Aruac virus
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R1D3|A0A0D3R1D3_9RHAB Nucleocapsid protein OS=Aruac virus OX=1272961 PE=4 SV=1
MM1 pKa = 7.83 PNYY4 pKa = 9.84 FSPPVQQPHH13 pKa = 6.94 TINLPFSLEE22 pKa = 3.85 VHH24 pKa = 7.06 IIACGDD30 pKa = 3.2 NMMTLSLEE38 pKa = 3.91 PHH40 pKa = 5.51 QNEE43 pKa = 4.52 INLSSYY49 pKa = 10.27 EE50 pKa = 4.1 KK51 pKa = 10.94 NN52 pKa = 3.32
Molecular weight: 5.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.905
IPC2_protein 5.016
IPC_protein 4.762
Toseland 4.762
ProMoST 4.914
Dawson 4.762
Bjellqvist 4.889
Wikipedia 4.635
Rodwell 4.711
Grimsley 4.711
Solomon 4.762
Lehninger 4.711
Nozaki 4.914
DTASelect 4.965
Thurlkill 4.787
EMBOSS 4.685
Sillero 4.978
Patrickios 0.693
IPC_peptide 4.774
IPC2_peptide 4.978
IPC2.peptide.svr19 4.909
Protein with the highest isoelectric point:
>tr|A0A0D3R1I2|A0A0D3R1I2_9RHAB Uncharacterized protein OS=Aruac virus OX=1272961 PE=4 SV=1
MM1 pKa = 7.51 HH2 pKa = 6.83 NQTRR6 pKa = 11.84 AVVSFPNLPNVGAFADD22 pKa = 4.27 SIKK25 pKa = 10.04 NTLEE29 pKa = 3.88 EE30 pKa = 3.63 TWIRR34 pKa = 11.84 FVQKK38 pKa = 10.21 IEE40 pKa = 4.05 SWSDD44 pKa = 3.24 TVKK47 pKa = 9.29 QTLIVVFIIICIFIAIKK64 pKa = 10.32 ILIVCGQLCTTICQGTTHH82 pKa = 7.31 GLNCIRR88 pKa = 11.84 SCSRR92 pKa = 11.84 RR93 pKa = 11.84 RR94 pKa = 11.84 ILRR97 pKa = 11.84 PSPPPKK103 pKa = 9.51 PKK105 pKa = 9.83 RR106 pKa = 11.84 RR107 pKa = 11.84 KK108 pKa = 7.71 QTSAPDD114 pKa = 3.17 RR115 pKa = 11.84 YY116 pKa = 10.01 YY117 pKa = 11.05 GYY119 pKa = 11.28 AVTQVV124 pKa = 2.83
Molecular weight: 14.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.232
IPC2_protein 9.209
IPC_protein 9.282
Toseland 9.999
ProMoST 9.677
Dawson 10.189
Bjellqvist 9.926
Wikipedia 10.335
Rodwell 10.57
Grimsley 10.233
Solomon 10.233
Lehninger 10.218
Nozaki 10.16
DTASelect 9.853
Thurlkill 10.058
EMBOSS 10.409
Sillero 10.145
Patrickios 10.292
IPC_peptide 10.233
IPC2_peptide 9.18
IPC2.peptide.svr19 7.978
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
3854
52
2100
550.6
62.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.359 ± 0.922
1.687 ± 0.292
5.397 ± 0.421
6.098 ± 0.5
3.866 ± 0.204
5.942 ± 0.452
2.128 ± 0.411
8.199 ± 0.868
6.565 ± 0.178
9.549 ± 1.272
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.673 ± 0.432
4.774 ± 0.459
5.138 ± 0.859
3.477 ± 0.217
4.956 ± 0.38
8.459 ± 0.706
5.942 ± 0.402
5.189 ± 0.811
1.92 ± 0.238
3.684 ± 0.372
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here