Hubei sobemo-like virus 11

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KES0|A0A1L3KES0_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 11 OX=1923196 PE=4 SV=1
MM1 pKa = 7.53LKK3 pKa = 10.46NVVLEE8 pKa = 4.12RR9 pKa = 11.84MHH11 pKa = 7.2RR12 pKa = 11.84LINGADD18 pKa = 3.51VFDD21 pKa = 6.09DD22 pKa = 3.28IQVFIKK28 pKa = 10.43QEE30 pKa = 3.65PHH32 pKa = 6.54KK33 pKa = 10.45LRR35 pKa = 11.84KK36 pKa = 9.22IEE38 pKa = 3.86EE39 pKa = 4.06GRR41 pKa = 11.84FRR43 pKa = 11.84LISGVSFVDD52 pKa = 4.86GIIDD56 pKa = 4.23RR57 pKa = 11.84IVFGWLADD65 pKa = 3.49AAMNAIGQTPALLGWNPLFGGDD87 pKa = 3.46RR88 pKa = 11.84LLFSKK93 pKa = 10.31IQAPFFSLDD102 pKa = 3.01KK103 pKa = 11.15SLWDD107 pKa = 3.22WTVPAWLVDD116 pKa = 3.46LWLQFILGLYY126 pKa = 10.0HH127 pKa = 7.75DD128 pKa = 4.1MPEE131 pKa = 3.94WLEE134 pKa = 3.77LLIVKK139 pKa = 9.71RR140 pKa = 11.84FNILFAKK147 pKa = 10.27SRR149 pKa = 11.84FRR151 pKa = 11.84FPDD154 pKa = 3.42GCVIEE159 pKa = 4.51QKK161 pKa = 10.65FSGIMKK167 pKa = 9.7SGCFLTILLNSVGQYY182 pKa = 10.43LVDD185 pKa = 5.29RR186 pKa = 11.84IVSYY190 pKa = 11.09NLNIPTGEE198 pKa = 4.01ILCYY202 pKa = 10.58GDD204 pKa = 4.61DD205 pKa = 4.46TIQEE209 pKa = 4.17VPDD212 pKa = 3.5NVEE215 pKa = 4.01NYY217 pKa = 9.4ISAVRR222 pKa = 11.84ALGFLPKK229 pKa = 10.54DD230 pKa = 3.71PIVSNVAEE238 pKa = 4.09FVGFVMDD245 pKa = 3.73GRR247 pKa = 11.84KK248 pKa = 9.57VIPAYY253 pKa = 10.21YY254 pKa = 9.9KK255 pKa = 9.63KK256 pKa = 9.85HH257 pKa = 6.08LYY259 pKa = 9.39NLRR262 pKa = 11.84HH263 pKa = 6.39LDD265 pKa = 3.59EE266 pKa = 4.96KK267 pKa = 11.07VAEE270 pKa = 4.27ATLEE274 pKa = 4.0NYY276 pKa = 10.03QILYY280 pKa = 10.43ADD282 pKa = 4.21SEE284 pKa = 4.41EE285 pKa = 4.26LLPVIQAEE293 pKa = 4.26LTQRR297 pKa = 11.84NPTRR301 pKa = 11.84VIPRR305 pKa = 11.84VALQRR310 pKa = 11.84VVRR313 pKa = 11.84GG314 pKa = 3.54

