Pygoscelis adeliae papillomavirus 1
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X2JJ85|X2JJ85_9PAPI Major capsid protein L1 OS=Pygoscelis adeliae papillomavirus 1 OX=1480065 GN=L1 PE=3 SV=1
MM1 pKa = 7.47 LTRR4 pKa = 11.84 DD5 pKa = 3.63 PSGLRR10 pKa = 11.84 PRR12 pKa = 11.84 PFKK15 pKa = 11.23 DD16 pKa = 3.35 CDD18 pKa = 3.76 DD19 pKa = 5.08 GEE21 pKa = 4.59 LSTTEE26 pKa = 3.94 EE27 pKa = 4.82 LEE29 pKa = 3.66 VDD31 pKa = 4.39 LYY33 pKa = 11.72 VDD35 pKa = 4.27 IIEE38 pKa = 5.36 DD39 pKa = 3.7 SSSDD43 pKa = 3.65 DD44 pKa = 3.95 DD45 pKa = 5.26 SDD47 pKa = 4.09 FEE49 pKa = 4.88 GYY51 pKa = 10.27 YY52 pKa = 10.8 DD53 pKa = 3.87 CTLEE57 pKa = 4.33 TLMCQEE63 pKa = 5.19 GPLSDD68 pKa = 4.14 SSSDD72 pKa = 3.53 SDD74 pKa = 3.98 SGADD78 pKa = 3.66 NEE80 pKa = 5.0 APPPPPPGAPQPLPHH95 pKa = 7.94 VEE97 pKa = 4.27 DD98 pKa = 3.72 PTIEE102 pKa = 4.21 EE103 pKa = 4.34 LLQEE107 pKa = 4.51 FGGNNDD113 pKa = 3.75 NDD115 pKa = 3.73 QEE117 pKa = 4.46 EE118 pKa = 4.92 CGWTCYY124 pKa = 10.05 FCEE127 pKa = 5.14 GPLNPTEE134 pKa = 4.15 CFLYY138 pKa = 10.43 GAFDD142 pKa = 3.92 EE143 pKa = 4.45 EE144 pKa = 4.94 HH145 pKa = 6.75 SKK147 pKa = 11.15 GMCNSCYY154 pKa = 10.66 VSTLSDD160 pKa = 3.54 FEE162 pKa = 4.33 EE163 pKa = 4.45
Molecular weight: 18.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.02
IPC2_protein 3.401
IPC_protein 3.401
Toseland 3.198
ProMoST 3.567
Dawson 3.401
Bjellqvist 3.592
Wikipedia 3.325
Rodwell 3.236
Grimsley 3.109
Solomon 3.376
Lehninger 3.338
Nozaki 3.516
DTASelect 3.719
Thurlkill 3.249
EMBOSS 3.338
Sillero 3.528
Patrickios 0.006
IPC_peptide 3.376
IPC2_peptide 3.503
IPC2.peptide.svr19 3.612
Protein with the highest isoelectric point:
>tr|X2JSD9|X2JSD9_9PAPI E9 OS=Pygoscelis adeliae papillomavirus 1 OX=1480065 PE=4 SV=1
MM1 pKa = 6.97 PTIYY5 pKa = 10.46 YY6 pKa = 8.62 PNWEE10 pKa = 4.25 TVYY13 pKa = 10.93 LIRR16 pKa = 11.84 DD17 pKa = 3.93 PEE19 pKa = 4.25 NLPMDD24 pKa = 4.1 GMTSVTLRR32 pKa = 11.84 TNSDD36 pKa = 3.33 QQSWRR41 pKa = 11.84 CCNLALPIFLKK52 pKa = 10.68 RR53 pKa = 11.84 AGDD56 pKa = 3.95 GLRR59 pKa = 11.84 LRR61 pKa = 11.84 CIDD64 pKa = 3.27 QHH66 pKa = 7.66 LPTLTRR72 pKa = 11.84 RR73 pKa = 11.84 ITGTADD79 pKa = 4.7 RR80 pKa = 11.84 IPTQDD85 pKa = 2.68 TRR87 pKa = 11.84 LDD89 pKa = 3.65 VLVDD93 pKa = 3.91 LGWRR97 pKa = 11.84 TEE99 pKa = 3.89 YY100 pKa = 11.19 LLRR103 pKa = 11.84 TRR105 pKa = 11.84 LPWNTCTVITPTEE118 pKa = 4.17 NLCSLYY124 pKa = 10.55 QLLGPSTQEE133 pKa = 3.33 AAEE136 pKa = 4.03 RR137 pKa = 11.84 RR138 pKa = 11.84 RR139 pKa = 11.84 DD140 pKa = 3.65 LMVALLEE147 pKa = 4.65 LNTEE151 pKa = 3.79 RR152 pKa = 11.84 RR153 pKa = 11.84 QRR155 pKa = 11.84 HH156 pKa = 4.56 LRR158 pKa = 11.84 RR159 pKa = 11.84 GRR161 pKa = 11.84 MRR163 pKa = 11.84 GMSYY167 pKa = 11.17 YY168 pKa = 10.52 NAYY171 pKa = 10.12
Molecular weight: 20.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.813
IPC2_protein 8.624
IPC_protein 9.063
Toseland 8.565
ProMoST 9.077
Dawson 9.107
Bjellqvist 9.326
Wikipedia 9.224
Rodwell 9.077
Grimsley 8.639
Solomon 9.282
Lehninger 9.253
Nozaki 9.341
DTASelect 9.107
Thurlkill 9.077
EMBOSS 9.224
Sillero 9.355
Patrickios 4.431
IPC_peptide 9.268
IPC2_peptide 8.931
IPC2.peptide.svr19 8.408
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2564
96
676
366.3
41.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.889 ± 0.632
2.34 ± 0.618
7.098 ± 0.636
5.694 ± 0.919
2.886 ± 0.37
7.566 ± 1.112
2.184 ± 0.354
3.861 ± 0.382
2.925 ± 0.805
9.36 ± 0.529
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.911 ± 0.293
3.666 ± 0.683
8.073 ± 0.915
3.939 ± 0.605
7.722 ± 0.753
6.708 ± 0.542
7.566 ± 0.722
5.148 ± 0.523
1.599 ± 0.252
3.861 ± 0.297
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here