Thermobifida halotolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Thermobifida

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4067 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A399G272|A0A399G272_9ACTN DNA helicase OS=Thermobifida halotolerans OX=483545 GN=NI17_15575 PE=3 SV=1
MM1 pKa = 7.26SKK3 pKa = 10.02VRR5 pKa = 11.84ATNRR9 pKa = 11.84RR10 pKa = 11.84SWLRR14 pKa = 11.84RR15 pKa = 11.84GFATASSLALGASMVALAAPANAAGCSVDD44 pKa = 3.52YY45 pKa = 10.63QVNSWGSGFTASVTITNLGSALSNWTLEE73 pKa = 3.46WDD75 pKa = 4.02FPGNQQVTNLWNGSYY90 pKa = 9.81SQSGQHH96 pKa = 5.44VSVDD100 pKa = 3.3NAPYY104 pKa = 9.93NGSVGEE110 pKa = 4.25GGTVNFGFNGSYY122 pKa = 10.11SGSNDD127 pKa = 2.6IPTSFSLNGVTCDD140 pKa = 3.94GSVDD144 pKa = 4.01PDD146 pKa = 4.08PPTDD150 pKa = 3.7PTDD153 pKa = 3.95PPTDD157 pKa = 3.63PTDD160 pKa = 3.95PPTDD164 pKa = 3.63PTDD167 pKa = 3.95PPTDD171 pKa = 3.63PTDD174 pKa = 3.81PPTDD178 pKa = 3.74PGEE181 pKa = 4.42RR182 pKa = 11.84VDD184 pKa = 4.09NPFVGAQGYY193 pKa = 7.83VNPVWSAKK201 pKa = 9.93AAAEE205 pKa = 4.17PGGSAVANEE214 pKa = 4.36STAVWLDD221 pKa = 3.49RR222 pKa = 11.84IGAIEE227 pKa = 4.59GNDD230 pKa = 3.48SATTGSMGLRR240 pKa = 11.84DD241 pKa = 4.31HH242 pKa = 7.21LDD244 pKa = 3.43EE245 pKa = 5.92AVAQADD251 pKa = 3.79GDD253 pKa = 4.1PLVIQVVIYY262 pKa = 9.28NLPGRR267 pKa = 11.84DD268 pKa = 3.5CAALASNGEE277 pKa = 4.27LGPEE281 pKa = 4.01EE282 pKa = 3.7IDD284 pKa = 3.36RR285 pKa = 11.84YY286 pKa = 9.4KK287 pKa = 11.04NEE289 pKa = 4.53YY290 pKa = 9.08IDD292 pKa = 5.08PIADD296 pKa = 3.08IMSDD300 pKa = 3.34YY301 pKa = 11.12AQYY304 pKa = 11.69DD305 pKa = 3.33NLRR308 pKa = 11.84IVNIIEE314 pKa = 4.22IDD316 pKa = 3.67SLPNLVTNTTDD327 pKa = 3.23NAGGTEE333 pKa = 4.44LCDD336 pKa = 3.56QMKK339 pKa = 11.14ANGNYY344 pKa = 9.64VNGVGYY350 pKa = 10.99ALATLGDD357 pKa = 3.65IPNAYY362 pKa = 10.11NYY364 pKa = 10.42VDD366 pKa = 3.89AAHH369 pKa = 6.77HH370 pKa = 6.27GWIGWDD376 pKa = 3.51TNFGPSAEE384 pKa = 4.2IFYY387 pKa = 10.41EE388 pKa = 4.09AANASGATVDD398 pKa = 4.47DD399 pKa = 3.59VHH401 pKa = 8.17GFIANTANYY410 pKa = 9.41SALEE414 pKa = 3.96EE415 pKa = 4.91PYY417 pKa = 10.82LDD419 pKa = 4.14VNGTVNGQMIRR430 pKa = 11.84QSDD433 pKa = 3.58WVDD436 pKa = 2.5WNQYY440 pKa = 8.86VDD442 pKa = 4.11EE443 pKa = 5.26LSFAQDD449 pKa = 3.18LRR451 pKa = 11.84QEE453 pKa = 4.15LVSVGFNSDD462 pKa = 2.43IGMLIDD468 pKa = 3.66TSRR471 pKa = 11.84NGWGGPEE478 pKa = 4.16RR479 pKa = 11.84PTGPSSATDD488 pKa = 3.47LNTYY492 pKa = 9.16VEE494 pKa = 4.42EE495 pKa = 4.13SRR497 pKa = 11.84IDD499 pKa = 3.37RR500 pKa = 11.84RR501 pKa = 11.84FNPGNWCNQAGAGLGEE517 pKa = 4.81RR518 pKa = 11.84PTAAPEE524 pKa = 4.12PGIDD528 pKa = 3.16AYY530 pKa = 11.33VWAKK534 pKa = 10.47PPGEE538 pKa = 3.93SDD540 pKa = 3.43GASEE544 pKa = 4.84YY545 pKa = 10.62IEE547 pKa = 4.38NDD549 pKa = 2.82EE550 pKa = 4.45GKK552 pKa = 10.7GFDD555 pKa = 4.06EE556 pKa = 4.87MCDD559 pKa = 3.29PAYY562 pKa = 10.57GGNARR567 pKa = 11.84NGNSPSGALPNAPISGHH584 pKa = 5.18WFSAQFQEE592 pKa = 4.77LLANAYY598 pKa = 8.53PPLL601 pKa = 5.25

Molecular weight: 63.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A399G4A9|A0A399G4A9_9ACTN Cytochrome P450 OS=Thermobifida halotolerans OX=483545 GN=NI17_06900 PE=3 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84FVPPSPARR10 pKa = 11.84ITPRR14 pKa = 11.84IRR16 pKa = 11.84LVTQTRR22 pKa = 11.84RR23 pKa = 11.84FVARR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 7.69TRR32 pKa = 11.84PRR34 pKa = 11.84PDD36 pKa = 3.0GGIRR40 pKa = 11.84PAAVGEE46 pKa = 3.81ARR48 pKa = 11.84YY49 pKa = 9.44RR50 pKa = 11.84CPGRR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84SGRR59 pKa = 11.84TGRR62 pKa = 11.84RR63 pKa = 11.84AAPARR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84GPLPRR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84APRR81 pKa = 11.84LRR83 pKa = 11.84PGRR86 pKa = 11.84GRR88 pKa = 11.84TAA90 pKa = 3.11

Molecular weight: 10.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4067

0

4067

1241983

34

3574

305.4

32.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.751 ± 0.056

0.754 ± 0.011

6.285 ± 0.032

6.478 ± 0.035

2.809 ± 0.021

8.931 ± 0.041

2.304 ± 0.022

3.186 ± 0.023

1.604 ± 0.021

10.381 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.767 ± 0.015

1.859 ± 0.022

5.919 ± 0.026

2.588 ± 0.021

8.763 ± 0.044

5.071 ± 0.028

5.878 ± 0.033

9.097 ± 0.042

1.493 ± 0.018

2.083 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski