Apple luteovirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Luteovirus

Average proteome isoelectric point is 7.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8N4X5|A0A2U8N4X5_9LUTE RNA-directed RNA polymerase OS=Apple luteovirus 1 OX=2170544 GN=ORF1-ORF2 PE=4 SV=1
MM1 pKa = 7.5LFDD4 pKa = 5.91DD5 pKa = 6.15LICASFKK12 pKa = 10.26VVKK15 pKa = 10.57DD16 pKa = 5.11FISHH20 pKa = 7.01IYY22 pKa = 9.59NNLRR26 pKa = 11.84TVYY29 pKa = 10.48KK30 pKa = 10.4KK31 pKa = 10.57FKK33 pKa = 8.49VWLWEE38 pKa = 3.97LQGKK42 pKa = 9.06FSQHH46 pKa = 6.26DD47 pKa = 3.83AFVDD51 pKa = 3.57ACYY54 pKa = 10.56GYY56 pKa = 9.9MDD58 pKa = 5.35DD59 pKa = 5.06VEE61 pKa = 4.32QFEE64 pKa = 4.58WDD66 pKa = 4.03CYY68 pKa = 10.29SAYY71 pKa = 11.01NDD73 pKa = 4.09ADD75 pKa = 3.71VEE77 pKa = 4.16LALARR82 pKa = 11.84LHH84 pKa = 7.11LDD86 pKa = 3.64TVLKK90 pKa = 10.38APKK93 pKa = 8.19VTGWPVPTRR102 pKa = 11.84PDD104 pKa = 3.43GAPTTTEE111 pKa = 3.91VPKK114 pKa = 11.07YY115 pKa = 7.4PTLHH119 pKa = 6.33EE120 pKa = 4.28LAEE123 pKa = 4.61KK124 pKa = 10.38IRR126 pKa = 11.84TSVRR130 pKa = 11.84RR131 pKa = 11.84EE132 pKa = 3.6RR133 pKa = 11.84VFQAAGEE140 pKa = 4.01ASGDD144 pKa = 3.32KK145 pKa = 10.61DD146 pKa = 3.66AEE148 pKa = 4.42PEE150 pKa = 4.26SEE152 pKa = 4.53VPEE155 pKa = 4.63GWHH158 pKa = 5.06VDD160 pKa = 3.19VWNKK164 pKa = 9.68FQDD167 pKa = 3.51EE168 pKa = 4.24EE169 pKa = 3.96RR170 pKa = 11.84TYY172 pKa = 10.5WEE174 pKa = 4.19NYY176 pKa = 6.99YY177 pKa = 11.23ANPIEE182 pKa = 5.11LAPQVIPVNPGVAEE196 pKa = 4.03PPMPKK201 pKa = 9.55PIYY204 pKa = 7.5TEE206 pKa = 4.12RR207 pKa = 11.84VAFTEE212 pKa = 4.33DD213 pKa = 3.19EE214 pKa = 4.3LFKK217 pKa = 11.42AEE219 pKa = 4.71ARR221 pKa = 11.84LTRR224 pKa = 11.84AKK226 pKa = 10.1RR227 pKa = 11.84SYY229 pKa = 10.98SSTIEE234 pKa = 4.53DD235 pKa = 3.32IKK237 pKa = 11.21DD238 pKa = 3.17QYY240 pKa = 11.33EE241 pKa = 4.06EE242 pKa = 4.38EE243 pKa = 4.19KK244 pKa = 11.24GEE246 pKa = 4.46GYY248 pKa = 10.59FGRR251 pKa = 11.84FFNTFEE257 pKa = 4.01QRR259 pKa = 11.84MHH261 pKa = 5.13YY262 pKa = 9.81VKK264 pKa = 10.57RR265 pKa = 11.84ARR267 pKa = 11.84SRR269 pKa = 11.84RR270 pKa = 11.84AKK272 pKa = 9.74TDD274 pKa = 3.39QLCHH278 pKa = 6.3KK279 pKa = 9.62VQGKK283 pKa = 9.51LSQVAEE289 pKa = 4.16LPDD292 pKa = 4.75FYY294 pKa = 11.11EE295 pKa = 4.27LCTVRR300 pKa = 11.84EE301 pKa = 4.16VEE303 pKa = 3.93TGEE306 pKa = 3.91FHH308 pKa = 6.69TVMDD312 pKa = 4.2EE313 pKa = 4.03GEE315 pKa = 4.28EE316 pKa = 3.87IKK318 pKa = 10.84RR319 pKa = 11.84PIVKK323 pKa = 9.84VSRR326 pKa = 11.84SIKK329 pKa = 9.28PEE331 pKa = 3.73CRR333 pKa = 11.84RR334 pKa = 11.84DD335 pKa = 3.35AQSYY339 pKa = 7.03IRR341 pKa = 11.84KK342 pKa = 9.68YY343 pKa = 10.28IRR345 pKa = 11.84SKK347 pKa = 10.54NNRR350 pKa = 11.84VGADD354 pKa = 3.63EE355 pKa = 5.01IGVATINRR363 pKa = 11.84YY364 pKa = 8.06VAQFADD370 pKa = 4.54DD371 pKa = 4.23MKK373 pKa = 11.55LDD375 pKa = 3.73MASSEE380 pKa = 4.15FLARR384 pKa = 11.84TALTIVPVVTKK395 pKa = 10.69QEE397 pKa = 4.0MVQAMVIHH405 pKa = 6.42SPAARR410 pKa = 11.84KK411 pKa = 9.31ARR413 pKa = 11.84ADD415 pKa = 3.54LAALEE420 pKa = 4.61GQDD423 pKa = 3.58FF424 pKa = 3.83