Molecular weight: 36.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KES0|A0A1L3KES0_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 11 OX=1923196 PE=4 SV=1
MM1 pKa = 7.81ALRR4 pKa = 11.84FVCEE8 pKa = 3.73VLFWILFMVFKK19 pKa = 10.45VLWRR23 pKa = 11.84FEE25 pKa = 4.28LGRR28 pKa = 11.84CILAFLSVFGLVLFWNRR45 pKa = 11.84VIDD48 pKa = 4.29LLWLVWVILSGLWRR62 pKa = 11.84VKK64 pKa = 9.9PVAVAPEE71 pKa = 4.19PVSPASMLLWWFIIVALLGILMLTYY96 pKa = 11.19LLFLWVFVKK105 pKa = 10.57IKK107 pKa = 10.27SAIVGKK113 pKa = 10.02QIEE116 pKa = 4.37KK117 pKa = 10.34FAFEE121 pKa = 5.0RR122 pKa = 11.84YY123 pKa = 8.54QEE125 pKa = 4.19GSPYY129 pKa = 10.8LSAKK133 pKa = 10.17CPDD136 pKa = 3.69VQCEE140 pKa = 3.97VWVSYY145 pKa = 11.2DD146 pKa = 3.78GDD148 pKa = 3.8WLKK151 pKa = 10.99SGQAFRR157 pKa = 11.84VGDD160 pKa = 3.66KK161 pKa = 10.84LITAGHH167 pKa = 5.19VVRR170 pKa = 11.84EE171 pKa = 3.96ARR173 pKa = 11.84QAKK176 pKa = 8.51FVSGAGEE183 pKa = 4.07VFVDD187 pKa = 3.34VDD189 pKa = 3.81SFAEE193 pKa = 3.95IEE195 pKa = 4.08GDD197 pKa = 3.91VVFVRR202 pKa = 11.84LSNLDD207 pKa = 3.12ITTLALKK214 pKa = 9.94SAKK217 pKa = 9.07FANVGCDD224 pKa = 3.03EE225 pKa = 4.57GKK227 pKa = 8.85GTFCTVQAFGRR238 pKa = 11.84MSMGMVEE245 pKa = 4.41SFPAFGFCKK254 pKa = 9.51YY255 pKa = 9.07TGSTDD260 pKa = 3.12HH261 pKa = 6.65GFSGAPYY268 pKa = 9.59LVNRR272 pKa = 11.84TVFGVHH278 pKa = 6.77IGTQGVNLGYY288 pKa = 10.6EE289 pKa = 4.32GAYY292 pKa = 9.89VAACLRR298 pKa = 11.84SRR300 pKa = 11.84NEE302 pKa = 3.76DD303 pKa = 3.39SEE305 pKa = 4.86EE306 pKa = 4.01YY307 pKa = 11.05LMKK310 pKa = 10.27QVKK313 pKa = 9.58KK314 pKa = 10.12GRR316 pKa = 11.84GVARR320 pKa = 11.84QSPYY324 pKa = 11.3DD325 pKa = 3.81PDD327 pKa = 3.35EE328 pKa = 4.09VQIQVGGKK336 pKa = 8.41YY337 pKa = 7.6FTMSRR342 pKa = 11.84DD343 pKa = 3.48AYY345 pKa = 9.57EE346 pKa = 3.96QAQEE350 pKa = 3.77EE351 pKa = 4.53MEE353 pKa = 4.04RR354 pKa = 11.84DD355 pKa = 3.87FEE357 pKa = 5.28RR358 pKa = 11.84KK359 pKa = 9.38FDD361 pKa = 4.53DD362 pKa = 5.33DD363 pKa = 3.74SGRR366 pKa = 11.84WGGGKK371 pKa = 9.84NYY373 pKa = 8.57HH374 pKa = 5.44TGKK377 pKa = 9.91NRR379 pKa = 11.84GGRR382 pKa = 11.84LVRR385 pKa = 11.84EE386 pKa = 4.26SALEE390 pKa = 3.79RR391 pKa = 11.84SEE393 pKa = 4.28PLPTFDD399 pKa = 5.93DD400 pKa = 4.1VQSEE404 pKa = 4.28PKK406 pKa = 10.14NVGRR410 pKa = 11.84IEE412 pKa = 4.01NAGVSISPKK421 pKa = 10.29KK422 pKa = 8.27PASWYY427 pKa = 8.77EE428 pKa = 3.82DD429 pKa = 2.99QEE431 pKa = 4.74VFVRR435 pKa = 11.84KK436 pKa = 9.35PNIQIGPSTSTPSTTIPVTVRR457 pKa = 11.84PDD459 pKa = 3.26MVLPEE464 pKa = 4.73SIAVPRR470 pKa = 11.84RR471 pKa = 11.84NPSSPTSKK479 pKa = 10.15NAKK482 pKa = 8.99KK483 pKa = 9.61QAKK486 pKa = 9.12ALQRR490 pKa = 11.84EE491 pKa = 4.4KK492 pKa = 10.98LRR494 pKa = 11.84SEE496 pKa = 5.07LLTQLGKK503 pKa = 10.72ALGIGDD509 pKa = 4.54RR510 pKa = 11.84EE511 pKa = 3.89ILKK514 pKa = 10.86KK515 pKa = 10.73NFSSSSNEE523 pKa = 3.84SLKK526 pKa = 10.84EE527 pKa = 3.69LLGSVTSKK535 pKa = 10.74PVV537 pKa = 2.72

Molecular weight: 60.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

851

314

537

425.5

48.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.228 ± 0.282

1.293 ± 0.192

5.17 ± 0.683

6.228 ± 0.464

5.993 ± 0.033

7.638 ± 0.723

1.058 ± 0.305

5.758 ± 1.437

5.875 ± 0.626

10.223 ± 1.252

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.998 ± 0.049

3.408 ± 0.599

4.7 ± 0.044

3.643 ± 0.102

5.993 ± 0.033

6.58 ± 1.39

3.408 ± 0.49

9.636 ± 0.409

2.35 ± 0.069

2.82 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski