Flavobacterium tangerina
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3128 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3P3WA51|A0A3P3WA51_9FLAO 30S ribosomal protein S17 OS=Flavobacterium tangerina OX=2488728 GN=rpsQ PE=3 SV=1
MM1 pKa = 7.58 KK2 pKa = 10.39 KK3 pKa = 8.68 NTILLVIAFFGSITINAQTIASEE26 pKa = 4.17 NFEE29 pKa = 4.43 TTGLYY34 pKa = 10.16 DD35 pKa = 5.18 LPVGWFANGDD45 pKa = 3.54 FFYY48 pKa = 11.13 DD49 pKa = 3.77 SFVDD53 pKa = 4.89 DD54 pKa = 4.52 YY55 pKa = 12.01 YY56 pKa = 11.61 GGCEE60 pKa = 4.05 DD61 pKa = 3.73 EE62 pKa = 4.88 QYY64 pKa = 10.92 IYY66 pKa = 11.26 TNLFEE71 pKa = 5.9 SDD73 pKa = 3.32 SQLFLTSQNYY83 pKa = 10.35 LNLNAGPKK91 pKa = 8.64 QVSYY95 pKa = 9.05 QLRR98 pKa = 11.84 VIDD101 pKa = 4.57 YY102 pKa = 10.59 DD103 pKa = 4.16 DD104 pKa = 4.38 EE105 pKa = 5.21 VPVNYY110 pKa = 10.21 DD111 pKa = 3.18 FGSITFSYY119 pKa = 10.54 SLNDD123 pKa = 3.39 GVTWIVLGSVSEE135 pKa = 4.97 DD136 pKa = 2.99 NFTPSLACQEE146 pKa = 3.52 ISYY149 pKa = 9.23 TIEE152 pKa = 4.14 GSLIAAQSNLKK163 pKa = 10.06 FKK165 pKa = 10.44 WEE167 pKa = 3.93 SAYY170 pKa = 11.02 SGEE173 pKa = 4.0 GDD175 pKa = 3.99 YY176 pKa = 11.21 EE177 pKa = 3.95 ILIDD181 pKa = 4.49 NFMLSEE187 pKa = 4.17 SQTASTNEE195 pKa = 3.71 LDD197 pKa = 3.69 KK198 pKa = 11.33 SQLKK202 pKa = 10.08 IYY204 pKa = 7.98 PNPVSDD210 pKa = 4.58 VLNIDD215 pKa = 3.57 YY216 pKa = 10.87 SSTISKK222 pKa = 9.66 FIIFDD227 pKa = 3.6 LVGRR231 pKa = 11.84 KK232 pKa = 8.97 IGEE235 pKa = 4.02 FSNSGNLNSIDD246 pKa = 3.77 VSNLSTGTYY255 pKa = 9.5 LLKK258 pKa = 10.43 IQTEE262 pKa = 4.5 DD263 pKa = 3.3 NSQSTIKK270 pKa = 10.42 FIKK273 pKa = 9.94 KK274 pKa = 9.77
Molecular weight: 30.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.784
IPC2_protein 3.935
IPC_protein 3.923
Toseland 3.719
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.821
Rodwell 3.745
Grimsley 3.617
Solomon 3.897
Lehninger 3.859
Nozaki 4.012
DTASelect 4.24
Thurlkill 3.757
EMBOSS 3.834
Sillero 4.037
Patrickios 0.731
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.926
Protein with the highest isoelectric point:
>tr|A0A3P3W2K8|A0A3P3W2K8_9FLAO Pseudouridylate synthase OS=Flavobacterium tangerina OX=2488728 GN=EG240_13850 PE=4 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.33 KK3 pKa = 10.41 SILIKK8 pKa = 10.35 IFGLVFIFTLCLQSCGSFNNNPQRR32 pKa = 11.84 EE33 pKa = 4.02 VDD35 pKa = 3.38 RR36 pKa = 11.84 QQNQNSTIRR45 pKa = 11.84 NNTSRR50 pKa = 11.84 NDD52 pKa = 3.42 SNRR55 pKa = 11.84 GSNSNNSRR63 pKa = 11.84 EE64 pKa = 4.26 NNTSNRR70 pKa = 11.84 NNSNRR75 pKa = 11.84 NTEE78 pKa = 4.22 QKK80 pKa = 10.64 SEE82 pKa = 3.87 TRR84 pKa = 11.84 TSEE87 pKa = 3.58 EE88 pKa = 3.91 RR89 pKa = 11.84 KK90 pKa = 9.94 IKK92 pKa = 10.62
Molecular weight: 10.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.299
IPC2_protein 9.443
IPC_protein 9.794
Toseland 10.862
ProMoST 10.555
Dawson 10.891
Bjellqvist 10.555
Wikipedia 11.067
Rodwell 11.155
Grimsley 10.906
Solomon 11.052
Lehninger 11.038
Nozaki 10.847
DTASelect 10.555
Thurlkill 10.833
EMBOSS 11.257
Sillero 10.833
Patrickios 10.935
IPC_peptide 11.067
IPC2_peptide 9.575
IPC2.peptide.svr19 8.73
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3128
0
3128
991835
34
2656
317.1
36.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.803 ± 0.042
0.773 ± 0.013
5.384 ± 0.031
6.843 ± 0.048
5.698 ± 0.04
5.774 ± 0.049
1.604 ± 0.022
8.578 ± 0.051
8.169 ± 0.052
9.18 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.158 ± 0.021
6.985 ± 0.049
3.151 ± 0.026
3.564 ± 0.028
3.062 ± 0.03
6.433 ± 0.037
5.626 ± 0.046
6.038 ± 0.04
0.935 ± 0.015
4.242 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here