Cercospora zeina
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10192 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I0S9N7|A0A2I0S9N7_9PEZI Putative oxidoreductase YkvO OS=Cercospora zeina OX=348901 GN=BST61_czeina2g001900 PE=4 SV=1
MM1 pKa = 7.28 FLSTAATAIALLASTVAAIPASLTSMAEE29 pKa = 4.03 NPNLEE34 pKa = 3.89 ITEE37 pKa = 4.13 LVINSTANGNTMLQFNVQNPEE58 pKa = 3.66 PLAANGIAEE67 pKa = 5.14 CRR69 pKa = 11.84 GEE71 pKa = 4.04 WDD73 pKa = 3.34 TTKK76 pKa = 10.61 QDD78 pKa = 3.14 WPISGEE84 pKa = 4.58 ALTCDD89 pKa = 3.97 NSTSMTWYY97 pKa = 9.2 IDD99 pKa = 3.0 SWTSAMNFVINIQDD113 pKa = 3.57 RR114 pKa = 11.84 FDD116 pKa = 4.33 DD117 pKa = 4.39 PSVGKK122 pKa = 9.38 PPYY125 pKa = 10.9 DD126 pKa = 3.89 RR127 pKa = 11.84 MTTFSKK133 pKa = 10.95 AIINEE138 pKa = 4.37 TVVSYY143 pKa = 11.35 ASDD146 pKa = 3.58 TPAGNTNAGTDD157 pKa = 3.94 APPEE161 pKa = 4.63 SISGSQLPDD170 pKa = 2.83 ITIYY174 pKa = 10.9 AVVYY178 pKa = 10.24 AVSAA182 pKa = 3.86
Molecular weight: 19.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.77
IPC_protein 3.732
Toseland 3.516
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.439
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.776
Protein with the highest isoelectric point:
>tr|A0A2I0RS63|A0A2I0RS63_9PEZI DNA replication checkpoint protein tel2 OS=Cercospora zeina OX=348901 GN=BST61_czeina50g000040 PE=3 SV=1
MM1 pKa = 6.82 VTGQRR6 pKa = 11.84 ACVRR10 pKa = 11.84 RR11 pKa = 11.84 VRR13 pKa = 11.84 RR14 pKa = 11.84 MWCRR18 pKa = 11.84 RR19 pKa = 11.84 KK20 pKa = 9.77 AVGWTKK26 pKa = 9.36 HH27 pKa = 5.17 TICRR31 pKa = 11.84 HH32 pKa = 6.03 DD33 pKa = 3.85 AFQYY37 pKa = 7.49 ATPDD41 pKa = 3.45 MQRR44 pKa = 11.84 VKK46 pKa = 10.76 YY47 pKa = 10.0 HH48 pKa = 5.95 VEE50 pKa = 3.42 ASLRR54 pKa = 11.84 SINVEE59 pKa = 4.15 VQQHH63 pKa = 4.21 VAKK66 pKa = 10.7 SRR68 pKa = 11.84 IAGAGASNVSFAGGSPTCCCWISDD92 pKa = 3.83 AVGLKK97 pKa = 10.33 NIWPWQSSRR106 pKa = 11.84 GQWRR110 pKa = 11.84 TLVIMRR116 pKa = 11.84 TGGSNRR122 pKa = 11.84 SPEE125 pKa = 4.15 AASHH129 pKa = 6.73 CDD131 pKa = 3.29 ATFHH135 pKa = 6.15 GQQ137 pKa = 2.94
Molecular weight: 15.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.443
IPC_protein 10.072
Toseland 10.526
ProMoST 10.204
Dawson 10.628
Bjellqvist 10.379
Wikipedia 10.833
Rodwell 10.804
Grimsley 10.672
Solomon 10.745
Lehninger 10.716
Nozaki 10.613
DTASelect 10.335
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.584
Patrickios 10.57
IPC_peptide 10.76
IPC2_peptide 9.882
IPC2.peptide.svr19 8.479
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10192
0
10192
5190240
58
8800
509.2
56.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.358 ± 0.02
1.221 ± 0.009
5.744 ± 0.018
6.192 ± 0.024
3.507 ± 0.014
6.912 ± 0.022
2.544 ± 0.011
4.545 ± 0.015
4.812 ± 0.023
8.547 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.184 ± 0.009
3.6 ± 0.012
6.03 ± 0.025
4.306 ± 0.018
6.33 ± 0.021
8.007 ± 0.026
6.053 ± 0.02
6.001 ± 0.015
1.428 ± 0.009
2.675 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here