Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae; Halothermothrix; Halothermothrix orenii

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2324 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8D1W4|B8D1W4_HALOH Radical SAM domain protein OS=Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) OX=373903 GN=Hore_04330 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.07KK3 pKa = 10.64LSILAILLVGLLVLAGCSKK22 pKa = 10.78DD23 pKa = 4.05ANDD26 pKa = 3.22VNSAVKK32 pKa = 8.83HH33 pKa = 4.98TLTVTVTDD41 pKa = 4.18EE42 pKa = 3.91ATQAAIEE49 pKa = 4.41GATVTVDD56 pKa = 3.42EE57 pKa = 4.86ASKK60 pKa = 7.91TTDD63 pKa = 3.11ANGVAQFEE71 pKa = 4.84LTDD74 pKa = 3.46GTYY77 pKa = 10.44DD78 pKa = 3.09ITVTAEE84 pKa = 4.31GYY86 pKa = 8.48EE87 pKa = 4.32SKK89 pKa = 10.63SGSVTIDD96 pKa = 3.41GKK98 pKa = 10.84DD99 pKa = 3.14STLDD103 pKa = 3.35VTLTAGTGGSTDD115 pKa = 3.69FVLITSNSGEE125 pKa = 4.03EE126 pKa = 3.49TDD128 pKa = 5.63IYY130 pKa = 11.34VDD132 pKa = 3.0WDD134 pKa = 3.49YY135 pKa = 11.84DD136 pKa = 3.87NNIGNIAADD145 pKa = 3.25AWGSGTTITQDD156 pKa = 2.47SSYY159 pKa = 11.36NNTPCWEE166 pKa = 4.16LTTGDD171 pKa = 2.74GWGTVLAFMGDD182 pKa = 3.58IYY184 pKa = 11.44NVDD187 pKa = 3.9QIAEE191 pKa = 4.25FPVDD195 pKa = 3.94LTADD199 pKa = 3.72SVISFSVATTGDD211 pKa = 3.65YY212 pKa = 11.35DD213 pKa = 3.7EE214 pKa = 6.03LRR216 pKa = 11.84VKK218 pKa = 10.63VVGEE222 pKa = 4.04EE223 pKa = 4.06NEE225 pKa = 4.0KK226 pKa = 10.83EE227 pKa = 3.86IAIDD231 pKa = 3.81SFDD234 pKa = 3.62NTSTDD239 pKa = 2.9WQTVQVTTDD248 pKa = 3.16QFSEE252 pKa = 4.47VVPANVTQIAIIAFGGTAGTSKK274 pKa = 10.96VYY276 pKa = 9.4VTDD279 pKa = 3.65YY280 pKa = 9.99TISNAEE286 pKa = 3.9VVIPKK291 pKa = 8.14PQPEE295 pKa = 4.87TYY297 pKa = 9.17TLTVTVTDD305 pKa = 4.41DD306 pKa = 3.49SQVAIEE312 pKa = 4.39GATVTVNGTSKK323 pKa = 7.77TTDD326 pKa = 2.9ASGVATFDD334 pKa = 4.68LPDD337 pKa = 3.17GTYY340 pKa = 9.35TVNVSADD347 pKa = 3.29GYY349 pKa = 10.3ANGSGSVTIDD359 pKa = 3.33GADD362 pKa = 3.26KK363 pKa = 11.15SVDD366 pKa = 3.32VSLTSLEE373 pKa = 4.32VTSTLTVNVKK383 pKa = 10.21NAVSGAVIEE392 pKa = 4.69GAAVTVDD399 pKa = 3.04GTEE402 pKa = 3.64IVTDD406 pKa = 4.11ANGVAQFDD414 pKa = 4.63LVDD417 pKa = 3.72GDD419 pKa = 3.79YY420 pKa = 10.77TINVSANGYY429 pKa = 7.71NTVSQDD435 pKa = 3.24VTIAGADD442 pKa = 3.42KK443 pKa = 10.58TVDD446 pKa = 2.93ISLTTDD452 pKa = 2.96APSLFNSSFTVFNEE466 pKa = 3.9APITALQASYY476 pKa = 10.56EE477 pKa = 4.17VSTTEE482 pKa = 3.46ITEE485 pKa = 4.26GDD487 pKa = 3.55SSIKK491 pKa = 9.0VTYY494 pKa = 10.2PGDD497 pKa = 3.42NWGGIYY503 pKa = 10.29VEE505 pKa = 4.51ISTPVDD511 pKa = 3.48LSSYY515 pKa = 11.09DD516 pKa = 3.32GGNLVFDD523 pKa = 4.43IKK525 pKa = 11.17LPTTIKK531 pKa = 10.75DD532 pKa = 2.95IGVKK536 pKa = 10.56LEE538 pKa = 4.1GPKK541 pKa = 9.69GTGVQVQLANYY552 pKa = 7.46TGTDD556 pKa = 3.33AGNGWMTYY564 pKa = 8.48TIPLSDD570 pKa = 4.65LGIDD574 pKa = 3.95LTQVAVGFGLWNPTDD589 pKa = 3.81GTALDD594 pKa = 3.59AWAGGDD600 pKa = 3.63VYY602 pKa = 10.89IDD604 pKa = 3.37NVRR607 pKa = 11.84FEE609 pKa = 4.25

Molecular weight: 63.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8CWX6|B8CWX6_HALOH Cytidylate kinase OS=Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) OX=373903 GN=cmk PE=3 SV=1
MM1 pKa = 7.62AVPKK5 pKa = 10.44RR6 pKa = 11.84RR7 pKa = 11.84TSKK10 pKa = 9.52ARR12 pKa = 11.84KK13 pKa = 8.45RR14 pKa = 11.84KK15 pKa = 9.53RR16 pKa = 11.84RR17 pKa = 11.84THH19 pKa = 5.94WKK21 pKa = 9.73LKK23 pKa = 10.08SPNLVEE29 pKa = 5.51CPQCHH34 pKa = 5.44EE35 pKa = 4.93LKK37 pKa = 10.51LSHH40 pKa = 6.61RR41 pKa = 11.84VCPSCGYY48 pKa = 8.82YY49 pKa = 10.04KK50 pKa = 10.72GRR52 pKa = 11.84EE53 pKa = 4.22VVSKK57 pKa = 11.13

Molecular weight: 6.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2324

0

2324

736705

30

2277

317.0

35.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.829 ± 0.052

0.732 ± 0.017

5.512 ± 0.043

7.461 ± 0.053

4.144 ± 0.038

7.363 ± 0.046

1.576 ± 0.018

8.794 ± 0.053

7.864 ± 0.046

9.91 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.343 ± 0.021

5.209 ± 0.037

3.661 ± 0.025

2.661 ± 0.023

4.729 ± 0.035

5.399 ± 0.035

4.836 ± 0.031

7.166 ± 0.044

0.872 ± 0.022

3.94 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski