Sendai virus (strain Hamamatsu) (SeV)
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9DUE0-2|C-2_SENDA Isoform of Q9DUE0 Isoform C of Protein C' OS=Sendai virus (strain Hamamatsu) OX=302271 GN=P/V/C PE=4 SV=3
MM1 pKa = 7.72 AGLLSTFDD9 pKa = 3.61 TFSSRR14 pKa = 11.84 RR15 pKa = 11.84 SEE17 pKa = 4.54 SINKK21 pKa = 9.79 SGGGAVIPGQRR32 pKa = 11.84 STVSVFVLGPSVTDD46 pKa = 3.97 DD47 pKa = 3.48 ADD49 pKa = 3.63 KK50 pKa = 11.51 LFIATTFLAHH60 pKa = 7.37 SLDD63 pKa = 3.34 TDD65 pKa = 3.7 KK66 pKa = 11.24 QHH68 pKa = 5.75 SQRR71 pKa = 11.84 GGFLVSLLAMAYY83 pKa = 9.77 SSPEE87 pKa = 3.87 LYY89 pKa = 9.56 LTTNGVNADD98 pKa = 3.69 VKK100 pKa = 10.02 YY101 pKa = 10.81 VIYY104 pKa = 10.7 NIEE107 pKa = 4.31 KK108 pKa = 10.15 DD109 pKa = 3.6 PKK111 pKa = 8.86 RR112 pKa = 11.84 TKK114 pKa = 10.08 TDD116 pKa = 2.85 GFIVKK121 pKa = 8.33 TRR123 pKa = 11.84 DD124 pKa = 3.0 MEE126 pKa = 4.37 YY127 pKa = 10.88 EE128 pKa = 4.02 RR129 pKa = 11.84 TTEE132 pKa = 3.92 WLFGPMVNKK141 pKa = 10.47 SPLFQGQRR149 pKa = 11.84 DD150 pKa = 3.65 AADD153 pKa = 4.71 PDD155 pKa = 4.11 TLLQIYY161 pKa = 8.23 GYY163 pKa = 7.7 PACLGAIIVQVWIVLVKK180 pKa = 10.76 AITSSAGLRR189 pKa = 11.84 KK190 pKa = 10.19 GFFNRR195 pKa = 11.84 LEE197 pKa = 4.14 AFRR200 pKa = 11.84 QDD202 pKa = 3.37 GTVKK206 pKa = 10.4 GALVFTGEE214 pKa = 4.37 TVEE217 pKa = 5.1 GIGSVMRR224 pKa = 11.84 SQQSLVSLMVEE235 pKa = 4.16 TLVTMNTARR244 pKa = 11.84 SDD246 pKa = 3.53 LTTLEE251 pKa = 4.27 KK252 pKa = 10.74 NIQIVGNYY260 pKa = 8.94 IRR262 pKa = 11.84 DD263 pKa = 3.52 AGLASFMNTIKK274 pKa = 11.03 YY275 pKa = 10.01 GVEE278 pKa = 3.61 TKK280 pKa = 9.64 MAALTLSNLRR290 pKa = 11.84 PDD292 pKa = 3.45 INKK295 pKa = 9.15 LRR297 pKa = 11.84 SLIDD301 pKa = 3.3 TYY303 pKa = 11.25 LSKK306 pKa = 11.09 GPRR309 pKa = 11.84 APFICILKK317 pKa = 10.47 DD318 pKa = 3.39 PVHH321 pKa = 7.11 GEE323 pKa = 4.29 FAPGNYY329 pKa = 7.59 PALWSYY335 pKa = 11.87 AMGVAVVQNKK345 pKa = 9.23 AMQQYY350 pKa = 7.92 VTGRR354 pKa = 11.84 TYY356 pKa = 11.73 LDD358 pKa = 3.07 MEE360 pKa = 4.5 MFLLGQAVAKK370 pKa = 10.25 DD371 pKa = 3.9 AEE373 pKa = 4.71 SKK375 pKa = 10.5 ISSALEE381 pKa = 4.16 DD382 pKa = 3.8 EE383 pKa = 5.42 LGVTDD388 pKa = 3.67 TAKK391 pKa = 10.49 EE392 pKa = 3.98 RR393 pKa = 11.84 LRR395 pKa = 11.84 HH396 pKa = 5.46 HH397 pKa = 7.16 LANLSGGDD405 pKa = 3.73 GAYY408 pKa = 10.07 HH409 pKa = 6.96 KK410 pKa = 8.27 PTGGGAIEE418 pKa = 4.23 VALDD422 pKa = 3.43 NADD425 pKa = 3.3 IDD427 pKa = 4.61 LEE429 pKa = 4.57 PEE431 pKa = 3.58 AHH433 pKa = 6.61 TDD435 pKa = 2.8 QDD437 pKa = 3.45 ARR439 pKa = 11.84 GWGGDD444 pKa = 3.45 SGDD447 pKa = 2.92 RR448 pKa = 11.84 WARR451 pKa = 11.84 STSSGHH457 pKa = 7.13 FITLHH462 pKa = 4.9 GAEE465 pKa = 4.1 RR466 pKa = 11.84 LEE468 pKa = 4.51 EE469 pKa = 4.07 EE470 pKa = 4.7 TNDD473 pKa = 3.59 EE474 pKa = 4.17 DD475 pKa = 4.66 VSDD478 pKa = 3.67 IEE480 pKa = 4.26 RR481 pKa = 11.84 RR482 pKa = 11.84 IARR485 pKa = 11.84 RR486 pKa = 11.84 LAEE489 pKa = 4.24 RR490 pKa = 11.84 RR491 pKa = 11.84 QEE493 pKa = 3.99 DD494 pKa = 3.76 ATTHH498 pKa = 5.81 EE499 pKa = 4.75 DD500 pKa = 3.0 EE501 pKa = 4.6 GRR503 pKa = 11.84 NNGVDD508 pKa = 3.44 HH509 pKa = 7.73 DD510 pKa = 4.74 EE511 pKa = 4.59 EE512 pKa = 6.63 DD513 pKa = 4.03 DD514 pKa = 3.75 AAAAAGMGGII524 pKa = 4.58
Molecular weight: 57.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.028
IPC2_protein 5.003
IPC_protein 4.978
Toseland 4.902
ProMoST 5.118
Dawson 4.965
Bjellqvist 5.105
Wikipedia 4.851
Rodwell 4.876
Grimsley 4.825
Solomon 4.965
Lehninger 4.927
Nozaki 5.092
DTASelect 5.27
Thurlkill 4.902
EMBOSS 4.902
Sillero 5.156
Patrickios 4.228
IPC_peptide 4.978
IPC2_peptide 5.156
IPC2.peptide.svr19 5.094
Protein with the highest isoelectric point:
>sp|Q9DUD8|L_SENDA RNA-directed RNA polymerase L OS=Sendai virus (strain Hamamatsu) OX=302271 GN=L PE=3 SV=1
MM1 pKa = 7.6 ADD3 pKa = 3.36 IYY5 pKa = 10.85 RR6 pKa = 11.84 FPKK9 pKa = 10.59 FSYY12 pKa = 9.74 EE13 pKa = 4.12 DD14 pKa = 3.44 NGTVEE19 pKa = 4.59 PLPLRR24 pKa = 11.84 TGPDD28 pKa = 3.01 KK29 pKa = 10.99 KK30 pKa = 10.56 AIPHH34 pKa = 5.1 IRR36 pKa = 11.84 IVKK39 pKa = 10.01 VGDD42 pKa = 3.63 PPKK45 pKa = 10.69 HH46 pKa = 4.84 GVRR49 pKa = 11.84 YY50 pKa = 10.03 LDD52 pKa = 3.96 LLLLGFFEE60 pKa = 4.94 TPKK63 pKa = 9.29 QTASLGSVSDD73 pKa = 3.85 LTEE76 pKa = 3.71 HH77 pKa = 6.4 TGYY80 pKa = 10.33 SICGSGSLPIGVAKK94 pKa = 10.31 YY95 pKa = 10.04 HH96 pKa = 6.94 GSDD99 pKa = 