Molecular weight: 48.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8N4S3|A0A2U8N4S3_9LUTE Readthrough protein OS=Apple luteovirus 1 OX=2170544 GN=ORF3-ORF5 PE=3 SV=1
MM1 pKa = 7.36TPSSMPVTVTWTTSSSSSGTATQHH25 pKa = 5.61TMMLMLNLPSPDD37 pKa = 3.51STSTPSLRR45 pKa = 11.84LLKK48 pKa = 10.72LPAGPSQQDD57 pKa = 3.14LMVRR61 pKa = 11.84QPRR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84SPNTRR71 pKa = 11.84LCTNSQKK78 pKa = 10.59RR79 pKa = 11.84SAQACDD85 pKa = 2.83EE86 pKa = 4.18SGYY89 pKa = 11.07FKK91 pKa = 11.05LQVKK95 pKa = 10.12RR96 pKa = 11.84LVIKK100 pKa = 8.36MQNLSLKK107 pKa = 8.14CQRR110 pKa = 11.84GGTLTSGTSSKK121 pKa = 9.36TRR123 pKa = 11.84SEE125 pKa = 4.14PTGKK129 pKa = 7.33TTTPTPSSWRR139 pKa = 11.84RR140 pKa = 11.84RR141 pKa = 11.84LYY143 pKa = 11.05LSTQGSLNLPCPSRR157 pKa = 11.84YY158 pKa = 7.92TPRR161 pKa = 11.84EE162 pKa = 3.67SPLRR166 pKa = 11.84RR167 pKa = 11.84TSSSKK172 pKa = 10.61LRR174 pKa = 11.84LGSPGQNVHH183 pKa = 5.65TRR185 pKa = 11.84PPLKK189 pKa = 9.94TSRR192 pKa = 11.84TSMKK196 pKa = 9.92KK197 pKa = 8.82RR198 pKa = 11.84RR199 pKa = 11.84EE200 pKa = 4.12RR201 pKa = 11.84ATSAAFLTRR210 pKa = 11.84LSSEE214 pKa = 4.18CIMLRR219 pKa = 11.84EE220 pKa = 4.08RR221 pKa = 11.84GAVEE225 pKa = 3.82PRR227 pKa = 11.84RR228 pKa = 11.84TSCVTRR234 pKa = 11.84FKK236 pKa = 11.11VNSVRR241 pKa = 11.84LLNYY245 pKa = 9.33LISMSCVPSEE255 pKa = 3.44KK256 pKa = 9.56WKK258 pKa = 10.11PVSSTLL264 pKa = 3.99

Molecular weight: 29.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

3024

38

953

302.4

34.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.713 ± 0.694

1.72 ± 0.221

5.126 ± 0.866

6.118 ± 1.057

3.935 ± 0.562

5.754 ± 0.523

1.62 ± 0.247

4.497 ± 0.671

6.052 ± 0.707

7.573 ± 1.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.513 ± 0.484

4.134 ± 0.532

6.581 ± 0.808

3.472 ± 0.231

8.366 ± 0.894

8.267 ± 1.735

5.721 ± 0.893

7.044 ± 0.732

1.389 ± 0.144

3.373 ± 0.455
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski