Mucilaginibacter polytrichastri

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Mucilaginibacter

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5195 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q6A4D0|A0A1Q6A4D0_9SPHI Uncharacterized protein OS=Mucilaginibacter polytrichastri OX=1302689 GN=RG47T_4331 PE=4 SV=1
MM1 pKa = 8.19SMVPFFLEE9 pKa = 4.62LEE11 pKa = 4.44LDD13 pKa = 3.78NKK15 pKa = 10.18PLIIAVEE22 pKa = 3.94QLQNFADD29 pKa = 3.41VDD31 pKa = 4.55GYY33 pKa = 11.23CRR35 pKa = 11.84YY36 pKa = 10.57DD37 pKa = 2.86ITAAEE42 pKa = 4.18RR43 pKa = 11.84RR44 pKa = 11.84AVVYY48 pKa = 10.87VNLEE52 pKa = 4.09YY53 pKa = 10.75EE54 pKa = 4.36DD55 pKa = 4.19LQLPVSPQDD64 pKa = 3.24LEE66 pKa = 4.36TYY68 pKa = 10.29YY69 pKa = 10.81EE70 pKa = 4.62AIHH73 pKa = 6.15YY74 pKa = 8.13PEE76 pKa = 4.02QAQAFITDD84 pKa = 3.75DD85 pKa = 4.49DD86 pKa = 4.41EE87 pKa = 5.54LFSTKK92 pKa = 9.42EE93 pKa = 3.82FNVIAAAIRR102 pKa = 11.84QYY104 pKa = 11.14NRR106 pKa = 11.84EE107 pKa = 4.01AGMSFPEE114 pKa = 4.57FNFDD118 pKa = 3.86LL119 pKa = 4.82

Molecular weight: 13.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q6A457|A0A1Q6A457_9SPHI Uncharacterized protein OS=Mucilaginibacter polytrichastri OX=1302689 GN=RG47T_4277 PE=4 SV=1
MM1 pKa = 7.52NFNKK5 pKa = 10.18IIIAAALTVSSIGLVEE21 pKa = 4.32AAPLKK26 pKa = 10.25AAPAVAAATLQLPPPPPRR44 pKa = 11.84PPLPRR49 pKa = 11.84FRR51 pKa = 11.84RR52 pKa = 11.84HH53 pKa = 4.84NTRR56 pKa = 11.84HH57 pKa = 6.17HH58 pKa = 5.55YY59 pKa = 7.45RR60 pKa = 11.84TRR62 pKa = 11.84RR63 pKa = 11.84XXXXXXTLAATAASPAASKK82 pKa = 9.76VVKK85 pKa = 9.98YY86 pKa = 11.0NCDD89 pKa = 3.22LLFFIRR95 pKa = 11.84STGLFGAAFFLSQCANLLYY114 pKa = 10.86LNSPNLCPKK123 pKa = 8.36MTFCFSTDD131 pKa = 2.99TRR133 pKa = 11.84NLATHH138 pKa = 6.51VNTT141 pKa = 5.06

Molecular weight: 14.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5195

0

5195

1703259

37

4093

327.9

36.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.82 ± 0.039

0.758 ± 0.01

5.41 ± 0.025

5.218 ± 0.041

4.783 ± 0.025

6.808 ± 0.037

1.97 ± 0.015

7.372 ± 0.029

6.897 ± 0.034

9.473 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.281 ± 0.017

5.751 ± 0.032

3.867 ± 0.019

3.929 ± 0.02

3.71 ± 0.019

6.163 ± 0.027

6.041 ± 0.041

6.319 ± 0.025

1.131 ± 0.012

4.267 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski