0.03	0.547TrpLys: 0.547 ± 0.028	1.663TrpLeu: 1.663 ± 0.049	0.356TrpMet: 0.356 ± 0.021	0.506TrpAsn: 0.506 ± 0.025	0.518TrpPro: 0.518 ± 0.021	0.647TrpGln: 0.647 ± 0.027	1.021TrpArg: 1.021 ± 0.036	0.752TrpSer: 0.752 ± 0.031	0.617TrpThr: 0.617 ± 0.028	0.859TrpVal: 0.859 ± 0.031	0.196TrpTrp: 0.196 ± 0.017	0.379TrpTyr: 0.379 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.518TyrAla: 2.518 ± 0.057	0.302TyrCys: 0.302 ± 0.019	1.459TyrAsp: 1.459 ± 0.054	1.523TyrGlu: 1.523 ± 0.042	1.223TyrPhe: 1.223 ± 0.041	2.223TyrGly: 2.223 ± 0.057	0.684TyrHis: 0.684 ± 0.031	1.295TyrIle: 1.295 ± 0.037	0.915TyrLys: 0.915 ± 0.037	3.04TyrLeu: 3.04 ± 0.064	0.508TyrMet: 0.508 ± 0.024	0.839TyrAsn: 0.839 ± 0.031	1.384TyrPro: 1.384 ± 0.042	1.168TyrGln: 1.168 ± 0.033	2.151TyrArg: 2.151 ± 0.053	1.732TyrSer: 1.732 ± 0.042	1.379TyrThr: 1.379 ± 0.042	1.794TyrVal: 1.794 ± 0.044	0.428TyrTrp: 0.428 ± 0.023	0.868TyrTyr: 0.868 ± 0.035	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2732 proteins (902046 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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