Burkholderia sp. JS23

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Chitinasiproducens

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4543 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H2PK27|A0A1H2PK27_9BURK Uncharacterized protein OS=Burkholderia sp. JS23 OX=1770053 GN=SAMN05216551_101607 PE=4 SV=1
MM1 pKa = 7.44SSSLFGIGLSGINAAQYY18 pKa = 11.6GLTTTGQNISNASTDD33 pKa = 3.03GYY35 pKa = 9.19TRR37 pKa = 11.84EE38 pKa = 4.29SVSLKK43 pKa = 10.33QSSAQYY49 pKa = 9.87TGAGYY54 pKa = 10.47LGTGVSVEE62 pKa = 4.47TVTRR66 pKa = 11.84AYY68 pKa = 10.75SQTLTTQLNNAQSTGSALDD87 pKa = 3.85AYY89 pKa = 9.21QTLSSSLDD97 pKa = 3.48SLLGDD102 pKa = 3.9PSAGIGAQMDD112 pKa = 4.01NFLTAASTVASNPSSAAARR131 pKa = 11.84EE132 pKa = 4.22SLLGQANTLASLITSTATQLQSLRR156 pKa = 11.84DD157 pKa = 3.95SVNSQLDD164 pKa = 3.74SAVSGINAAAASIASLNTQIQAASATGQTPNSLLDD199 pKa = 3.45EE200 pKa = 4.79RR201 pKa = 11.84DD202 pKa = 3.5RR203 pKa = 11.84QVAALSKK210 pKa = 9.85LVNVSVVTQSDD221 pKa = 3.47GSYY224 pKa = 10.37GVYY227 pKa = 10.14VGSGQTLVMGSRR239 pKa = 11.84SYY241 pKa = 11.77ALAAVPSTSEE251 pKa = 3.88AGEE254 pKa = 3.76QTVAYY259 pKa = 10.54VSGTGDD265 pKa = 2.9ATQYY269 pKa = 11.06LDD271 pKa = 3.73EE272 pKa = 4.64STLSGGTLGGLLSFRR287 pKa = 11.84RR288 pKa = 11.84EE289 pKa = 3.91MLDD292 pKa = 3.37PVSDD296 pKa = 3.54RR297 pKa = 11.84VGVVAAAFAGAVNGQNEE314 pKa = 4.37AGLTLGGSQGGALFTVGQPSVTAAPGNAGNAALGASIGDD353 pKa = 3.67ATALTGDD360 pKa = 4.55NYY362 pKa = 10.7TLSYY366 pKa = 10.56DD367 pKa = 3.67GKK369 pKa = 11.13SYY371 pKa = 9.41TLTNQTTGATSSPDD385 pKa = 3.29LSTGSVTVDD394 pKa = 3.01GVSYY398 pKa = 9.29TLSGTMQAGDD408 pKa = 3.85RR409 pKa = 11.84FSIAPTRR416 pKa = 11.84NAAAGFAVATSDD428 pKa = 3.18TSAIAAASPVTTAAASTNAGTGTISASADD457 pKa = 2.86ASYY460 pKa = 10.55FGSPLASAVTLSYY473 pKa = 11.09SGTDD477 pKa = 3.33GTLSGFPSAVTVSTGSSTTTYY498 pKa = 9.62PAGSAVPYY506 pKa = 9.17TAGASYY512 pKa = 11.37AFGGVTLTLAGTPKK526 pKa = 10.08TGDD529 pKa = 3.6SFTVAANTALSGDD542 pKa = 3.88GSNATAIGKK551 pKa = 9.1LADD554 pKa = 3.56AKK556 pKa = 11.06VIGGSTLSDD565 pKa = 3.29AYY567 pKa = 10.9ANLVTQVGTLAARR580 pKa = 11.84VEE582 pKa = 4.6SSNTTQTTLITSLSTQQQSVSGVNLDD608 pKa = 3.65EE609 pKa = 4.44EE610 pKa = 4.59AANLLQYY617 pKa = 9.99QQMYY621 pKa = 8.66QASSKK626 pKa = 10.25IIQTATTLFDD636 pKa = 4.42TILGLSNN643 pKa = 3.58

Molecular weight: 63.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H2PP40|A0A1H2PP40_9BURK Transcriptional regulator LysR family OS=Burkholderia sp. JS23 OX=1770053 GN=SAMN05216551_10491 PE=3 SV=1
MM1 pKa = 7.41GRR3 pKa = 11.84PGFAPAGQRR12 pKa = 11.84HH13 pKa = 4.96GRR15 pKa = 11.84NRR17 pKa = 11.84LASARR22 pKa = 11.84HH23 pKa = 5.2SRR25 pKa = 11.84RR26 pKa = 11.84QARR29 pKa = 11.84WTLSRR34 pKa = 11.84RR35 pKa = 11.84HH36 pKa = 5.73EE37 pKa = 4.29FLSEE41 pKa = 3.81HH42 pKa = 5.12NMKK45 pKa = 9.29RR46 pKa = 11.84TYY48 pKa = 10.12QPSVTRR54 pKa = 11.84RR55 pKa = 11.84KK56 pKa = 8.0RR57 pKa = 11.84THH59 pKa = 5.76GFRR62 pKa = 11.84VRR64 pKa = 11.84MKK66 pKa = 9.13TVGGRR71 pKa = 11.84KK72 pKa = 9.2VINARR77 pKa = 11.84RR78 pKa = 11.84AKK80 pKa = 9.58GRR82 pKa = 11.84KK83 pKa = 8.75RR84 pKa = 11.84LAVV87 pKa = 3.41

Molecular weight: 10.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4543

0

4543

1518797

29

9752

334.3

36.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.036 ± 0.053

0.956 ± 0.011

5.66 ± 0.035

4.843 ± 0.035

3.461 ± 0.022

8.393 ± 0.033

2.186 ± 0.015

4.258 ± 0.025

2.381 ± 0.029

10.451 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.177 ± 0.017

2.381 ± 0.022

5.308 ± 0.025

3.44 ± 0.026

7.952 ± 0.043

5.563 ± 0.033

5.315 ± 0.048

7.674 ± 0.026

1.333 ± 0.014

2.232 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski