Tetrasphaera jenkinsii Ben 74
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3715 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A077ME16|A0A077ME16_9MICO Uncharacterized protein OS=Tetrasphaera jenkinsii Ben 74 OX=1193518 GN=BN13_30045 PE=4 SV=1
MM1 pKa = 7.83 SDD3 pKa = 3.68 FDD5 pKa = 4.23 YY6 pKa = 11.23 PDD8 pKa = 3.9 DD9 pKa = 3.81 LRR11 pKa = 11.84 YY12 pKa = 7.76 TAEE15 pKa = 4.29 HH16 pKa = 5.61 EE17 pKa = 4.48 WVRR20 pKa = 11.84 SVGDD24 pKa = 3.15 GRR26 pKa = 11.84 VRR28 pKa = 11.84 VGITAYY34 pKa = 10.48 AQDD37 pKa = 3.65 ALGDD41 pKa = 4.07 VVFVTAPAVGDD52 pKa = 4.15 SISAGDD58 pKa = 3.44 ACGEE62 pKa = 4.05 IEE64 pKa = 4.32 STKK67 pKa = 10.64 SVSDD71 pKa = 4.2 LYY73 pKa = 11.6 SPLTGEE79 pKa = 3.83 ITAVNAALDD88 pKa = 3.87 ATPEE92 pKa = 4.85 LINSDD97 pKa = 3.79 PYY99 pKa = 11.13 GEE101 pKa = 4.48 GWCFEE106 pKa = 4.11 MSLADD111 pKa = 4.85 AADD114 pKa = 4.44 LDD116 pKa = 4.3 SLMDD120 pKa = 4.04 AAAYY124 pKa = 9.5 RR125 pKa = 11.84 DD126 pKa = 4.01 SVGG129 pKa = 3.2
Molecular weight: 13.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.617
IPC_protein 3.617
Toseland 3.389
ProMoST 3.808
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.592
Rodwell 3.439
Grimsley 3.3
Solomon 3.605
Lehninger 3.567
Nozaki 3.745
DTASelect 3.999
Thurlkill 3.465
EMBOSS 3.592
Sillero 3.745
Patrickios 0.896
IPC_peptide 3.592
IPC2_peptide 3.706
IPC2.peptide.svr19 3.689
Protein with the highest isoelectric point:
>tr|A0A077M9L8|A0A077M9L8_9MICO Ribonuclease VapC OS=Tetrasphaera jenkinsii Ben 74 OX=1193518 GN=vapC PE=3 SV=1
MM1 pKa = 7.84 PPTARR6 pKa = 11.84 RR7 pKa = 11.84 SLRR10 pKa = 11.84 SQTLMFRR17 pKa = 11.84 MIRR20 pKa = 11.84 LAPLAIVRR28 pKa = 11.84 GFAPRR33 pKa = 11.84 MPPKK37 pKa = 10.15 ARR39 pKa = 11.84 RR40 pKa = 11.84 SLRR43 pKa = 11.84 SQTLMFRR50 pKa = 11.84 TIRR53 pKa = 11.84 LAPLAIVRR61 pKa = 11.84 RR62 pKa = 11.84 FAIVPGTPQPSSRR75 pKa = 11.84 RR76 pKa = 11.84 WTTSSS81 pKa = 2.95
Molecular weight: 9.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.403
IPC_protein 13.013
Toseland 13.173
ProMoST 13.671
Dawson 13.173
Bjellqvist 13.173
Wikipedia 13.656
Rodwell 12.705
Grimsley 13.217
Solomon 13.671
Lehninger 13.583
Nozaki 13.173
DTASelect 13.173
Thurlkill 13.173
EMBOSS 13.671
Sillero 13.173
Patrickios 12.427
IPC_peptide 13.685
IPC2_peptide 12.676
IPC2.peptide.svr19 9.316
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3715
0
3715
1123227
11
2043
302.3
32.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.612 ± 0.063
0.76 ± 0.01
6.202 ± 0.036
5.162 ± 0.037
2.818 ± 0.025
9.158 ± 0.04
2.156 ± 0.023
4.114 ± 0.03
2.145 ± 0.033
9.987 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.95 ± 0.018
1.942 ± 0.026
5.78 ± 0.033
2.73 ± 0.021
7.687 ± 0.052
5.524 ± 0.035
6.157 ± 0.037
8.586 ± 0.043
1.57 ± 0.019
1.959 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here