Paludibacter propionicigenes (strain DSM 17365 / JCM 13257 / WB4)
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3020 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E4T8D3|E4T8D3_PALPW Phosphatidate cytidylyltransferase OS=Paludibacter propionicigenes (strain DSM 17365 / JCM 13257 / WB4) OX=694427 GN=Palpr_2848 PE=4 SV=1
MM1 pKa = 7.39 NEE3 pKa = 3.74 SNYY6 pKa = 10.62 KK7 pKa = 8.53 YY8 pKa = 9.04 TATLLLPEE16 pKa = 5.58 DD17 pKa = 4.47 GKK19 pKa = 10.82 QYY21 pKa = 8.39 WTDD24 pKa = 2.86 IMQGRR29 pKa = 11.84 AAVPEE34 pKa = 5.07 DD35 pKa = 3.38 ITVGSPVVMATALYY49 pKa = 9.36 TDD51 pKa = 3.65 GTFVVAGVSKK61 pKa = 11.05 SKK63 pKa = 11.18 DD64 pKa = 3.2 PTDD67 pKa = 3.75 YY68 pKa = 11.14 NIVFAWVYY76 pKa = 10.76 DD77 pKa = 3.67 QDD79 pKa = 4.01 GNKK82 pKa = 9.74 YY83 pKa = 9.49 PGWPIDD89 pKa = 3.81 LSDD92 pKa = 4.33 NEE94 pKa = 4.57 DD95 pKa = 3.49 FFVNSIAFSPTDD107 pKa = 3.72 ADD109 pKa = 3.62 EE110 pKa = 4.91 ADD112 pKa = 3.58 YY113 pKa = 11.62 LLIVSEE119 pKa = 4.49 AKK121 pKa = 10.24 VV122 pKa = 3.09
Molecular weight: 13.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.77
IPC_protein 3.757
Toseland 3.528
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.719
Rodwell 3.579
Grimsley 3.439
Solomon 3.745
Lehninger 3.694
Nozaki 3.884
DTASelect 4.139
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.872
Patrickios 1.888
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|E4T6U9|E4T6U9_PALPW Helix-turn-helix domain protein OS=Paludibacter propionicigenes (strain DSM 17365 / JCM 13257 / WB4) OX=694427 GN=Palpr_2307 PE=4 SV=1
MM1 pKa = 7.63 IFLNQLIIHH10 pKa = 6.55 HH11 pKa = 5.39 QTHH14 pKa = 6.25 LQRR17 pKa = 11.84 NNTASARR24 pKa = 11.84 LYY26 pKa = 10.61 LVGMLKK32 pKa = 10.4 RR33 pKa = 11.84 NRR35 pKa = 11.84 RR36 pKa = 11.84 LVDD39 pKa = 3.35 GLPQDD44 pKa = 4.77 RR45 pKa = 11.84 IRR47 pKa = 11.84 TAAEE51 pKa = 4.83 FYY53 pKa = 10.43 RR54 pKa = 11.84 KK55 pKa = 9.87 LEE57 pKa = 3.67 RR58 pKa = 11.84 RR59 pKa = 11.84 RR60 pKa = 11.84 YY61 pKa = 9.16 MEE63 pKa = 4.02 INKK66 pKa = 7.98 NTKK69 pKa = 9.07 NICSIVCSYY78 pKa = 11.84 ANIGLISHH86 pKa = 6.36 TVLVKK91 pKa = 10.7 HH92 pKa = 5.58 STCNLSNSMIISLPGLPYY110 pKa = 10.47 GQHH113 pKa = 4.17 TWGAFSIYY121 pKa = 9.66 IPRR124 pKa = 11.84 ILPSDD129 pKa = 3.67 FHH131 pKa = 7.17 LALL134 pKa = 4.42
Molecular weight: 15.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.319
IPC2_protein 9.765
IPC_protein 10.467
Toseland 10.248
ProMoST 10.072
Dawson 10.482
Bjellqvist 10.233
Wikipedia 10.701
Rodwell 10.672
Grimsley 10.57
Solomon 10.54
Lehninger 10.496
Nozaki 10.292
DTASelect 10.218
Thurlkill 10.321
EMBOSS 10.672
Sillero 10.409
Patrickios 10.292
IPC_peptide 10.526
IPC2_peptide 9.399
IPC2.peptide.svr19 8.378
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3020
0
3020
1067089
31
5983
353.3
39.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.854 ± 0.045
0.967 ± 0.015
5.235 ± 0.033
5.88 ± 0.059
4.973 ± 0.04
6.52 ± 0.063
1.715 ± 0.02
7.673 ± 0.048
7.344 ± 0.056
9.262 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.389 ± 0.027
5.822 ± 0.061
3.548 ± 0.024
3.425 ± 0.029
3.79 ± 0.038
6.833 ± 0.064
5.93 ± 0.081
6.545 ± 0.032
1.093 ± 0.02
4.202 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here