3.34 QEE101 pKa = 4.2 LLKK104 pKa = 11.05 ACTDD108 pKa = 3.21 LRR110 pKa = 11.84 ITVRR114 pKa = 11.84 RR115 pKa = 11.84 TVRR118 pKa = 11.84 AGEE121 pKa = 4.17 MIVYY125 pKa = 8.62 MVDD128 pKa = 3.46 SIGAPLLPWSGRR140 pKa = 11.84 LRR142 pKa = 11.84 QGMIFNANKK151 pKa = 9.77 VALAPQCLPVDD162 pKa = 3.23 KK163 pKa = 10.48 DD164 pKa = 2.96 IRR166 pKa = 11.84 FRR168 pKa = 11.84 VVFVNGTSLGAITIAKK184 pKa = 9.25 IPKK187 pKa = 8.6 TLADD191 pKa = 3.79 LALPNSISVNLLVTLKK207 pKa = 10.09 TGIPTEE213 pKa = 4.17 QKK215 pKa = 10.68 GVLPVLDD222 pKa = 4.08 DD223 pKa = 3.73 QGEE226 pKa = 4.28 KK227 pKa = 10.49 KK228 pKa = 10.88 LNFMVHH234 pKa = 6.52 LGLIRR239 pKa = 11.84 RR240 pKa = 11.84 KK241 pKa = 9.27 VGKK244 pKa = 9.46 IYY246 pKa = 10.67 SVEE249 pKa = 4.02 YY250 pKa = 9.84 CKK252 pKa = 11.04 SKK254 pKa = 10.18 IEE256 pKa = 3.93 RR257 pKa = 11.84 MRR259 pKa = 11.84 LIFSLGLIGGISFHH273 pKa = 5.67 VQVTGTLSKK282 pKa = 10.13 TFMSQLAWKK291 pKa = 10.01 RR292 pKa = 11.84 AVCFPLMDD300 pKa = 4.24 VNPHH304 pKa = 5.39 MNLVIWAASVEE315 pKa = 4.28 ITDD318 pKa = 3.38 VDD320 pKa = 4.49 AVFQPAIPRR329 pKa = 11.84 DD330 pKa = 3.38 FRR332 pKa = 11.84 YY333 pKa = 10.38 YY334 pKa = 10.21 PNVVAKK340 pKa = 10.68 NIGRR344 pKa = 11.84 IRR346 pKa = 11.84 KK347 pKa = 8.53 LL348 pKa = 3.16
Molecular weight: 38.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.064
IPC2_protein 9.033
IPC_protein 8.946
Toseland 9.765
ProMoST 9.443
Dawson 9.984
Bjellqvist 9.663
Wikipedia 10.131
Rodwell 10.394
Grimsley 10.043
Solomon 10.014
Lehninger 9.97
Nozaki 9.838
DTASelect 9.633
Thurlkill 9.838
EMBOSS 10.175
Sillero 9.911
Patrickios 9.545
IPC_peptide 10.014
IPC2_peptide 8.302
IPC2.peptide.svr19 7.913
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
2
10
5777
181
2228
577.7
64.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.007 ± 0.58
1.489 ± 0.305
5.816 ± 0.343
6.959 ± 0.717
2.51 ± 0.486
6.439 ± 0.554
1.991 ± 0.089
6.612 ± 0.582
5.383 ± 0.354
9.936 ± 0.889
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.181 ± 0.195
3.981 ± 0.308
4.795 ± 0.532
3.566 ± 0.379
6.664 ± 0.528
8.343 ± 0.51
6.699 ± 0.344
6.266 ± 0.399
1.177 ± 0.196
3.185 ± 0.447
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here